Hostname: page-component-8448b6f56d-t5pn6 Total loading time: 0 Render date: 2024-04-23T17:24:04.463Z Has data issue: false hasContentIssue false
  • English
  • Français

Gene expression of regulatory enzymes involved in the intermediate metabolism of sheep subjected to feed restriction

Published online by Cambridge University Press:  09 August 2012

S. van Harten ,
R. Brito ,
A. M. Almeida ,
T. Scanlon ,
T. Kilminster ,
J. Milton ,
J. Greeff ,
C. Oldham  and
L. A. Cardoso
S. van Harten*
Affiliation:
Instituto de Investigação Científica Tropical, Centro de Veterinária e Zootecnia, Faculdade de Medicina Veterinária, Av. da Universidade Técnica, Pólo Universitário da Ajuda, 1300-477 Lisboa, Portugal Centro Interdisciplinar de Investigação em Sanidade Animal, Faculdade de Medicina Veterinária, Av. da Universidade Técnica, Pólo Universitário da Ajuda, 1300-477 Lisboa, Portugal
R. Brito
Affiliation:
Instituto de Investigação Científica Tropical, Centro de Veterinária e Zootecnia, Faculdade de Medicina Veterinária, Av. da Universidade Técnica, Pólo Universitário da Ajuda, 1300-477 Lisboa, Portugal Centro Interdisciplinar de Investigação em Sanidade Animal, Faculdade de Medicina Veterinária, Av. da Universidade Técnica, Pólo Universitário da Ajuda, 1300-477 Lisboa, Portugal
A. M. Almeida
Affiliation:
Instituto de Investigação Científica Tropical, Centro de Veterinária e Zootecnia, Faculdade de Medicina Veterinária, Av. da Universidade Técnica, Pólo Universitário da Ajuda, 1300-477 Lisboa, Portugal Centro Interdisciplinar de Investigação em Sanidade Animal, Faculdade de Medicina Veterinária, Av. da Universidade Técnica, Pólo Universitário da Ajuda, 1300-477 Lisboa, Portugal
T. Scanlon
Affiliation:
Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, 6151 Western Australia, Australia
T. Kilminster
Affiliation:
Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, 6151 Western Australia, Australia
J. Milton
Affiliation:
Faculty of Natural and Agricultural Sciences, UWA Institute of Agriculture M082, University of Western Australia, Crawley 6009, Western Australia, Australia
J. Greeff
Affiliation:
Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, 6151 Western Australia, Australia
C. Oldham
Affiliation:
Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, 6151 Western Australia, Australia
L. A. Cardoso
Affiliation:
Instituto de Investigação Científica Tropical, Centro de Veterinária e Zootecnia, Faculdade de Medicina Veterinária, Av. da Universidade Técnica, Pólo Universitário da Ajuda, 1300-477 Lisboa, Portugal Centro Interdisciplinar de Investigação em Sanidade Animal, Faculdade de Medicina Veterinária, Av. da Universidade Técnica, Pólo Universitário da Ajuda, 1300-477 Lisboa, Portugal
*
E-mail: amsvanharten@fmv.utl.pt
Get access

Abstract

The effect of feed restriction on gene expression of regulatory enzymes of intermediary metabolism was studied in two sheep breeds (Australian Merino and Dorper) subjected to two nutritional treatments: feed restriction (85% of daily maintenance requirements) and control (ad libitum feeding), during 42 days. The experimental animals (ram lambs) were divided into four groups, n = 5 (Australian Merino control (MC), Australian Merino Restriction (MR), Dorper control (DC) and Dorper Restriction (DR)). After the trial, animals were sacrificed and samples were taken from liver tissue to quantify glucose levels and gene expression of relevant intermediary metabolism enzymes (phosphofructokinase (PFK), pyruvate kinase (PK), phosphoenolpyruvate carboxykinase, fructose 1,6-bisphosphatase, glucose-6-phosphatase, glycogen synthase (GS), fatty acid synthase (FAS), glutamate dehydrogenase (GDH) and carbamoyl phosphate synthase (CPS)) through real-time PCR. During the experimental period, the MR animals lost 12.6% in BW compared with 5.3% lost by the Dorper lambs. MC and DC rams gained, respectively, 8.8% and 14% during the same period. Within the Dorper breed, restricted feed animals revealed a significant decrease over controls in the transcription of PFK (1.95-fold) and PK (2.26-fold), both glycolytic enzymes. The gluconeogenesis showed no change in the feed restricted animals of both breeds. DR feed group presented a significant decrease over the homologous Merino sheep group on GS. In both experimental breeds, FAS mRNA expression was decreased in restricted feed groups. GDH expression was decreased only in the DR animals (1.84-fold) indicating a reduced catabolism of amino acids in these animals. Finally, CPS was significantly (P < 0.05) higher in the Dorper sheep, indicating a facilitated urea synthesis in this breed. These results indicate a better adaptation of metabolic intermediate regulatory enzymes and hepatic glucose production of Dorper sheep to feed restriction concurring with the BW results in the experimental groups.

