Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
Cancel
×
×
Home
  • Home
Article
  • Cited by 4
  • Cited by
    Crossref Citations
    Crossref logo
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.
    Barrera, Natalibeth dos Santos Neto, Pedro C. Cuadro, Federico Bosolasco, Diego Mulet, Ana P. Crispo, Martina Menchaca, Alejo and Yu, Yang 2018. Impact of delipidated estrous sheep serum supplementation on in vitro maturation, cryotolerance and endoplasmic reticulum stress gene expression of sheep oocytes. PLOS ONE, Vol. 13, Issue. 6, p. e0198742.
    Schwarz, Kátia R. L. de Castro, Fernanda C. Schefer, Letícia Botigelli, Ramon C. Paschoal, Daniela M. Fernandes, Hugo Leal, Cláudia L. V. and Altomare, Deborah A. 2018. The role of cGMP as a mediator of lipolysis in bovine oocytes and its effects on embryo development and cryopreservation. PLOS ONE, Vol. 13, Issue. 1, p. e0191023.
    Ortiz-Escribano, Nerea Szymańska, Katarzyna Joanna Bol, Melissa Vandenberghe, Lynn Decrock, Elke Van Poucke, Mario Peelman, Luc Van den Abbeel, Etienne Van Soom, Ann and Leybaert, Luc 2017. Blocking connexin channels improves embryo development of vitrified bovine blastocysts†. Biology of Reproduction, Vol. 96, Issue. 2, p. 288.
    Meneghel, Melissa Dall’Acqua, Priscila Chediek Ambrogi, Marcela Leão, Beatriz C.S. Rocha-Frigoni, Nathália A.S. and Mingoti, Gisele Z. 2017. Lipid content and cryotolerance of in vitro-produced bovine embryos treated with forskolin before vitrification. Pesquisa Veterinária Brasileira, Vol. 37, Issue. 4, p. 395.
    ×
  • Get access
    Add to cart €30.00
    Check if you have access via personal or institutional login
    Log in Register

Reduction in cytoplasmic lipid content in bovine embryos cultured in vitro with linoleic acid in semi-defined medium is correlated with increases in cryotolerance

  • Mônica F. Accorsi (a1), Beatriz Caetano da Silva Leão (a1) (a2), Nathália Alves de Souza Rocha-Frigoni (a1) (a2), Silvia Helena Venturoli Perri (a1) and Gisele Zoccal Mingoti (a3) (a2)...
Summary

We examined whether culturing embryos with linoleic acid (LA) in semi-defined medium reduces lipid accumulation and improves cryosurvival after vitrification. Embryos were cultured with LA (100 μM) and a semi-defined medium was used during in vitro culture (IVC), in which the fetal calf serum was substituted by bovine serum albumin (BSA). There was a reduction (P < 0.05) in the embryonic development rate (Control: 25.8% versus LA: 18.5%), but the proposed system was effective in promoting the decrease (P = 0.0130) in the intracellular lipid content (Control: 27.3 ± 0.7 versus LA: 24.6 ± 0.7 arbitrary fluorescence units of embryos stained with the fluorescent dye Nile Red), consequently increasing (P = 0.0490) the embryo survival after 24h of culture post-warming (Control: 50.0% versus LA: 71.7%). The results question the criteria used to evaluate the efficiency of an in vitro production system specifically with relation to the maximum number of blastocysts produced and suggest that might be more appropriate to improve the desired characteristics of embryos generated in accordance with the specific purpose of in vitro embryo production, commercial or scientific. In conclusion, supplying LA to serum-free culture medium was found to adversely affect the rates of embryo development to the blastocyst stage, but significantly reduced embryo lipid accumulation and improved cryopreservation survival.

