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Potential basis of glyphosate resistance in California rigid ryegrass (Lolium rigidum)

  • Marulak Simarmata (a1), John E. Kaufmann (a1) and Donald Penner

Glyphosate-resistant rigid ryegrass has been identified in California, but research has yet to elucidate the resistance mechanism. The objectives of this study were to examine the differences between sensitive and resistant rigid ryegrass in absorption and distribution of glyphosate, in vivo and in vitro absorption by chloroplasts, and shikimic acid accumulation after glyphosate treatment. Foliar absorption and distribution of 14C-glyphosate did not differ 1 to 3 d after treatment (DAT) between the susceptible (S) and resistant (R) biotypes. Absorption of 14C-glyphosate by isolated chloroplasts also did not differ between the S and R biotypes. After foliar application of 14C-glyphosate, chloroplasts were isolated from treated leaves from both biotypes. Accumulation of 14C-glyphosate in the chloroplasts did not differ between the two biotypes. Shikimic acid level increased significantly in the S biotype after treatment with glyphosate at 2.24 kg ai ha−1 to levels 10-fold greater than in the R biotype 11 DAT. Shikimic acid in the germination media at 2 to 5 mM did not affect seed germination of S and R biotypes but drastically decreased the length of coleoptiles of both at 5 DAT. Thus, biotype differences in sensitivity or metabolism of shikimic acid do not explain differences in sensitivity to glyphosate.

Corresponding author
Corresponding author. Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824;
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Ahrens W. H. ed. 1994. Herbicide Handbook. 7th ed. Champaign, IL: Weed Science Society of America. Pp. 149152.
Baird D. D., Upchurch R. P., Homesly W. B., and Franz J. E. 1971. Introduction of a new broadspectrum postemergence herbicide class with utility for herbaceous perennial weed control. Proc. N. Cent. Weed Conf 26:6468.
Bradshaw L. D., Padgette S. R., Kimball S. L., and Wells B. H. 1997. Perspective on glyphosate resistance. Weed Technol 11:189198.
Coupland D. 1985. The effect of temperature on the activity and metabolism of glyphosate applied to rhizome fragments of Elymus repens (=Agropyron repens). Pestic. Sci 15:226234.
Dyer W. E. 1994. Resistance to glyphosate. Pages 229241 in Powles S. B. and Holtum J.A.M. eds. Herbicide Resistance in Plants, Biology and Biochemistry. Boca Raton, FL: CRC Press.
Feng P. C. C., Pratley J. E., and Bohn J. A. 1999. Resistance to glyphosate in Lolium rigidum II. Uptake, translocation and metabolism. Weed Sci 47:412415.
Gaitonde M. K. and Gordon M. W. 1957. A microchemical method for the detection and determination of shikimic acid. J. Biol. Chem 230:10431050.
Harring T., Streibig J. C., and Husted S. 1998. Accumulation of shikimic acid: a technique for screening glyphosate efficacy. J. Agric. Food Chem 46:44064412.
Kishore G. M., Padgette S. R., and Fraley R. T. 1992. History of herbicide tolerant crops, methods of development and current state of the art—emphasis on glyphosate tolerance. Weed Technol 6:626634.
Lee L. J. and Ngim J. 2000. A first report of glyphosate-resistant goosegrass (Eleusine indica (L.) Gaertn) in Malaysia. Pestic. Manag. Sci 56:336339.
Lorraine-Colwill D. F., Hawkes T. R., Williams P. H., Warner S. A. J., Sutton P. B., Powles S. B., and Preston C. 1999. Resistance to glyphosate in Lolium rigidum . Pestic. Sci 55:486503.
Moreland D. E. 1986. Measurements of reactions mediated by isolated chloroplast. Pages 309324 in Camper N. D., ed. Research Methods in Weed Sciences. 3rd ed. Champaign, IL: Southern Weed Science Society.
Padgette S. R., Re D. B., Baray G. F., Eicholtz D. E., Delanay X., Fuchs R. L., Kishore G. M., and Fraley R. T. 1996. New weed control opportunities development of soybeans with a Roundup Ready gene. Pages 5389 in Duke S. O. ed. Herbicide Resistant Crops. Boca Raton, FL: CRC Press.
Pline W. A., Wilcut J. W., Duke S. O., Edmisten K. L., and Wells R. 2002. Tolerance and accumulation of shikimic acid in response to glyphosate applications in glyphosate resistant and non-glyphosate resistant cotton (Gossypium hirsutum L). J. Agric. Food Chem 50:506512.
Powles S. B., Lorraine-Colwill D. F., Dellow J. J., and Preston C. 1998. Evolved resistance to glyphosate in rigid ryegrass (Lolium rigidum) in Australia. Weed Sci 46:604607.
Powles S. B., Preston C., Bryan I. B., and Justum A. R. 1997. Herbicide resistance: impact and management. Adv. Agron 58:5793.
Pratley J., Urwin N., Stanton R., Baines P., Broster J., Cullis K., Schafer D., Bohn J., and Krueger J. 1999. Resistance to glyphosate in Lolium rigidum. I. Bioevaluation. Weed Sci 47:405411.
Sandberg C. L., Meggitt W. F., and Penner D. 1980. Absorption, translocation and metabolism of 14C-glyphosate in several weed species. Weed Res 20:195200.
Siehl D. L. 1997. Inhibitor of EPSP synthase, glutamine synthetase and histidine synthesis. Pages 3767 in Roe R. M. ed. Herbicide Activity: Toxicology, Biochemistry and Molecular Biology. Amsterdam: IOS Press.
Simarmata M., Kaufmann J. E., and Penner D. 2001. Progress in determining the origin of the glyphosate-resistant ryegrass in California. 2001 WSSA Abstr 41:9596.
Singh B. K. and Shaner D. L. 1998. Rapid determination of glyphosate injury to plants and identification of glyphosate resistant plants. Weed Technol 12:527530.
Steinrucken H. C. and Amrhein N. 1980. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimate acid-3-phosphate synthase. Biochem. Biophys. Res. Commun 94:12071212.
Tran M., Baerson S., and Brinker R. et al. 1999. Characterization of glyphosate resistant Eleusine indica biotypes from Malaysia. Pages 527536 in Proceedings of the 17th Asian-Pacific Weed Science Society Conference; Bangkok, Thailand. Bangkok, Thailand. Asian-Pacific Weed Science Society.
VanGessel M. S. 2002. Glyphosate-resistant horseweed in the Delmarva peninsula: status and management. Proc. N. Cent. Weed Sci. Soc. 57. [CD-ROM].
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Weed Science
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