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Migration of Marrow Stromal Cells in Response to Sustained Release of Stromal-Derived Factor-1α from Poly(lactide ethylene oxide fumarate) Hydrogels

Published online by Cambridge University Press:  31 January 2011

Xuezhong He
Affiliation:
hexuez@engr.sc.edu, University of South Carolina, Columbia, South Carolina, United States
Junyu Ma
Affiliation:
ma8@engr.sc.edu, University of South Carolina, Columbia, South Carolina, United States
Esmaiel Jabbari
Affiliation:
jabbari@engr.sc.edu
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Abstract

Stromal derived factor-1α (SDF-1α) is an important chemokine in stem cell trafficking and plays a critical role in the homing, osteogenesis as well as angiogenesis of bone marrow stromal (BMS) cells. The objective of this work was to investigate the release characteristics of SDF-1α from the degradable poly(lactide ethylene oxide fumarate) (PLEOF) hydrogels and to determine the effect of sustained release of SDF-1α on migration of BMS cells. Three PLEOF macromers with PLA content of 6, 9, and 24 by weight were synthesized by condensation polymerization. The cumulative amount of biologically-active SDF-1α released from the PLEOF hydrogels after 3 weeks was between 20-25% of the initial loading and was independent of PLA/PEG ratio in the hydrogel. The migration of BMS cells in response to the time-release SDF-1α from PLEOF hydrogels closely followed the release kinetics of SDF-1α from the hydrogels. Results demonstrate that migration of BMS cells was significantly increased by the sustained release of SDF-1α from PLEOF hydrogels.

Type
Research Article
Copyright
Copyright © Materials Research Society 2010

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References

1 Zhong, R.K., Law, P., Wong, D., Merzouk, A., Salari, H., and Ball, E.D., Small peptide analogs to stromal derived factor-1 enhance chemotactic migration of human and mouse hematopoietic cells. Exp. Hematol., 2004. 32(5): p. 470475.Google Scholar
2 Zhu, W., Boachie-Adjei, O., Rawlins, B.A., Frenkel, B., Boskey, A.L., Ivashkiv, L.B., and Blobel, C.P., A novel regulatory role for stromal-derived factor-1 signaling in bone morphogenic protein-2 osteogenic differentiation of mesenchymal C2C12 cells. J. Biol. Chem., 2007. 282(26): p. 18676–85.Google Scholar
3 Murphy, J.W., Cho, Y., Sachpatzidis, A., Fan, C., Hodsdon, M.E., and Lolis, E., Structural and functional basis of CXCL12 (stromal cell-derived factor-1 alpha) binding to heparin. J. Biol. Chem, 2007. 282(13): p. 10018–27.Google Scholar
4 Huynh, D.P., Nguyen, M.K., Pi, B.S., Kim, M.S., Chae, S.Y., Kang, C.L., Bong, S.K., Kim, S.W., and Lee, D.S., Functionalized injectable hydrogels for controlled insulin delivery. Biomaterials, 2008. 29(16): p. 25272534.Google Scholar
5 Benoit, D.S.W., Collins, S.D., and Anseth, K.S., Multifunctional hydrogels that promote osteogenic human mesenchymal stem cell differentiation through stimulation and sequestering of bone morphogenic protein 2. Adv. Funct. Mater., 2007. 17(13): p. 20852093.Google Scholar
6 Van Tomme, S.R., Storm, G., and Hennink, W.E., In situ gelling hydrogels for pharmaceutical and biomedical applications. Int. J. Pharmaceut., 2008. 355(12): p. 118.Google Scholar
7 Pitarresi, G., Palumbo, F.S., Giammona, G., Casadei, M.A., and Moracci, F.M., Biodegradable hydrogels obtained by photocrosslinking of dextran and polyaspartamide derivatives. Biomaterials, 2003. 24(23): p. 43014313.Google Scholar
8 Yamamoto, M., Tabata, Y., Hong, L., Miyamoto, S., Hashimoto, N., and Ikada, Y., Bone regeneration by transforming growth factor beta 1 released from a biodegradable hydrogel. J. Contr. Rel., 2000. 64(13): p. 133142.Google Scholar
9 He, X. and Jabbari, E., Material properties and cytocompatibility of injectable MMP degradable poly(lactide ethylene oxide fumarate) hydrogel as a carrier for marrow stromal cells. Biomacromolecules, 2007. 8(3): p. 780–92.Google Scholar
10 Sarvestani, A.S., He, X., and Jabbari, E., Effect of osteonectin-derived peptide on the viscoelasticity of hydrogel/apatite nanocomposite scaffolds. Biopolymers, 2007. 85(4): p. 370–8.Google Scholar
11 Sarvestani, A.S., Xu, W.J., He, X.Z., and Jabbari, E., Gelation and degradation characteristics of in situ photo-crosslinked poly(L-lactid-co-ethylene oxide-co-fumarate) hydrogels. Polymer, 2007. 48(2): p. 71137120.Google Scholar
12 Jabbari, E. and He, X., Synthesis and characterization of bioresorbable in situ crosslinkable ultra low molecular weight poly(lactide) macromer. J. Mater. Sci. Mater. Med., 2008. 19(1): p. 311–8.Google Scholar
13 Suggs, L.J., Krishnan, R.S., Garcia, C.A., Peter, S.J., Anderson, J.M., and Mikos, A.G., In vitro and in vivo degradation of poly(propylene fumarate-co-ethylene glycol) hydrogels. J. Biomed. Mater. Res., 1998. 42(2): p. 312320.Google Scholar
14 He, X., Ma, J., and Jabbari, E., Effect of grafting RGD and BMP-2 protein-derived peptides to a hydrogel substrate on osteogenic differentiation of marrow stromal cells. Langmuir, 2008. 24(21): p. 12508–16.Google Scholar
15 Ji, J.F., He, B.P., Dheen, S.T., and Tay, S.S.W., Interactions of chemokines and chemokine receptors mediate the migration of mesenchymal stem cells to the impaired site in the brain after hypoglossal nerve injury. Stem Cells, 2004. 22(3): p. 415427.Google Scholar
16 Abbott, J.D., Huang, Y., Liu, D., Hickey, R., Krause, D.S., and Giordano, F.J., Stromal cellderived factor-1 alpha plays a critical role in stem cell recruitment to the heart after myocardial infarction but is not sufficient to induce homing in the absence of injury. Circulation, 2004. 110(21): p. 33003305.Google Scholar
17 Ji, J.F., Dheen, S.T., Kumar, S.D., He, B.P., and Tay, S.S.W., Expressions of cytokines and chemokines in the dorsal motor nucleus of the vagus nerve after right vagotomy. Mol. Brain Res., 2005. 142(1): p. 4757.Google Scholar
18 Mori, T., Doi, R., Koizumi, M., Toyoda, E., Ito, D., Kami, K., Masui, T., Fujimoto, K., Tamamura, H., Hiramatsu, K., Fujii, N., and Imamura, M., CXCR4 antagonist inhibits stromal cell-derived factor 1-induced migration and invasion of human pancreatic cancer. Mol. Cancer Therapeut., 2004. 3(1): p. 2937.Google Scholar
19 Huang, X.Q., Shen, J.H., Cui, M., Shen, L.L., Luo, X.M., Ling, K., Pei, G., Jiang, H.L., and Chen, K.X., Molecular dynamics simulations on SDF-1 alpha: Binding with CXCR4 receptor. Biophys. J., 2003. 84(1): p. 171184.Google Scholar