Keywords

Australian MerinoDorperfeed restrictionintermediate metabolismregulatory enzymes
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 3 , March 2013 , pp. 439 - 445
Copyright
Copyright © The Animal Consortium 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Almeida, A 2011. The Damara in the context of Southern Africa fat-tailed sheep breeds. Tropical Animal Health and Production 43, 1427–1441.Google Scholar
Almeida, AM, Schwalbach, LMJ, deWaal, HO, Greyling, JPC, Cardoso, LA 2004. Serum amino acid and myofibrillar protein profiles in Boer goat bucks following undernutrition. Small Ruminant Research 55, 141–147.Google Scholar
Almeida, AM, Campos, A, Francisco, R, van Harten, S, Cardoso, LA, Coelho, AV 2010. Proteomic investigation of the effects of weight loss in the gastrocnemius muscle of wild and NZW rabbits via 2D-electrophoresis and MALDI-TOF MS. Animal Genetics 41, 260–272.Google Scholar
Cerrilla, M, Martínez, G 2002. Starch digestion and glucose metabolism in the ruminant: a review. Interciencia 28, 380–386.Google Scholar
Cloete, SW, Snyman, M, Herselman, M 2000. Productive performance of Dorper sheep. Small Ruminant Research 36, 119–135.Google Scholar
Dhahbi, JM, Mote, PL, Wingo, J, Tillman, JB, Walford, RL, Spindler, SR 1999. Calories and aging alter gene expression for gluconeogenic, glycolytic, and nitrogen-metabolizing enzymes. American Journal of Physiology. Endocrinology and Metabolism 277, E352–E360.Google Scholar
Faverdin, P 1999. The effect of nutrients on feed intake in ruminants. Proceedings of the Nutrition Society 58, 523–531.Google Scholar
Féry, F 1994. Role of hepatic glucose production and glucose uptake in the pathogenesis of fasting hyperglycemia in type 2 diabetes: normalization of glucose kinetics by short-term fasting. Journal of Clinical Endocrinology and Metabolism 78, 536–542.Google Scholar
Feuers, RJ, Duffy, PH, Leakey, JA, Turturro, A, Mittelstaedt, RA, Hart, RW 1989. Effect of chronic caloric restriction on hepatic enzymes of intermediary metabolism in the male Fischer 344 rat. Mechanisms of Ageing and Development 48, 179–189.Google Scholar
Freer, M, Dove, H, Nolan, J 2007. Nutrient requirements of domesticated ruminants. CSIRO Publishing, Collingwood, Vic., Australia.Google Scholar
Hagopian, K, Ramsey, JJ, Weindruch, R 2003. Influence of age and caloric restriction on liver glycolytic enzyme activities and metabolite concentrations in mice. Experimental Gerontology 38, 253–266.Google Scholar
Kim, TS, Freake, HC 1996. High carbohydrate diet and starvation regulate lipogenic mRNA in rats in a tissue-specific manner. Journal of Nutrition 126, 611–617.CrossRefGoogle Scholar
Kozloski, G 2002. Bioquímica dos Ruminantes, 2nd edition. Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.Google Scholar
Nur, T, Sela, I, Webster, NJG, Madar, Z 1995. Starvation and refeeding regulate glycogen synthase gene expression in rat liver at the posttranscriptional level. Journal of Nutrition 125, 2457–2462.Google Scholar
Paulino, M, Sartori, D, Nishida, S 2006. Metabolismo e Transporte de Nutrientes. Apostila de Bases da Fisiologia Animal, Universidade Estadual Paulista (UNESP), Brasil.Google Scholar
Ugochukwu, NH, Figgers, CL 2006. Modulation of the flux patterns in carbohydrate metabolism in the livers of streptozoticin-induced diabetic rats by dietary caloric restriction. Pharmacology Research 54, 172–180.Google Scholar
van Harten, S, Cardoso, LA 2010. Feed restriction and genetic selection on the expression and activity of metabolism regulatory enzymes in rabbits. Animal 4, 1873–1883.Google Scholar
van Harten, S, Almeida, AM, Morais, Z, Schwalbach, LM, Greyling, JP, de Waal, HO, Cardoso, LA 2003. Free fatty acids and fatty acids of triacylglycerols profiles in muscle and plasma of fed and underfed Boer goats. Nutrition Research 23, 1447–1452.Google Scholar
Velez, JC, Donkin, SS 2005. Feed restriction induces pyruvate carboxylase but not phosphoenolpyruvate carboxykinase in dairy cows. Journal of Dairy Science 88, 2938–2948.Google Scholar
Zhao, S, Fernald, RD 2005. Comprehensive algorithm for quantitative real-time polymerase chain reaction. Journal of Computational Biology 12, 1047–1064.Google Scholar
Supplementary material: File

van Harten supplementary material 1

van Harten supplementary material 1

Download van Harten supplementary material 1(File)
File 46.1 KB
Supplementary material: File

van Harten supplementary material 2

van Harten supplementary material 2

Download van Harten supplementary material 2(File)
File 77.8 KB