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Comparative transcriptome analyses of adzuki bean weevil (Callosobruchus chinensis) response to hypoxia and hypoxia/hypercapnia

Published online by Cambridge University Press:  12 July 2018

S.F. Cui ,
L. Wang ,
L. Ma ,
Y.L. Wang ,
J.P. Qiu ,
Zh.Ch. Liu  and
X.Q. Geng
S.F. Cui
Affiliation:
School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, China Department of Resources and Environment, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
L. Wang
Affiliation:
Department of Resources and Environment, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
L. Ma
Affiliation:
Behavioral & Physiological Ecology (BPE) Group, Groningen Institute for Evolutionary Life Sciences, University of Groningen, Nijenborgh 7, 9747 AG Groningen, Netherlands
Y.L. Wang
Affiliation:
Department of Resources and Environment, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
J.P. Qiu
Affiliation:
Department of Resources and Environment, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
Zh.Ch. Liu*
Affiliation:
Department of Resources and Environment, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
X.Q. Geng*
Affiliation:
Department of Resources and Environment, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
*
*Author for correspondence Phone: 86-21-34208239 and 86-021-34208042 Fax: 86-34205877 and 86-21-34206142 E-mail: zhchl@sjtu.edu.cn and xqgeng@sjtu.edu.cn
*Author for correspondence Phone: 86-21-34208239 and 86-021-34208042 Fax: 86-34205877 and 86-21-34206142 E-mail: zhchl@sjtu.edu.cn and xqgeng@sjtu.edu.cn
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Abstract

Stored product insects show high adaption to hypoxia and hypercapnia, but the underlying mechanism is still unclear. Herein, a comparative transcriptome on 4th adzuki bean weevil (Callosobruchus chinensis) instar larvae was studied to clarify the response mechanisms to hypoxia (HA) and hypoxia/hypercapnia (HHA) using NextSeq500 RNA-Seq. Transcript profiling showed a significant difference in HA or HHA exposure both quantitatively and qualitatively. Compared with control, 631 and 253 genes were significantly changed in HHA and HA, respectively. Comparing HHA with HA, 1135 differentially expressed genes (DEGs) were identified. The addition of hypercapnia made a complex alteration on the hypoxia response of bean weevil transcriptome, carbohydrate, energy, lipid and amino acid metabolism were the most highly enriched pathways for genes significantly changed. In addition, some biological processes that were not significantly enriched but important were also discussed, such as immune system and signal transduction. Most of the DEGs related to metabolism both in HHA and HA were up-regulated, while the DEGs related to the immune system, stress response or signal transduction were significantly down-regulated or suppressed. This research reveals a comparatively full-scale result in adzuki bean weevil hypoxia and hypoxia/hypercapnia tolerance mechanism at transcription level, which might provide new insights into the genomic research of this species.

Keywords

adzuki bean weevilcomparative transcriptomehypoxiahypercapnia
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 109 , Issue 2 , April 2019 , pp. 266 - 277
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Copyright
Copyright © Cambridge University Press 2018 

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