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In vitro fertilization efficiency in coral Acropora digitifera

  • Akira Iguchi (a1) (a2) (a3), Masaya Morita (a4), Yuichi Nakajima (a3) (a4), Akira Nishikawa (a1) (a2) and David Miller (a5) (a1) (a2)...
Abstract
Summary

We performed fertilization experiments with Acropora digitifera, which is one of the dominant scleractinian corals in the Ryukyu Archipelago, Japan, to determine optimal conditions for in vitro manipulations. Our result suggests that conspecific fertilization is essentially complete within 30 min under the experimental conditions used in usual fertilization experiments in corals. Previous in vitro experiments (1 × 105–106 sperm/ml, 4–8 h) are likely to have overestimated the efficiency of fertilization of Acropora spp. in the field. Therefore, we suggest that incubation periods shorter than those used to date (i.e. complete exclusion of sperm 1 h after their addition) would be more appropriate for the estimation of fertilization rates in corals.

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Corresponding author
All correspondence to David Miller. ARC Centre of Excellence for Coral Reef Studies, James Cook University, Australia, Townsville, Queensland, 4811, Australia. Tel: +61 747 814473. Fax: +61 747 816078. e-mail: david.miller@jcu.edu.au
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R.C. Babcock , G.D. Bull , P.L. Harrison , A.J. Heyward , J.K. Oliver , C.C. Wallace & B.L. Willis (1986). Synchronous spawning of 105 scleractinian coral species on the Great Barrier Reef. Mar. Biol. 90, 379–94.

P.L. Harrison , R.C. Babcock , G.D. Bull , J.K. Oliver , C.C. Wallace & B.L. Willis (1984). Mass spawning in tropical reef corals. Science 223, 1186–9.

M. Hatta , H. Fukami , W. Wang , M. Omori , K. Shimoike , T. Hayashibara , Y. Ina & T. Sugiyama (1999). Reproductive and genetic evidence for a reticulate evolutionary history of mass spawning corals. Mol. Biol. Evol. 16, 1607–13.

T. Hayashibara , K. Shimoike , T. Kimura , S. Hosaka , A. Heyward , P. Harrison , K. Kudo & M. Omori (1993). Patterns of coral spawning at Akajima Island, Okinawa, Japan. Mar. Ecol. Prog. Ser. 101, 253–62.

A. Iguchi , L.M. Márquez , B. Knack , C. Shinzato , M.J.H. van Oppen , B.L. Willis , K. Hardie , J. Catmull & D.J. Miller (2007). Apparent involvement of a β1 type integrin in coral fertilization. Mar. Biotech. 9, 760–5.

D.R. Levitan & C. Petersen (1995). Sperm limitation in the sea. Trends Ecol. Evol. 10, 228–31.

L.M. Márquez , M.J.H. van Oppen , B.L. Willis & D.J. Miller (2002). Sympatric populations of the highly cross-fertile coral species Acropora hyacinthus and A. cytherea are genetically distinct. Proc. Royal Soc. Ser. B 269, 1289–94.

P. Mercurio , A.P. Negri , K.A. Burns & A.J. Heyward (2004). The ecotoxicology of vegetable versus mineral based lubricating oils: 3. Coral fertilization and adult corals. Env. Pollut. 129, 183–94.

A.P. Negri & A.J. Heyward (2000). Inhibition of fertilization and larval metamorphosis of the coral Acropora millepora (Ehrenberg, 1834) by petroleum products. Mar. Pollut. Bull. 41, 420–7.

A. Negri , C. Vollhardt , C. Humphrey , A. Heyward , R. Jones , G. Eaglesham & K. Fabricius (2005). Effects of the herbicide diuron on the early life history stages of coral. Mar. Pollut. Bull. 51, 370–83.

A.P. Negri , P.A. Marshall & A.J. Heyward (2007). Differing effects of thermal stress on coral fertilization and early embryogenesis in four Indo Pacific species. Coral Reefs 26, 759–63.

J. Oliver & R. Babcock (1992). Aspects of the fertilization ecology of broadcast spawning corals: sperm dilution effects and in situ measurements of fertilization. Biol. Bull. 183, 409–17.

M. Omori , H. Fukami , H. Kobinata & M. Hatta (2001). Significant drop of fertilization of Acropora corals in 1999: an after-effect of heavy coral bleaching? Limnol. Oceanogr. 46, 704–6.

B.L. Willis , R.C. Babcock , P.L. Harrison & C.C. Wallace (1997). Experimental hybridization and breeding incompatibilities within the mating systems of mass spawning reef corals. Coral Reefs 16, Suppl. S5365.

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Zygote
  • ISSN: 0967-1994
  • EISSN: 1469-8730
  • URL: /core/journals/zygote
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