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The effect of pre-maturation culture using phosphodiesterase type 3 inhibitor and insulin, transferrin and selenium on nuclear and cytoplasmic maturation of bovine oocytes

Published online by Cambridge University Press:  30 April 2015

A.L.S. Guimarães ,
S.A. Pereira ,
N.R. Kussano  and
M.A.N. Dode
A.L.S. Guimarães
Affiliation:
School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília-DF, Brazil.
S.A. Pereira
Affiliation:
Institute of Biology, University of Brasília, Brasília-DF, Brazil.
N.R. Kussano
Affiliation:
School of Veterinary Medicine, University of Uberlândia, Uberlândia-MG, Brazil.
M.A.N. Dode*
Affiliation:
Parque Estação Biológica, Final Av. W5/N, Prédio PBI, 70770–900, Brasília-DF, Brazil. School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília-DF, Brazil. Embrapa-Genetic Resources and Biotechnology, Brasília-DF, Brazil.
*
All correspondence to: Margot Alves Nunes Dode. Parque Estação Biológica, Final Av. W5/N, Prédio PBI, 70770–900, Brasília-DF, Brazil. Tel: +55 61 34484659. Fax: +55 61 3340 3658. E-mail: margot.dode@embrapa.br
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Summary

This study aims to evaluate if a pre-maturation culture (PMC) using cilostamide as a meiotic inhibitor in combination with insulin, transferrin and selenium (ITS) for 8 or 24 h increases in vitro embryo production. To evaluate the effects of PMC on embryo development, cleavage rate, blastocyst rate, embryo size and total cell number were determined. When cilostamide (20 μM) was used in PMC for 8 or 24 h, 98% of oocytes were maintained in germinal vesicles. Although the majority of oocytes resumed meiosis after meiotic arrest, the cleavage and blastocyst rates were lower than the control (P < 0.05). When the cilostamide concentration was lowered (10 μM) and oocytes were arrested for 8 h, embryo development was improved (P < 0.05) and was similar (P > 0.05) to the control. The deleterious effect of 20 μM cilostamide treatment for 24 h on a PMC was confirmed by lower cumulus cell viability, determined by trypan blue staining, in that group compared with the other groups. A lower concentration (10 μM) and shorter exposure time (8 h) minimized that effect but did not improve embryo production. More studies should be performed to determine the best concentration and the arresting period to increase oocyte competence and embryo development.

Keywords

Bovine oocyteCilostamideEmbryoMeiotic arrestPre-maturation culture
Type
Research Article
Information
Zygote , Volume 24 , Issue 2 , April 2016 , pp. 219 - 229
Copyright
Copyright © Cambridge University Press 2015 

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