Copyright
COPYRIGHT: © Cambridge University Press 2015 
Corresponding author
All correspondence to: G.Z. Mingoti, School of Veterinary Medicine, Department of Animal Health, UNESP–Universidade Estadual Paulista, Araçatuba 16050–680, São Paulo, Brazil. Tel: +55 18 3636 1375. Fax: +55 18 3636 1352. E-mail: gmingoti@fmva.unesp.br
References
Hide All
Abe, H., Yamashita, S., Satoh, T. & Hoshi, H. (2002). Accumulation of cytoplasmic lipid droplets in bovine embryos and cryotolerance of embryos developed in different culture systems using serum-free or serum-containing media. Mol. Reprod. Dev. 61, 57–66.
Al Darwich, A., Perreau, C., Petit, M.H., Papillier, P., Dupont, J., Guillaume, D., Mermillod, P. & Guignot, . (2010). Effect of PUFA on embryo cryoresistence, gene expression and AMPKα phosphorylation in IVF-derived bovine embryos. Prostag. Oth. Lipid M. 93, 30–6.
Alasnier, C., Berdeaux, O., Chardigny, J.M. & Sebedio, J.L. (2002). Fatty acid composition and conjugated linoleic acid content the different tissues in rats fed individual conjugated linoleic acid isomers given as triacylglycerols small star, filled. J. Nutr. Biochem. 13, 337–345.
Andreoli, M.F., Gonzalez, M.A., Martinelli, M.I., Mocchiutti, N.O. & Bernal, C.A. (2009). Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in mice. Nutrition 25, 445–452.
Bain, N.T., Madan, P. & Betts, D.H. (2011). The early embryo to intracellular reactive oxygen species is developmentally regulate. Reprod. Fertil. Dev. 23, 561–575.
Baumgard, L.H., Corl, B.A., Dwyer, D.A. & Bauman, D.E. (2000). Identification of the conjugated linoleic acid isomer that inhibits milk fat synthesis. Am. J. Physiol. Reg. I. 278, 179–84.
Baumgard, L.H., Corl, B.A., Dwyer, D.A. & Bauman, D.E. (2002). Effects of conjugates linoleic acids (CLA) on tissue response to homeostatic signals and plasma variables associated with lipid metabolism in lactating dairy cows. J. Anim. Sci. 80, 1285–1293.
Brown, J.M., Halvorsen, Y.D., Lea-Currie, Y.R., Geigerman, C. & Mcintosh, M. (2001). Trans-10cis-12 but not cis-9trans-11 conjugated linoleic acid attenuates lipidogenesis in primary cultures of stromal vascular cells from human adipose tissue. J. Nutr. 131, 2316–2321.
Corrêa, G.A., Rumpf, R., Mundim, T.C.D., Franco, M.M. & Dode, M.A.N. (2008). Oxygen tension during in vitro culture of bovine embryos: effect in production and expression of genes related to oxidative stress. Anim. Reprod. Sci. 104, 132–142.
Dinnyes, A. & Nedambal, T.L. (2009). Cryopreservation of manipulated embryos: tackling the double jeopardy. Reprod. Fertil. Dev. 21, 45–59.
Fagali, N. & Catalá, A. (2008). Antioxidant activity of conjugated linoleic acid isomers, linoleic acid and its methyl ester determined by photoemission and DPPH techniques. Biophys. Chem. 137, 56–62.
Ferreira, C.R., Eberlin, L.S., Hallett, J.E. & Cooks, R.G. (2012). Single oocyte and single embryo lipid analysis by desorption electrospray ionization mass spectrometry. J. Mass Spectrom. 47, 29–33.
Feugang, J.M., Van Langendonckt, A., Sayoud, H., Rees, J.F., Pampfer, S., Moens, A., Dessy, F. & Donnay, I. (2003). Effect of prooxidant agents added at the morula/blastocyst stage on bovine embryo development, cell death and glutathione contente. Zygote 11, 107–118.
Feugang, J.M., De Roover, R., Moens, A., Leonard, S., Dessy, F. & Donnay, I. (2004). Addition of b-mercaptoethanol or trolox at the morula/blastocyst stage improves the quality of bovine blastocysts and prevents induction of apoptosis and degeneration by prooxidants agents. Theriogenology 61, 71–90.
Genicot, G., Leroy, J.L.M.R., Van Soom, A. & Donnay, I. (2005). The use of a fluorescent dye, Nile Red, to evaluate the lipid content of single mammalian oocytes. Theriogenology 63, 1181–1194.
Gervais, R., McFadden, J.W., Lengi, A.J., Corl, B.A. & Chouinard, P.Y. (2009). Effects of intravenous infusion of trans-10, cis-12 18:2 on mammary lipid metabolism in lactating dairy cows. J. Dairy Sci. 92, 5167–5177.
Gomes, A., Fernandes, E. & Lima, J.L.F.C. (2006). Use of fluorescence probes for detection of reactive oxygen species: a review. J. Fluoresc. 16, 119–139.
Gómez, E., Rodríguez, A., Muñoz, M., Caamaño, J.N., Hidalgo, C.O., Morán, E., Facal, N. & Díez, C. (2008). Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification. Theriogenology 69, 1013–1021.
