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Murine Asb-17 expression during mouse testis development and spermatogenesis

  • Kye-Seong Kim (a1), Myung-Sun Kim (a1), Soo-Kyung Kim (a1) and Kwang-Hyun Baek (a1)
  • DOI:
  • Published online: 01 August 2004

In this study we isolated a murine mAsb-17 from mouse testis by RT-PCR using primers designed based on the sequences from the GenBank database. The sequence analysis showed that mAsb-17 encodes a 295 amino acid polypeptide with a molecular weight of approximately 34 kDa containing two ankyrin repeats and one SOCS box. The amino acid sequence of mASB-17 showed 87.5%, 98.3% and 92.9% identity with that of human, rat and dog, respectively. Interestingly, northern blot analysis showed that mAsb-17 was expressed only in the testis. The expression analysis by RT-PCR for mAsb-17 in mouse indicates that mAsb-17 is expressed from the fourth week after birth to adult, with the highest expression in round spermatids. Both northern blot and RT-PCR analyses suggest that mASB-17 may play essential roles in testis development and spermatogenesis.

Corresponding author
All correspondence to: Kwang-Hyun Baek, PhD, College of Medicine, Pochon CHA University, CHA General Hospital, 605 Yeoksam 1-dong, Kangnam-Gu, Seoul 135-081, Korea. Tel: +82 2 3468 3197. Fax: +82 2 3468 3264. e-mail:
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  • ISSN: 0967-1994
  • EISSN: 1469-8730
  • URL: /core/journals/zygote
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