Hostname: page-component-8448b6f56d-gtxcr Total loading time: 0 Render date: 2024-04-24T16:22:25.369Z Has data issue: false hasContentIssue false
  • English
  • Français

Aerobic environment ensures viability and anti-oxidant capacity when seeds are wet with negative effect when moist: implications for persistence in the soil

Published online by Cambridge University Press:  28 November 2017

Angelino Carta ,
Stefania Bottega  and
Carmelina Spanò
Angelino Carta*
Affiliation:
Department of Biology, Unit of Botany, University of Pisa, via Derna 1, 56126 Pisa, Italy
Stefania Bottega
Affiliation:
Department of Biology, Unit of Plant Physiology, University of Pisa, via L. Ghini 13, 56126 Pisa, Italy
Carmelina Spanò
Affiliation:
Department of Biology, Unit of Plant Physiology, University of Pisa, via L. Ghini 13, 56126 Pisa, Italy
*
Author for correspondence: Angelino Carta, Email: acarta@biologia.unipi.it
Get access Rights & Permissions [Opens in a new window]

Abstract

Interchangeable effects of temperature, moisture content and oxygen on seed longevity have been mostly examined to estimate seed viability during long-term dry storage, whereas few experiments have studied seed viability under near-natural conditions to evaluate seed persistence in the soil. To this end, we artificially aged seeds of Ranunculus baudotii, a hydrophyte widely distributed in temporary ponds constituting an abundant soil seed bank. Seeds were exposed to controlled ageing at three different relative humidities (RH) under both aerobic and anoxic conditions. Their viability, water content, membrane damage, oxidative stress and anti-oxidant enzymatic defence activity were evaluated. Seed survival was longer at higher relative humidity (97% RH), and lowest at a relative humidity (90% RH) simulating moist but not waterlogged soils. Anoxic conditions showed a protective role on viability at lower moisture contents (70% RH). Seed viability was negatively associated with hydrogen peroxide content and correlated with anti-oxidant enzyme activities, but not with membrane damage. Altogether, these results suggest negative roles for moist soils and anoxia in determining seed persistence in the field, but at higher moisture contents the negative effects of anaerobia diminished. The anti-oxidant system activation, even under unfavourable conditions, might recover seeds once all protective processes can operate, pointing out the plasticity of mechanisms involved in seed loss viability.

