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Assessing genetic diversity in three wild Brachymystax lenok populations using AFLP markers

Published online by Cambridge University Press:  29 January 2010

Wang Di ,
Li Shao-Wu ,
Xu Ge-Feng ,
Liu Yang ,
Mou Zhen-Bo  and
Lu Tong-Yan
Wang Di
Affiliation:
Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China
Li Shao-Wu
Affiliation:
Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China
Xu Ge-Feng
Affiliation:
Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China
Liu Yang
Affiliation:
Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China
Mou Zhen-Bo*
Affiliation:
Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China
Lu Tong-Yan*
Affiliation:
Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China
*
*Corresponding author. E-mail: lutongyan@hotmail.com or mouzhenbo@163.com
*Corresponding author. E-mail: lutongyan@hotmail.com or mouzhenbo@163.com
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Abstract

The genetic diversities of 72 individuals from three wild Lenok populations of Mudanjiang River (MD), Yalujiang River (YL) and Wusulijiang River (WSL) in the northeast of China were analysed using amplified fragment length polymorphism (AFLP) markers. The results showed that 541 polymorphic loci out of 559 were amplified by 12 primer pairs and the percentage of polymorphic loci was 96.78%. Shannon indices for the MD, YL and WSL populations were 0.3988±0.2913, 0.3254±0.3037, 0.2125±0.2862, respectively, and Nei's gene diversity indices were 0.2737±0.2062, 0.2229±0.2129, 0.1446±0.1985, respectively. The average total genetic diversity (Ht) was 0.3512±0.0.0208 and the average genetic diversity within populations (Hs) was 0.2137±0.0152. Among the three populations, the average genetic distance (Dst) was 0.1375 and the gene differentiation coefficient (Gst) was 0.3914. The genetic diversity was 60.85% within populations and 39.15% among populations. The gene flow index (Nm) was 0.7776. The analysis of molecular variance (AMOVA) indicated that the average fixation index (Fst) was 0.55336. The variance was 55.16% within populations and 44.84% among populations. The highest polymorphism ratio was in the MD group and the lowest in the WSL group.

Keywords

LenokAFLPgenetic diversity
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 3 , December 2009 , pp. 241 - 247
Copyright
Copyright © China Agricultural University 2009

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References

Dong, CZ, Liu, YX and Zhang, SC (1997) The population ecology characteristic and resources conservation of Brachymystax lenok in Huma River. Chinese Journal of Fisheries 10: 77–81 (in Chinese).Google Scholar
Gaggiotti, OE and Excoffier, L (2000) A simple method of removing the effect of a bottleneck and unequal population sizes on pairwise genetic distances. Proceedings of Biological Sciences 267: 81–87.CrossRefGoogle ScholarPubMed
Hamrick, JL, Godt, MJW, Murawski, DA and Loveless, MD (1991) Correlations between species traits and allozyme diversity: implications for conservation biology. In: Falk, DA and Holsinger, KE (editors) Genetics and Conservation of Rare Plants. Oxford: Oxford University Press.Google Scholar
Hedrick, PW, Dowling, TE, Minckley, WL, Tibbets, CA, Demarais, BD and Marsh, PC (2000) Establishing a captive broodstock for the endangered bonytail chub (Gila elegans). Journal of Heredity 91: 35–39.CrossRefGoogle ScholarPubMed
Keiper, FJ and McConchie, R (2000) An analysis of genetic variation in natural populations of Sticherus flabellatus [R Br (St John)] using amplified fragment length polymorphism (AFLP) markers. Molecular Ecology 9: 571–581.CrossRefGoogle Scholar
Li, SZ (1984) The discussion of geographic distribution for Chinese Salmonidae fish. Chinese Journal of Zoology 7: 76–79.Google Scholar
Li, WY, Gu, WC and Zhou, SL (2003) AFLP analysis on genetic diversity of Quercus mongolica populations. Scientia Silvae Sinicae 39: 29–36 (in Chinese with English abstract).Google Scholar
Liu, XT, Cao, JY and Zhang, ZZ (2000) Studies on the techniques of domestication in pond and artificial reproduction for the natural Brachymystax lenok individuals. Hebei Fishery 5: 6–9 (in Chinese with English abstract).Google Scholar
Ma, B and Jiang, ZF (2007) Genetic diversity and relationship between two species of Brachymystax in Wusuli River revealed by microsatellites. Journal of Fishery Sciences of China 14: 39–45.Google Scholar
Ma, B, Yin, JS and Li, JP (2005) Comparative studies on morphology and taxonomic position of two species of lenok. Acta Zootaxonomica Sinica 30: 257–260 (in Chinese with English abstract).Google Scholar
Masatoshi, N and Sudhir, K (2002) Molecular Evolution and Phylogenetics pp. 76–142. Beijing: High Education Press.Google Scholar
Mou, ZB, Liu, W and Xu, GF (2006) Study on comparative biology of two species of lenok (Brachymystax lenok) in Ussuri River. Chinese Journal of Fisheries 19: 1–8 (in Chinese with English abstract).Google Scholar
Nei, M (1972) Genetic distance between populations. The American Naturalist 106: 283–292.CrossRefGoogle Scholar
Nei, M (1987) Molecular Evolutionary Genetics pp. 187–192. New York: Columbia University Press.CrossRefGoogle Scholar
Sambrook, J and Russell, DW (2002) Molecular Cloning: A Laboratory Manual 3rd edition. New York: Cold Spring Harbor Laboratory Press.Google Scholar
Song, J, Song, ZB, Yue, BS and Zheng, WJ (2006) Assessing genetic diversity of wild populations of Prenant's schizothoracin, Xchizothorax prenanti, using AFLP markers. Environmental Biology of Fish 77: 79–86.CrossRefGoogle Scholar
Tamura, K, Dudley, J, Nei, M and Kumar, S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software version 4.0. Molecular Biology and Evolution 24: 1596–1599.CrossRefGoogle ScholarPubMed
Thorp, JP (1982) The molecular dock hypothesis: biochemical evolution, genetic differentiation and systematics. Annual Review of Ecology Systematics 13: 139–168.CrossRefGoogle Scholar
Wang, S (1998) China Red Data Book of Endangered Animals. Beijing: Science Press (in Chinese).Google Scholar
Wright, S (1978) Evolution and Genetics of Populations, Vol. 4. Variabhility within and among natural populations. Chicago: University of Chicago Press.Google Scholar
Xia, YZ, Sheng, Y and Chen, YY (2006) DNA sequence variation in the mitochondrial control region of lenok (Brachymystax lenok) populations in China. Biodiversity Science 14: 48–54 (in Chinese with English abstract).CrossRefGoogle Scholar
Xu, GF (2006) The Ovary Development and Oogenesis of Lenok (Brachymystax lenok). Harbin: Northeast Agricultural University.Google Scholar
Yeh, FC and Boyle, TJB (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgian Journal of Botany 129: 157.Google Scholar
Zabeau, M and Vos, P (1993) Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Application no. EP0534858. European Patent Office, Paris.Google Scholar
Zhang, FM and Ge, S (2002) Data analysis in population genetics. I. Analysis of RAPD data with AMOVA. Biodiversity Science 10: 438–444.Google Scholar