Goto, Y., Noda, Y., Mori, T. & Nakano, M. (1993). Increased generation of reactive oxygen species in embryo cultured in vitro . Free. Radic. Biol. Med. 15, 69–75.
Guérin, P., Mouatassin, S. & Ménézo, Y. (2001). Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum. Reprod. Update 7, 175–189.
Gupta, M.K., Uhm, S.J. & Lee, H.T. (2010). Effect of vitrification and beta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in vitro fertilization. Fertil. Steril. 8, 2602–2607.
Gutiérrez-Adán, A., Lonergan, P., Rizos, D., Ward, F.A., Boland, M.P., Pintado, B. & De La Fuente, J. (2001). Effect of the in vitro culture system on the kinetics of blastocyst development and sex ratio of bovine embryo. Theriogenology 15, 1117–1126.
Haggarty, P., Wood, M., Ferguson, E., Hoad, G., Srikantharajah, A., Milne, E., Hamilton, M. & Bhattacharya, S. (2006). Fatty acid metabolism in human preimplantation embryos. Hum. Reprod. 21, 766–773.
Halliwell, B. & Gutteridge, J.M.C. (1999). Free Radicals in Biology and Medicine 3rd edn. New York: Oxford University Press, 936 pp.
Harvey, A. J. (2007). The role the oxygen in ruminant preimplantation embryo development and metabolism. Anim. Reprod. Sci. 98, 113–128.
Hochi, S., Kimura, K. & Hanada, A. (1999). Effect of linoleic acid-albumin in the culture medium on freezing sensitivity of in vitro-produced bovine morulae. Theriogenology 52, 497–504.
Kim, J.Y., Kinoshita, M., Ohnishi, M. & Fukui, Y. (2001). Lipid and fatty acid analysis of fresh and frozen-thawed immature and in vitro matured bovine oocytes. Reproduction 122, 131–8.
Koba, K., Belury, M.A. & Sugano, M. (2007). Potential health benefits of conjugated trienoic acids. Lipid Technol. 19, 200–3.
Leão, B.C., Rocha-Frigoni, N.A.S., Cabral, E.C., Coelho, M.B., Ferreira, C.R, Eberlin, M.N., Accorsi, M.F., Nogueira, É. & Mingoti, G.Z. (2015). Improved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile. Theriogenology 84, 127–36.
Lim, K.T., Jang, G., Ko, K.H., Lee, W.W., Park, H.J., Kim, J.J., Lee, S.H., Hwang, W.S., Lee, B.C. & Kang, S.K. (2007). Improved in vitro bovine embryo development and increased efficiency in producing viable calves using defined media. Theriogenology 67, 293–302.
Lonergan, P., Rizos, D., Gutierrez-Adan, A., Farin, T. & Boland, M.P. (2003). Oocyte and embryo quality: effect of origin, culture conditions and gene expression patterns. Reprod. Domest. Anim. 38, 259–67.
Loor, J.J. & Herbein, J.H. (1998). Exogenous conjugated linoleic acid isomers reduce bovine milk fat concentration and yield by inhibiting de novo fatty acid synthesis. J. Nutr. 128, 2411–9.
Marei, W.F., Wathes, D.C. & Fouladi-Nashta, A.A. (2012). Differential effects of linoleic and alpha-linolenic fatty acids on spatial and temporal mitochondrial distribution and activity in bovine oocytes. Reprod. Fertil. Dev. 24, 679–90.
Matwee, C., Betts, D.H. & King, W.A. (2000). Apoptosis in the early bovine embryo. Zygote 8, 57–68.
McEvoy, T.G., Coull, G.D., Broadbent, P.J., Hutchinson, J.S.M. & Speake, B.K. (2000). Fatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida. J. Reprod. Fertil. 118, 163–170.
Nagao, Y., Lijima, R. & Saeki, K. (2008). Interaction between embryos and culture conditions during in vitro development of bovine early embryos. Zygote 16, 127–133.
Nedambale, T.L., Du, F., Yang, X. & Tian, X.C. (2006). Higher survival rate of vitrified and thawed in vitro produced bovine blastocyst following culture in defined medium supplemented with beta-mercaptoethanol. Anim. Reprod. Sci. 93, 61–75.
Pariza, M.W., Park, Y. & Cook, M.E. (2001). The biologically active isomers of conjugated linoleic acid. Progr. Lipid Res. 40, 283–298.
Park, Y., Storkson, J.M., Albright, K.J., Liu, W. & Pariza, M.W. (1999). Evidence that the trans-10, cis-12 isomer of conjugated linoleic acid induces body composition changes in mice. Lipids 34, 235–241.
Parrish, J.J., Susko-Parrish, J., Winer, M.A. & First, N.L. (1988). Capacitation of bovine sperm by heparina. Biol. Reprod. 38, 1171–1180.
Paula-Lopes, F.F. & Hansen, P.J. (2002). Heat shock-induced apoptosis in preimplantation bovine embryos is a developmental regulated phenomenon. Biol. Reprod. 66, 1169–77.
