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Ultrastructure of in vitro oocyte maturation in buffalo (Bubalus bubalis)

Published online by Cambridge University Press:  25 June 2010

Rafael Gianella Mondadori ,
Tiago Rollemberg Santin ,
Andrei Antonioni Guedes Fidelis ,
Khesller Patrícia Olázia Name ,
Juliana Souza da Silva ,
Rodolfo Rumpf  and
Sônia Nair Báo
Rafael Gianella Mondadori*
Affiliation:
Universidade Federal de Pelotas, Instituto de Biologia, Departamento de Morfologia, Av. Duque de Caxias, 250, Bairro Fragata, Pelotas–RS, 96030-002, Brazil.
Tiago Rollemberg Santin
Affiliation:
SQN 202, Bloco F, Apto 101, Brasilia – DF, Brazil.
Andrei Antonioni Guedes Fidelis
Affiliation:
SQS 216, Bloco C, Apto 203, Brasilia – DF, Brazil.
Khesller Patrícia Olázia Name
Affiliation:
Department of Cellular Biology, Institute of Biological Science, University of Brasilia, Brasilia–DF, Brazil.
Juliana Souza da Silva
Affiliation:
Department of Cellular Biology, Institute of Biological Science, University of Brasilia, Brasilia–DF, Brazil.
Rodolfo Rumpf
Affiliation:
Empresa Brasileira de Pequisa Agropecuária–EMBRAPA–CENARGEN, Brasília–DF, Brazil.
Sônia Nair Báo
Affiliation:
Department of Cellular Biology, Institute of Biological Science, University of Brasilia, Brasilia–DF, Brazil.
*
All correspondence to: Rafael Gianella Mondadori. Universidade Federal de Pelotas, Instituto de Biologia, Departamento de Morfologia, Av. Duque de Caxias, 250, Bairro Fragata, Pelotas–RS, 96030-002, Brazil. Tel/Fax: +55 53 3281 1326. e-mail: rmondadori@hotmail.com; rafael.mondadori@ufpel.edu.br.
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Summary

The objective of the present study was to describe ultrastructural changes in the nucleus and cytoplasmic organelles during in vitro maturation (IVM) of buffalo cumulus–oocyte complexes (COCs). The structures were collected by ovum pick-up (OPU). Some COCs, removed from maturation medium at 0, 6, 12, 18 and 24 h, were processed for transmission electron microscopy. The average number of COCs collected by OPU/animal/session was 6.4, and 44% of them were viable. Immature oocytes had a peripherally located nucleus, Golgi complex and mitochondrial clusters, as well as a large number of coalescent lipid vacuoles. After 6 h of IVM, the oocyte nucleus morphology changed from round to a flatter shape, and the granulosa cells (GC) lost most of their contact with zona pellucida (ZP). At 12 h the first polar body was extruded and the aspect of lipid droplet changed to dark, probably denoting lipid oxidation. Cortical granules were clearly visible at 18 h of maturation, always located along the oocyte periphery. At 24 h of IVM the number of cortical granules increased. Ultrastructure studies revealed that: (1) immature oocytes have a high lipid content; (2) the perivitelline space (PS) increases during IVM; (3) Golgi complexes and mitochondrial clusters migrate to oocyte periphery during IVM; (4) 6 h of IVM are enough to lose contact between GC and ZP; (5) the oocyte lipid droplets’ appearance changes between 6 and 12 h of IVM.

Keywords

Embryo productionMurrah buffaloTransmission electron microscopy
Type
Research Article
Information
Zygote , Volume 18 , Issue 4 , November 2010 , pp. 309 - 314
Copyright
Copyright © Cambridge University Press 2010

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