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A rabbit eye model for in vivo transformation of progenetic metacercariae of Clinostomum complanatum into ovigerous adult worms

Published online by Cambridge University Press:  14 December 2012

A. Rizvi ,
Z.A. Zaidi ,
M.M. Alam ,
A. Zafar ,
P.A.A. Shareef ,
M.K. Saifullah ,
M. Saleemuddin  and
S.M.A. Abidi
A. Rizvi
Affiliation:
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India
Z.A. Zaidi
Affiliation:
Department of Ophthalmology, Nizwa Hospital, PO Box 1222, PC 611, Nizwa, Sultanate of Oman
M.M. Alam
Affiliation:
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India
A. Zafar
Affiliation:
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India
P.A.A. Shareef
Affiliation:
Section of Parasitology, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India
M.K. Saifullah
Affiliation:
Section of Parasitology, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India
M. Saleemuddin
Affiliation:
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India
S.M.A. Abidi*
Affiliation:
Section of Parasitology, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India
*
*E-mail: abidilabamu@gmail.com
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Abstract

Clinostomum complanatum is a digenetic trematode that causes yellow grub disease in some fish species and also shows zoonotic potential by sporadically infecting humans. In this study, progenetic metacercariae of C. complanatum were obtained from the fish Trichogaster fasciatus, and were aseptically placed in conjunctival incisions made in the superior and inferior fornices of the eye of rabbits, which served as the experimental hosts. Worms were harvested without necropsy of the host on days 4 and 8 post infection, to observe in vivo transformation of the progenetic metacercariae into ovigerous adult worms. The worms appeared to cause minimal damage to the host although they were tenaciously attached. In vivo maturation was evident by the development of the vitellaria, enlargement of gonads, the presence of a large number of shelled eggs in a distended uterus and ramifications of the intestinal caeca. Obtaining mature ovigerous worms without sacrificing the host clearly gives the rabbit eye model an advantage over those described previously. Due to the relative advantage of the short time required for maturation and the prolific egg production by C. complanatum, it is suggested that this host–parasite system could be used as an excellent model for classroom teaching of trematode biology and to investigate the cues involved in in vivo transformation and host–parasite interactions.

Type
Research Papers
Information
Journal of Helminthology , Volume 88 , Issue 1 , March 2014 , pp. 69 - 73
Copyright
Copyright © Cambridge University Press 2012 

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