Pereira, R.M., Baptista, M.C., Vasques, M.I., Horta, A.E.M., Portugal, P.V., Bessa, R.J.B., Chagas, J., Silva Pereira, M. & Marques, C.C. (2007). Cryo-survival of bovine blastocysts is enhanced by culture with trans-10, cis-12 conjugated linoleic acid (10t 12c CLA). Anim. Reprod. Sci. 98, 293–301.
Pereira, R.M., Carvalhais, I., Pimenta, J., Baptista, M.C., Vasques, M.I., Horta, A.E.M., Santos, I.C., Marques, M.R., Reis, A., Silva Pereira, M. & Marques, C.C. (2008). Biopsied and vitrified bovine embryos viability is improve by trans10, cis12 conjugated linoleic acid supplementation during in vitro embryo culture. Anim. Reprod. Sci. 106, 322–32.
Reis, A., Rooke, J.A., MacCallum, G.J., Staines, M.E., Ewen, M., Lomax, M.A. & McEvoy, T.G. (2003). Consequences of exposure to serum, with or without vitamin E supplementation, in terms of the fatty acid content and viability of bovine blastocysts produced in vitro . Reprod. Fertil. Dev. 15, 275–284.
Reis, A., Maccallum, G.J. & Mcevoy, T.G. (2005). Accumulation and distribution of neural lipid droplets is nom-uniform in ovine blastocysts produced in vitro in either the presence or absence of serum. Reprod. Fertil. Dev. 17, 815–823.
Rizos, D., Fair, T., Papadopoulos, S., Boland, M.P. & Lonergan, P. (2002). Developmental, qualitative, and ultrastructural differences between ovine and bovine embryos produced in vivo or in vitro . Mol. Reprod. Dev. 62, 320–322.
Rizos, D., Gutierrez-Adan, A., Perez-Garnelo, S., De La Fuente, J., Boland, M.P. & Lonergan, P. (2003). Bovine embryo culture in the presence or the absence of serum: implications for blastocyst development, cryotolerance, and messenger RNA expression. Biol. Reprod. 68, 236–43.
Rizos, D., Clemente, M., Bermejo-Alvarez, P., De La Fuente, J., Lonergan, P. & Gutiérrez-Adán, A. (2008). Consequences of in vitro culture conditions on embryos development and quality. Reprod. Domest. Anim. 43, 44–50.
Rybicka, M., Stachowska, E., Gutowska, I., Parczewski, M., Baśkiewicz, M., Machaliński, B., Boroń-Kaczmarska, A. & Chlubek, D. (2011). Comparative effects of conjugated linoleic acid (CLA) and linoleic acid (LA) on the oxidoreduction status in THP-1 macrophages. J. Agric. Food Chem. 27, 4095–103.
Schmitz, G. & Ecker, J. (2008). The opposing effects of n-3 and n-6 fatty acids. Progr. Lipid Res. 47, 147–55.
Sehat, N., Kramer, J.K., Mossoba, M.M., Yurawecz, M.P., Roach, J.A., Eulitz, K., Morehouse, K.M. & Ku, Y. (1998). Identification of conjugated linoleic acid isomers in cheese by gas chromatography, silver ion high performance liquid chromatography and mass spectral reconstructed ion profiles. Comparison of chromatographic elution sequences. Lipids 33, 963–71.
Seidel, G.E. Jr (2006). Modifying oocytes and embryos to improve their cryopreservation. Theriogenology 65, 228–35.
Sturmey, R.G., Reis, A., Leese, H.J. & Mcevoy, T.G. (2009). Role of fatty acids in energy provision during oocyte maturation and early embryo development. Reprod. Domest. Anim. 44, 50–8.
Sudano, M.J., Paschoal, D.M., Rascado, T.S., Magalhães, L.C.O., Crocomo, L.F., Lima-Neto, J.F. & Landim-Alvarenga, F.C. (2011). Lipid content and apoptosis of in vitro-produced bovine embryos as determinants of susceptibility to vitrification. Theriogenology 75, 1211–20.
Ufer, C., Wang, C.C., Borchert, B., Heydeck, D. & Kuh, H. (2010). Redox control in mammalian embryo development. Antioxid. Redox Signal. 13, 833–75.
Vajta, G., Rindom, N., Peura, T.T., Holm, P., Greve, T. & Callesen, H. (1999). The effect of media, serum and temperature on in vitro survival of bovine blastocysts after open pulled straw (OPS) vitrification. Theriogenology 52, 939–48.
Wilding, M., Dale, B., Marino, M., di Matteo, L., Alviggi, C., Pisaturo, M. L., Lombardi, L. & De Placido, G. (2001). Mitochondrial aggregation patterns and activity in human oocytes and preimplantation embryos. Hum. Reprod. 16, 909–17.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Zygote
  • ISSN: 0967-1994
  • EISSN: 1469-8730
  • URL: /core/journals/zygote
Please enter your name
Please enter a valid email address
Who would you like to send this to? *

CAPTCHA *

Skip to the audio challenge
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed

Cancel
Confirm
×