Keywords

anti-oxidant enzymeshydrogen peroxidemembrane damageoxygenRanunculus baudotiiseed longevity
Type
Research Papers
Information
Seed Science Research , Volume 28 , Issue 1 , March 2018 , pp. 16 - 23
Check for updates
Copyright
Copyright © Cambridge University Press 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abedi, M, Bartelheimer, M and Poschlod, P (2014) Effects of substrate type, moisture and its interactions on soil seed survival of three Rumex species. Plant and Soil 374, 485–495.CrossRefGoogle Scholar
Aebi, H (1984) Catalase in vitro . Methods in Enzymology 105, 121–125.Google Scholar
Anjum, N, Sharma, P, Gill, SS, Hasanuzzaman, M, Khan, EA, Kachhap, K, Mohamed, AA, Thangavel, P, Devi, GD, Vasudhevan, P, Sofo, A, Khan, NA, Misra, AN, Lukatkin, AS, Singh, HP, Pereira, E and Tuteja, N (2016) Catalase and ascorbate peroxidase-representative H2O2-detoxifying heme enzymes in plants. Environmental Science and Pollution Research 23, 19002–19029.Google Scholar
Bailly, C (2004) Active oxygen species and antioxidants in seed biology. Seed Science Research 14, 93–107.CrossRefGoogle Scholar
Bailly, C, El-Maarouf-Bouteau, H and Corbineau, F (2008) From intracellular signaling networks to cell death, the dual role of reactive oxygen species in seed physiology. Comptes Rendus Biologies 331, 806–814.Google Scholar
Bakker, JP, Poschlod, P, Strykstra, RJ, Bekker, RM and Thompson, K (1996) Seed banks and seed dispersal, important topics in restoration ecology. Acta Botanica Neerlandica 45, 461–490.CrossRefGoogle Scholar
Bass, LN (1979) Physiological and other aspects of seed preservation, p. 156 in Rubenstein, I, Phillips, RL, Green, CE and Gengenbach, BG (eds), The Plant Seed: Development, Preservation and Germination, New York, Academic Press.Google Scholar
Bekker, RM, Knevel, I, Tallowin, JBR, Troost, EM and Bakker, JP (1998a) Soil nutrient input effects on seed longevity, a burial experiment with fen meadow species. Functional Ecology 12, 673–682.Google Scholar
Bekker, RM, Oomes, MJM and Bakker, JP (1998b) The impact of groundwater level on soil seed bank survival. Seed Science Research 8, 399–404.CrossRefGoogle Scholar
Bekker, RM, Bakker, JP, Ozinga, WA and Thompson, K (2003) Seed traits, essential for understanding seed longevity. Aspects of Applied Biology 69, 1–9.Google Scholar
Bela, K, Horváth, E, Gallé, Á, Szabdos, L, Tari, I and Csiszár, J (2015) Plant glutathione peroxidases, emerging role of the antioxidant enzymes in plant development and stress responses. Journal of Plant Physiology 176, 192–201.CrossRefGoogle ScholarPubMed
Benvenuti, S, Macchia, M and Miele, S (2001) Quantitative analysis of emergence of seedlings from buried weed seeds with increasing soil depth. Weed Science 49, 528–535.Google Scholar
Bewley, JD, Bradford, KJ, Hilhorst, HWM and Nonogaki, H (2013) Seeds, Physiology of Development, Germination and Dormancy. New York, Springer.Google Scholar
Bonis, A, Lepart, J and Gillas, P (1995) Seed bank dynamics and coexistence of annual macrophytes in a temporary and variable habitat. Oikos 74, 81–92.Google Scholar
Bossuyt, B and Honnay, O (2008) Can the seed bank be used for ecological restoration? An overview of seed bank characteristics in European communities. Journal of Vegetation Science 19, 875–884.CrossRefGoogle Scholar
Bradford, M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.Google Scholar
Casas, C and Ninot, JM (2007) Soil water regime through contrasting pasture communities in a sub-mediterranean landscape. Journal of Hydrology 335, 98–108.Google Scholar
Carta, A, Bedini, G, Foggi, B and Probert, RJ (2012) Laboratory germination and seed bank storage of Ranunculus peltatus subsp. baudotii seeds from the Tuscan Archipelago. Seed Science and Technology 40, 11–20.CrossRefGoogle Scholar
Ellis, RH and Roberts, EH (1980) Improved equations for the prediction of seed longevity. Annals of Botany 45, 13–30.Google Scholar
Ellis, RH and Hong, TD (2007) Seed longevity-moisture content relationship in hermetic and open storage. Seed Science and Technology 35, 423–431.Google Scholar
Eshdat, Y, Holland, D, Faltin, Z and Ben-Hayyim, G (1997) Plant glutathione peroxidases. Physiologia Plantarum 100, 234–240.Google Scholar
Fessel, SA, Vieira, RD, Pessoa da Cruz, MC, de Paula, RC and Panobianco, M (2006) Electrical conductivity testing of corn seeds as influenced by temperature and period of storage. Pesquisa Agropecuária Brasileira 41, 1551–1559.Google Scholar
Fotouo-M, H, du Toit, ES and Robbertse, PJ (2015) Germination and ultrastructural studies of seed produced by a fast-growing, drought-resistant tree: implications for its domestication and seed storage. AoB Plants 7, 1–12.Google Scholar
Groot, SP, de Groot, L, Kodde, J and van Treuren, R (2015) Prolonging the longevity of ex situ conserved seeds by storage under anoxia. Plant Genetic Resources 13, 18–26.Google Scholar
Guil-Guerrero, JL, García Maroto, FF and Giménez Giménez, A (2001) Fatty acid profiles from forty-nine plant species that are potential new sources of γ-linolenic acid. Journal of the American Oil Chemists’ Society 78, 677–684.CrossRefGoogle Scholar
Hay, FR, Adams, J, Manger, K and Probert, R (2008) The use of non-saturated lithium chloride solutions for experimental control of seed water content. Seed Science and Technology 36, 737–746.Google Scholar
Hay, FR and Timple, S (2016) The longevity of desorbing and adsorbing rice seeds. Seed Science Research 26, 317–331.Google Scholar
Hendry, GAF (1993) Oxygen, free radical processes and seed longevity. Seed Science Research 3, 141–153.CrossRefGoogle Scholar
Hossain, MA, Bhattachrjee, S, Armin, S, Qian, P, Xin, W, Li, HY, Burritt, DJ, Fujita, M and Tran, LS (2015) Hydrogen peroxide priming modulates abiotic oxidative stress tolerance, insights from ROS detoxification and scavenging. Frontiers in Plant Science 6, 420.Google Scholar
