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A Rare Ile-2041-Thr Mutation in the ACCase Gene Confers Resistance to ACCase-inhibiting Herbicides in Shortawn Foxtail (Alopecurus aequalis)

Published online by Cambridge University Press:  08 February 2017

Wenlei Guo ,
Lele Zhang ,
Hengzhi Wang ,
Qi Li ,
Weitang Liu  and
Jinxin Wang
Wenlei Guo
Affiliation:
Graduate Student, Graduate Student, Graduate Student, Graduate Student, Lecturer and Professor, Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai’an 271018, People’s Republic of China
Lele Zhang
Affiliation:
Graduate Student, Graduate Student, Graduate Student, Graduate Student, Lecturer and Professor, Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai’an 271018, People’s Republic of China
Hengzhi Wang
Affiliation:
Graduate Student, Graduate Student, Graduate Student, Graduate Student, Lecturer and Professor, Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai’an 271018, People’s Republic of China
Qi Li
Affiliation:
Graduate Student, Graduate Student, Graduate Student, Graduate Student, Lecturer and Professor, Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai’an 271018, People’s Republic of China
Weitang Liu
Affiliation:
Graduate Student, Graduate Student, Graduate Student, Graduate Student, Lecturer and Professor, Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai’an 271018, People’s Republic of China
Jinxin Wang*
Affiliation:
Graduate Student, Graduate Student, Graduate Student, Graduate Student, Lecturer and Professor, Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai’an 271018, People’s Republic of China
*
*Corresponding author’s E-mail: wangjx@sdau.edu.cn
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Abstract

Understanding the mechanism of herbicide resistance is fundamental for designing sustainable weed control strategies and exploiting herbicides rationally. Shortawn foxtail is a problem grass weed infesting several important crops in China. The repeated use of acetyl-CoA carboxylase (ACCase)-inhibiting herbicides has resulted in herbicide resistance in this weed. The ACCase gene of resistant individuals of a shortawn foxtail population (JSLS-1) has an Ile-2041-Thr mutation. F2 generation seeds, originated from the same heterozygous plant, were harvested, and two homozygous mutant (JSLS-1RR) and wild (JSLS-1SS) populations for the Ile-2041-Thr mutation were obtained. In whole plants, the JSLS-1RR population conferred high resistance to fenoxaprop and clodinafop, moderate resistance to haloxyfop, low resistance to pinoxaden, and no obvious resistance to clethodim and sethoxydim, compared with JSLS-1SS and a proven susceptible population (HNXY-1). A derived cleaved amplified polymorphic sequence (dCAPS) marker was developed to rapidly detect the rare Ile-2041-Thr mutation in the shortawn foxtail population. This is the first report of the cross-resistance pattern of Ile-2041-Thr mutation, and the robust dCAPS marker could quickly detect this mutation in shortawn foxtail.

Keywords

Clethodimclodinafopfenoxaprophaloxyfoppinoxadensethoxydimshortawn foxtail, Alopecurus aequalis Sobol.ACCasecross-resistancedCAPSgene mutationresistance mechanism
Type
Physiology/Chemistry/Biochemistry
Information
Weed Science , Volume 65 , Issue 2 , March 2017 , pp. 239 - 246
Copyright
© Weed Science Society of America, 2017 

