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The ability of whale haploid spermatogenic cells to induce calcium oscillations and its relevance to oocyte activation

Published online by Cambridge University Press:  01 May 2007

K. Amemiya ,
M. Hirabayashi ,
H. Ishikawa ,
Y. Fukui  and
S. Hochi
K. Amemiya
Affiliation:
Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan.
M. Hirabayashi
Affiliation:
National Institute for Physiological Sciences, Okazaki, Aichi 444–8787, Japan.
H. Ishikawa
Affiliation:
The Institute of Cetacean Research, Chuo-ku, Tokyo 104–0055, Japan.
Y. Fukui
Affiliation:
Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan.
S. Hochi*
Affiliation:
Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan.
*
All correspondence to: Shinichi Hochi, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. Tel: +81 268 21 5350. Fax: +81 268 21 5830. e-mail: shochi@shinshu-u.ac.jp
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Summary

Interspecies microinsemination assay was applied to examine the ability of minke whale haploid spermatogenic cells to induce Ca2+ oscillations and oocyte activation. Populations of round spermatids (RS), early-stage elongating spermatids (e-ES), late-stage elongating spermatids (1-ES) and testicular spermatozoa (TS) were cryopreserved in the presence of 7.5% glycerol on board ship in the Antarctic Ocean. Repetitive increases of intracellular Ca2+ concentration occurred in 0, 65, 81 and 96% of BDF1 mouse oocytes injected with the postthaw RS, e-ES, 1-ES and TS, respectively. A normal pattern of the Ca2+ oscillations was observed in 26–47% of the responding oocytes. Most oocytes that exhibited Ca2+ oscillations, regardless of the oscillation pattern, resumed meiosis (83–94%). These results indicate that whale spermatogenic cells acquire SOAF activity, which is closely related to their Ca2+ oscillation-inducing ability at the relatively early stage of spermiogenesis.

Keywords

Ca2+ oscillationsMinke whaleMouse assaySOAFSpermiogenesis
Type
Research Articles
Information
Zygote , Volume 15 , Issue 2 , May 2007 , pp. 103 - 108
Copyright
Copyright © Cambridge University Press 2007

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