Ibrahim, AE, Roberts, EH and Murdoch, AJ (1983) Viability of lettuce seeds II. Survival and oxygen uptake in osmotically controlled storage. Journal of Experimental Botany 34, 631–640.Google Scholar
Jana, S and Choudhuri, MA (1982) Glycolate metabolism of three submerged aquatic angiosperm during aging. Aquatic Botany 12, 345–354.Google Scholar
Kibinza, S, Vinel, D, Cȏme, D, Bailly, C and Corbineau, F (2006) Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Physiologia Plantarum 128, 496–506.CrossRefGoogle Scholar
Kranner, I, Minibayeva, F, Beckett, RP and Seal, CE (2010) What is stress? Concepts, definitions and applications in seed science. New Phytologist 188, 655–673.Google Scholar
Kumar, SPJ, Prasad, SR, Banerjee, R and Thammineni, C (2015) Seed birth to death, dual functions of reactive oxygen species in seed physiology. Annals of Botany 116, 663–668.Google Scholar
Long, RL, Panetta, FD, Steadman, KJ, Probert, R, Bekker, RM, Brooks, S and Adkins, SW (2008) Seed persistence in the field may be predicted by laboratory-controlled aging. Weed Science 56, 523–528.Google Scholar
Long, RL, Steadman, KJ, Panetta, FD and Adkins, SW (2009) Soil type does not affect seed ageing when soil water potential and temperature are controlled. Plant and Soil 320, 131.Google Scholar
Long, RL, Kranner, I, Panetta, FD, Birtic, S, Adkins, SW and Steadman, KJ (2011) Wet–dry cycling extends seed persistence by re-instating antioxidant capacity. Plant and Soil 338, 511–519.Google Scholar
Long, RL, Gorecki, MJ, Renton, M, Scott, JK, Colville, L, Goggin, DE, Commander, LE, Westcott, DA, Cherry, H and Finch-Savage, WE (2015) The ecophysiology of seed persistence, a mechanistic view of the journey to germination or demise. Biological Reviews 90, 31–59.Google Scholar
Morscher, F, Kranner, I, Arc, E, Bailly, C and Roach, T (2015) Glutathione redox state, tocochromanols, fatty acids, antioxidant enzymes and protein carbonylation in sunflower seed embryos associated with after-ripening and ageing. Annals of Botany 116, 669–678.Google Scholar
Murdoch, AJ and Ellis, RH (2000) Dormancy, viability and longevity, pp. 183–214 in Fenner, M (ed), Seeds, the Ecology of Regeneration in Plant Communities. Wallingford, CAB International.Google Scholar
Nakano, Y and Asada, K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell and Physiology 22, 867–880.Google Scholar
Navari-Izzo, F, Meneguzzo, S, Loggini, B, Vazzana, C and Sgherri, CLM (1997) The role of the glutathione system during dehydration of Boea hygroscopica . Physiologia Plantarum 99, 23–30.Google Scholar
Newton, R, Hay, F and Probert, R (2009) Protocol for comparative seed longevity testing. Technical Information Sheet_01, Royal Botanic Gardens Kew, Kew.Google Scholar
Pakeman, RJ, Small, JL and Torvell, L (2012) Edaphic factors influence the longevity of seeds in the soil. Plant Ecology 213, 57–65.Google Scholar
Panetta, FD and Lawes, R (2005) Evaluation of weed eradication programs, the delimitation of extent. Diversity and Distributions 11, 435–442.Google Scholar
Paradiso, A, Caretto, S, Leone, A, Nisi, R and De Gara, L (2016) ROS production and scavenging under anoxia and re-oxygenation in Arabidopsis cells, a balance between redox signaling and impairment. Frontiers in Plant Science 7, 1803.Google Scholar
Probert, RJ, Daws, MI and Hay, FR (2009) Ecological correlates of ex situ seed longevity, a comparative study on 195 species. Annals of Botany 104, 57–69.CrossRefGoogle ScholarPubMed
R Development Core Team (2016) R, A language and environment for statistical computing. R Foundation for Statistical Computing., Vienna., Austria. Available at: https://www.r-project.org/ Google Scholar
Rhazi, L, Grillas, P, Tan Ham, L and El Khyari, D (2001) The seed bank and the between years dynamics of the vegetation of a Mediterranean temporary pool (NW Morocco). Ecologia Mediterranea 27, 69–88.Google Scholar
Roberts, EH and Ellis, RH (1989) Water and seed survival. Annals of Botany 63, 39–52.Google Scholar
Saatkamp, A, Affre, L, Dutoit, T and Poschlod, P (2011) Germination traits explain soil seed persistence across species, the case of Mediterranean annual plants in cereal fields. Annals of Botany 107, 415–426.Google Scholar
Saatkamp, A, Poschlod, P and Venable, DL (2014) The functional role of soil seed banks in natural communities, pp. 263–295 in Gallagher, RS (ed), The Ecology of Regeneration in Plant Communities. Wallingford, CAB International.Google Scholar
Shereena, J and Salim, N (2006) Influence of seed moisture content and leakage on germination and viability in Pisum sativum L. seeds. International Journal of Botany 2, 427–430.Google Scholar
Singh, G and Richa (2016) Viability loss of Dendrocalamus hamiltonii seeds with storage associated with membrane phase behaviour and hormonal analysis. International Journal of Life Science 4, 547–553.Google Scholar
Spanò, C, Bruno, M and Bottega, S (2013) Calystegia soldanella, dune versus laboratory plants to highlight key adaptive physiological traits. Acta Physiologiae Plantarum 35, 1329–1336 Google Scholar
Vertucci, CW and Leopold, AC (1984) Bound water in soybean seed and its relation to respiration and imbibitional damage. Plant Physiology 75, 114–117.Google Scholar
Walters, C, Farrant, JM, Pammenter, NW and Berjak, P (2002) Mechanisms of damage in dry seeds, pp. 263–291 in Black, M and Pritchard, HW (eds), Desiccation and Survival in Plants. Drying without Dying. Wallingford, CAB International.Google Scholar
Walters, C, Wheeler, LM and Grotenhuis, JM (2005) Longevity of seeds stored in a genebank, species characteristics. Seed Science Research 15, 1–20.Google Scholar
Supplementary material: Image

Carta et al supplementary material

Carta et al supplementary material 1

Download Carta et al supplementary material(Image)
Image 210.3 KB