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Footnotes

Associate Editor: Franck E. Dayan, Colorado State University

References

Literature Cited

Beckie, HJ, Tardif, FJ (2012) Herbicide cross resistance in weeds. Crop Prot 35:15–28 Google Scholar
Bi, YL, Liu, WT, Guo, WL, Li, LX, Yuan, GH, Du, L, Wang, JX (2016) Molecular basis of multiple resistance to ACCase- and ALS-inhibiting herbicides in Alopecurus japonicus from China. Pestic Biochem Physiol 126:22–27 CrossRefGoogle ScholarPubMed
Délye, C (2005) Weed resistance to acetyl coenzyme A carboxylase inhibitors: an update. Weed Sci 53:728–746 Google Scholar
Délye, C, Pernin, F, Michel, S (2011) “Universal” PCR assays detecting mutations in acetyl-coenzyme A carboxylase or acetolactate synthase that endow herbicide resistance in grass weeds. Weed Res 51:353–362 CrossRefGoogle Scholar
Délye, C, Zhang, XQ, Chalopin, C, Michel, S, Powles, SB (2003) An isoleucine residue within the carboxyl-transferase domain of multidomain acetyl-coenzyme A carboxylase is a major determinant of sensitivity to aryloxyphenoxypropionate but not to cyclohexanedione inhibitors. Plant Physiol 132:1716–1723 CrossRefGoogle Scholar
Duke, SO (2012) Why have no new herbicide modes of action appeared in recent years? Pest Manag Sci 68:505–512 CrossRefGoogle ScholarPubMed
Guo, WL, Liu, WT, Li, LX, Yuan, GH, Du, L, Wang, JX (2015a) Molecular basis for resistance to fenoxaprop in shortawn foxtail (Alopecurus aequalis) from China. Weed Sci 63:416–424 Google Scholar
Guo, WL, Lv, LL, Zhang, LL, Li, Q, Wu, CX, Lu, XT, Liu, WT, Wang, JX (2016) Herbicides cross resistance of a multiple resistant short-awn foxtail (Alopecurus aequalis Sobol.) population in wheat field. Chil J Agric Res 76:163–169 Google Scholar
Guo, WL, Yuan, GH, Liu, WT, Bi, YL, Du, L, Zhang, C, Li, Q, Wang, JX (2015b) Multiple resistance to ACCase and AHAS-inhibiting herbicides in shortawn foxtail (Alopecurus aequalis Sobol.) from China. Pestic Biochem Physiol 124:66–72 Google Scholar
Han, H, Yu, Q, Purba, E, Li, M, Walsh, M, Friesen, S, Powles, SB (2012) A rare amino acid substitution Ala-122-Tyr in ALS confers high level and broad resistance across ALS-inhibiting herbicides. Pest Manag Sci 68:1164–1170 Google Scholar
Harwood, JL (1988) Fatty acid metabolism. Annu Rev Plant Physiol 39:101–138 Google Scholar
Hashim, S, Hachinohe, M, Matsumoto, H (2010) Cloning and expression analysis of alpha-tubulin genes in shortawn foxtail (Alopecurus aequalis). Weed Sci 58:89–95 Google Scholar
Heap, IM (2016). The International Survey of Herbicide Resistant Weeds. http:// weedscience.org/Summary/MOA.aspx. Accessed: September 8, 2016Google Scholar
Kaundun, SS, Bailly, GC, Dale, RP, Hutchings, SJ, Mclndoe, E (2013a) A novel W1999S mutation and non-target site resistance impact on acetyl-CoA carboxylase inhibiting herbicides to varying degrees in a UK Lolium multiflorum population. Plos One 8:e58012 Google Scholar
Kaundun, SS, Hutchings, SJ, Dale, RP, Mclndoe, E (2013b) Role of a novel I1781T mutation and other mechanisms in conferring resistance to acetyl-CoA carboxylase inhibiting herbicides in a black-grass population. Plos One 8:e69568 CrossRefGoogle Scholar
Konishi, T, Sasaki, Y (1994) Compartmentalization of two forms of acetyl-CoA carboxylase in plants and the origin of their tolerance toward herbicides. Proc Natl Acad Sci USA 91:3598–3601 Google Scholar
Liu, W, Yuan, GH, Du, L, Guo, WL, Li, LX, Bi, YL, Wang, JX (2015) A rare Pro197Glu substitution in acetolactate synthase (ALS) confers broad-spectrum resistance across ALS inhibitors. Pestic Biochem Physiol 117:31–38 Google Scholar
Malone, JM, Boutsalis, P, Baker, J, Preston, C (2014) Distribution of herbicide-resistant acetyl-coenzyme A carboxylase alleles in Lolium rigidum across grain cropping areas of South Australia. Weed Res 54:78–86 Google Scholar
Neff, MM, Turk, E, Kalishman, M (2002) Web-based primer design for single nucleotide polymorphism analysis. Trends Genet 18:613–615 CrossRefGoogle ScholarPubMed
Powles, SB, Yu, Q (2010) Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol 61:317–347 CrossRefGoogle ScholarPubMed
Seefeldt, SS, Jensen, JE, Fuerst, EP (1995) Log-logistic analysis of herbicide dose–response relationships. Weed Technol 9:218–227 CrossRefGoogle Scholar
Shorrosh, BS, Dixon, RA, Ohlrogge, JB (1994) Molecular cloning, characterization, and elicitation of acetyl-CoA carboxylase from alfalfa. Proc Natl Acad Sci USA 91:4323–4327 Google Scholar
Wenger, J, Niderman, T, Mathews, C (2012) Acetyl-CoA carboxylase inhibitors. Pages 447–477 in Kramer W, Schirmer U, Jeschke P & Witschel M eds, Modern Crop Protection Compounds. Weinheim, Germany: Wiley-VCH Google Scholar
Xia, WW, Pan, L, Li, J, Wang, Q, Feng, YJ, Dong, LY (2015) Molecular basis of ALS- and/or ACCase-inhibitor resistance in shortawn foxtail (Alopecurus aequalis Sobol.). Pestic Biochem Physiol 122:76–80 CrossRefGoogle ScholarPubMed
Yu, LPC, Kim, YS, Tong, L (2010) Mechanism for the inhibition of the carboxyltransferase domain of acetyl-coenzyme A carboxylase by pinoxaden. Proc Natl Acad Sci USA 107:22072–22077 CrossRefGoogle ScholarPubMed
Zhang, ZP (2003) Development of chemical weed control and integrated weed management in China. Weed Biol Manag 3:197–203 CrossRefGoogle Scholar