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Glycine and methionine transport by bovine embryos

  • Catherine Guyader-Joly (a1), Chaqué Khatchadourian (a1) and Yves Ménézo (a1)
Summary

As glycine is one of the most concentrated amino acids in the female genital tract, we investigated its uptake by bovine in vitro matured/in vitro fertilised blastocysts in the presence of increasing concentrations of radiolabelled glycine. We also determined methionine uptake by in vitro and in vivo produced embryos. In our study, the hypothesis of more than one site of enzyme activity for glycine substrate was not validated. We determined a Vmax of 23.4fmol/min per embryo and a Km value of 13.3μM. No significant difference was observed either between in vivo and in vitro derived embryos or between grade 1 and grade 2 embryos for methionine uptake. The methionine and glycine uptake of a day 7 bovine was similar to that of a day 4 mouse blastocyst. This is rather low if we consider the relative cell numbers.

Copyright
COPYRIGHT: © Cambridge University Press 1997
Corresponding author
Y. Ménézo, INSA, Unité de Biologie du développement préimplantatoire, Laboratoire de Biologie Appliquée, Bâtiment 406, 20 avenue Albert Einstein, 69621 Villeurbanne Cedex, France. Telephone: +33/04 72 43 83 39. Fax: +33/04 72 43 85 11.
References
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Zygote
  • ISSN: 0967-1994
  • EISSN: 1469-8730
  • URL: /core/journals/zygote
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