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Article

The bioactivities of the central segment of Zp2 polypeptide

  • Zuowu Cao (a1) (a2), Chuncheng Nie (a2), Yan Xie (a2) and Dongqin Cai (a2) (a3)
Summary

In order to understand the role of the protein zona pellucida 2 in fertilization, an antibody against a central segment of the zona pellucida 2 peptide, segment 190–505 (Z2eH), was prepared. The influence of the antibody on sperm–zona interaction was tested using the sperm–egg binding assay. The effect of the antibody on fertility was evaluated by passive immunization with anti-Z2eH antibody. Immunohistochemical assay showed that an antibody from rabbit reacted specifically with the natural zona pellucida on mouse ovarian sections. Immunofluorescence assay showed that the antibody bound specifically to the zonae pellucidae of the ovulated oocytes and 2-cell embryos after passive immunization. The antibody-treated oocytes bound capacitated sperm as control oocytes, passive immunization did not impede the action of sperm to fertilize the oocyte in vivo. These findings suggest that the central peptide of ZP2 (190–505) is immunogenic and contains zona pellucida-specific epitopes, however the central polypeptide might not be the crucial part from which to construct a functional domain to bind sperm.

Copyright
COPYRIGHT: © Cambridge University Press 2016 
Corresponding author
All correspondence to: Zuowu Cao. Department of Developmental and Regenerative Biology, Jinan University, Guangzhou 510632, China. E-mail: caozuowu@ymail.com
References
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Zygote
  • ISSN: 0967-1994
  • EISSN: 1469-8730
  • URL: /core/journals/zygote
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