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    Kimble, Katelyn M. Dickinson, Sarah E. and Biase, Fernando H. 2018. Extraction of total RNA from single-oocytes and single-cell mRNA sequencing of swine oocytes. BMC Research Notes, Vol. 11, Issue. 1,
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    Liu, Aoxing Wang, Yachun Sahana, Goutam Zhang, Qin Liu, Lin Lund, Mogens Sandø and Su, Guosheng 2017. Genome-wide Association Studies for Female Fertility Traits in Chinese and Nordic Holsteins. Scientific Reports, Vol. 7, Issue. 1,
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    LABRECQUE, RÉMI VIGNEAULT, CHRISTIAN BLONDIN, PATRICK and SIRARD, MARC-ANDRÉ 2013. Gene Expression Analysis of Bovine Oocytes With High Developmental Competence Obtained From FSH-Stimulated Animals. Molecular Reproduction and Development, p. n/a.
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Messenger RNAs in metaphase II oocytes correlate with successful embryo development to the blastocyst stage

  • Fernando Henrique Biase (a1) (a2), Robin Edward Everts (a2), Rosane Oliveira (a2), Weruska Karyna Freitas Santos-Biase (a3), Giovana Krempel Fonseca Merighe (a3), Lawrence Charles Smith (a4), Lúcia Martelli (a1), Harris Lewin (a2) and Flávio Vieira Meirelles (a5)...
Summary

The mRNAs accumulated in oocytes provide support for embryo development until embryo genomic activation. We hypothesized that the maternal mRNA stock present in bovine oocytes is associated with embryo development until the blastocyst stage. To test our hypothesis, we analyzed the transcriptome of the oocyte and correlated the results with the embryo development. Our goal was to identify genes expressed in the oocyte that correlate with its ability to develop to the blastocyst stage. A fraction of oocyte cytoplasm was biopsied using micro-aspiration and stored for further expression analysis. Oocytes were activated chemically, cultured individually and classified according to their capacity to develop in vitro to the blastocyst stage. Microarray analysis was performed on mRNA extracted from the oocyte cytoplasm fractions and correlated with its ability to develop to the blastocyst stage (good quality oocyte) or arrest at the 8–16-cell stage (bad quality oocyte). The expression of 4320 annotated genes was detected in the fractions of cytoplasm that had been collected from oocytes matured in vitro. Gene ontology classification revealed that enriched gene expression of genes was associated with certain biological processes: ‘RNA processing’, ‘translation’ and ‘mRNA metabolic process’. Genes that are important to the molecular functions of ‘RNA binding’ and ‘translation factor activity, RNA binding’ were also enriched in oocytes. We identified 29 genes with differential expression between the two groups of oocytes compared (good versus bad quality). The content of mRNAs expressed in metaphase II oocytes influences the activation of the embryonic genome and enables further develop to the blastocyst stage.

Copyright
COPYRIGHT: © Cambridge University Press 2012 
Corresponding author
All correspondence to: Flávio Meirelles. Departamento de Ciências Básicas, Faculdade de Zootecnia e Engenharia de Alimentos–Universidade de São Paulo, Pirassununga, São Paulo, Brasil. Tel: +55 19 3565 4112; Fax: +55 19 3565 4117. e-mail: meirellf@usp.br
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Zygote
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