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Occurrence and Molecular Characterization of Acetolactate Synthase (ALS) Inhibitor–Resistant Kochia (Kochia scoparia) in Western Canada

Published online by Cambridge University Press:  20 January 2017

Hugh J. Beckie ,
Suzanne I. Warwick ,
Connie A. Sauder ,
Chris Lozinski  and
Scott Shirriff
Hugh J. Beckie*
Affiliation:
Agriculture and Agri-Food Canada (AAFC), Saskatoon Research Centre, 107 Science Place, Saskatoon, SK S7N 0X2, Canada
Suzanne I. Warwick
Affiliation:
AAFC, Eastern Cereal and Oilseed Research Centre, K.W. Neatby Building, Central Experimental Farm, Ottawa, ON K1A 0C6, Canada
Connie A. Sauder
Affiliation:
AAFC, Eastern Cereal and Oilseed Research Centre, K.W. Neatby Building, Central Experimental Farm, Ottawa, ON K1A 0C6, Canada
Chris Lozinski
Affiliation:
Agriculture and Agri-Food Canada (AAFC), Saskatoon Research Centre, 107 Science Place, Saskatoon, SK S7N 0X2, Canada
Scott Shirriff
Affiliation:
Agriculture and Agri-Food Canada (AAFC), Saskatoon Research Centre, 107 Science Place, Saskatoon, SK S7N 0X2, Canada
*
Corresponding author's E-mail: hugh.beckie@agr.gc.ca
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Abstract

A survey of 109 fields was conducted across western Canada in spring 2007 to determine the extent of ALS-inhibitor and dicamba (synthetic auxin) resistance in kochia. Weed seedlings were collected from fields in three provinces of western Canada and transplanted into the greenhouse. Seeds were harvested from selfed plants, and the F1 progeny were screened for resistance to the ALS-inhibitor mixture thifensulfuron–tribenuron or dicamba. All kochia populations were susceptible to dicamba. ALS inhibitor–resistant kochia was found in 85% of the fields surveyed in western Canada: 80 of 95 fields in Alberta, six of seven fields in Saskatchewan, and all seven fields in Manitoba. For the 93 ALS inhibitor–resistant populations, the mean frequency (±SE) of parental plants classified as resistant was 61 ± 3%. Most of the resistant populations (87%) were heterogeneous and contained both resistant and susceptible individuals. ALS sequence data (Pro197 and Asp376 mutations) and genotyping data (Trp574 mutation) obtained for 87 kochia parental (i.e., field-collected) plants confirmed the presence of all three target-site mutations as well as two mutational combinations (Pro197 + Trp574, Asp376 + Trp574) in resistant individuals.

En primavera de 2007, se llevó al cabo un estudio en 109 campos en el occidente de Canadá para determinar en Kochia scoparia el grado de resistencia a inhibidor de ALS y dicamba (auxina sintética). En campos de tres provincias del occidente de Canadá, se recolectaron plántulas de esta maleza y se trasplantaron en un invernadero. Las semillas producidas por esas plantas fueron cosechadas y la progenie F1 fue evaluada para determinar su resistencia a la mezcla de thifensulfurón y tribenurón (inhibidores de ALS) o dicamba. Todas las poblaciones de K. scoparia fueron susceptibles a dicamba. Kochia resistente al inhibidor ALS se encontró en el 85% de los campos muestreados en el occidente de Canadá: 80 de 95 campos en Alberta, seis de siete en Saskatchewan y todos los siete de Manitoba. Para las 93 poblaciones resistentes al inhibidor ALS, la frecuencia media (+SE) de plantas madres clasificadas como resistentes, fue 61+3%. La mayoría de las poblaciones resistentes (87%) fueron heterogéneas y contenían tanto individuos resistentes como susceptibles. Los datos de la secuencia ALS (mutaciones Pro197 y Asp376) y los del genotipo (mutación Trp574) obtenidos para las 87 plantas madres (i.e., colectadas en el campo), confirmaron la presencia de las mutaciones de tres lugares-objetivo, así como con dos combinaciones mutacionales (Pro197 + Trp574, Asp376 + Trp574) en individuos resistentes.

Keywords

Dicambathifensulfurontribenuronkochia, Kochia scoparia (L.) Schrad. KCHSC, synonymBassia scoparia (L.) A.J. ScottALS-inhibitor resistanceDNA polymorphismsherbicide resistanceTaqMan genotype assaytarget-site mutation
Type
Notes
Information
Weed Technology , Volume 25 , Issue 1 , March 2011 , pp. 170 - 175
Copyright
Copyright © Weed Science Society of America 

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