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Evaluation of Angiotensin I-Converting Enzyme (ACE) inhibitory potential of some underutilized indigenous fruits of West Bengal using an in vitro model

Published online by Cambridge University Press:  12 November 2013

Susmita Das  and
Bratati De
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Abstract

Introduction. Angiotensin I-Converting Enzyme (ACE) is a key component in regulation of blood pressure by virtue of the rennin-angiotensin system. ACE converts the inactive decapeptide, angiotensin I, into the potent vasopressor octapeptide, angiotensin II, and inactivates bradykinin, which has a vasodilating action. So, inhibition of ACE has become a major target in control of hypertension. It is well known that the consumption of fruits could provide health benefits by lowering the risk of chronic diseases such as metabolic syndrome diseases including type 2 diabetes and cardiovascular disease. Our current study was focused on investigating the ACE inhibitory property of a few underutilized minor fruits of West Bengal using an in vitro assay. Materials and methods. The potential antihypertensive activity of underutilized indigenous edible fruits of West Bengal, India, was evaluated by their ability to inhibit Angiotensin-Converting Enzyme (ACE). The ACE inhibitory property was assayed using ACE from rabbit lung and hippuryl-histidyl-leucine as the substrate. Nineteen fruit species belonging to 15 families were investigated. The percentage ACE inhibitory activities of these fruits were studied at 20 µg fresh weight of fruit extract per mL. The total phenol content of all these fruits was determined following the standard Folin-Ciocalteu method. Total flavonoid content was also measured. Results. The aqueous fruit extracts of the red variety of Trapa bispinosa, Phoenix sylvestris, Cicca acida, Achras sapota and Averrhoa carambola presented more than 75% ACE inhibition. On the other hand, Punica granatum, Spondias pinnata, Trapa bispinosa (green) and Ziziphus mauritiana showed about 50% inhibition. Aegle marmelos, Annona squamosa, Annona reticulata, Feronia elephantum, Physalis peruviana and Syzygium jambos showed low activity (< 50% inhibition). Discussions. To date there has been no report on Angiotensin I-Converting Enzyme inhibitory activities of these underutilized minor fruits of West Bengal, India. During our study no correlation could be established between the % ACE inhibition and the total phenol or flavonoid content of these fruit extracts. So, it appears that non-phenolic components may also be responsible for ACE inhibitory activity. In our investigation we tried to establish the fact that the consumption of these underutilized minor fruits might have potential in managing cardiovascular diseases.

Keywords

IndiaWest Bengalnatural resourcesfruit treesfruitsenzyme activityIndeBengale occidentalressource naturellearbre fruitierfruitsactivité enzymatiqueIndiaBengala Occidentalrecursos naturalesárboles frutalesfrutasactividad enzimática
Type
Original article
Information
Fruits , Volume 68 , Issue 6 , November 2013 , pp. 499 - 506
Copyright
© 2013 Cirad/EDP Sciences

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References

Gohlke, P., Linz, W., Scholkens, B.A., Kuwer, I., Bartenbach, S., Schnell, A., Unger, T., Angiotensin-converting enzyme inhibition improves cardiac function, Hypertension 23 (1994) 411–418.CrossRefGoogle ScholarPubMed
Hollenberg, N.K., Pharmacologic interrupting the rennin-angiotensin system, Annu. Rev. Pharmacol. Toxicol. 19 (1979) 559–582.CrossRefGoogle Scholar
Ondetti, M.A., Cushman, D.W., Enzyme of the renin-angiotensin system and their inhibitors, Annu. Rev. Biochem. 51 (1982) 283–308.CrossRefGoogle ScholarPubMed
Ukeda, H., Matsuda, H., Kuroda, H., Osajima, K., Matsufuji, H., Osajima, Y., Preparation and separation of angiotensin I converting enzyme inhibitory peptides, Nippon Nogeik. Kaishi 65 (1991) 1223–1228.CrossRefGoogle Scholar
Joshipura, K.J., Hu, F.B., Manson, J.E., Stampfer, M.J., Rimm, E.B., Speizer, F.E., Colditz, G., Ascherio, A., Rosner, B., Spiegelman, D., Willett, W.C., The effect of fruit and vegetable intake on risk for coronary heart disease, Ann. Intern. Med. 132 (2001) 1106–1114.CrossRefGoogle Scholar
Costacou, T., Mayer-Davis, E.J., Nutrition and prevention of type 2 diabetes, Annu. Rev. Nutr. 23 (2003) 147–170.CrossRefGoogle ScholarPubMed
Hung, H.C., Joshipura, K.J., Jiang, R., Hu, F.B., Hunter, D., Smith-Warner, S.A., Colditz, G.A., Rosner, B., Spiegelman, D., Willett, W.C., Fruit and vegetable intake and risk of major chronic disease, J. Natl. Cancer Inst. 96 (2004) 1577–1584.CrossRefGoogle Scholar
Landsberg, L., Molitch, M., Diabetes and hypertension: pathogenesis, prevention and treatment, Clin. Exp. Hypertens. 26 (2004) 621–628.CrossRefGoogle Scholar
Anon., WHO expert committee report, hypertension control, World Health Organ. (WHO) Tech. Rep. Series 862, Geneva, Switz., 1996, 1–83.
Johnston, J.I., Franz, V., Renin-angiotensin system: a dual tissue and hormonal system for cardiovascular control, J. Hypertens. 10 (1992) 13–26.CrossRefGoogle Scholar
Atkinson, A.B., Robertson, J.I.S., Captopril in the treatment of clinical hypertension and cardiac failure, Lancet 2 (1979) 836–839.CrossRefGoogle ScholarPubMed
Suh, H.J., Whang, J.H., Lee, H., Angiotensin I converting enzyme inhibitory peptide from corn gluten hydrolysate, Biotechnol. Letter 21 (1999) 1055–1058.CrossRefGoogle Scholar
Kohama, Y., Matsumoto, S., Oka, H., Teramoto, T., Okabe, M., Mimura, T., Isolation of an ACE-inhibitor from tuna muscle, Biochem. Biophys. Res. Commun. 156 (1988) 332–337.CrossRefGoogle Scholar
Funayama, S., Hikino, H., Hypotensive principles of Diospyros kaki leaves, Chem. Pharm. Bull. 27 (1979) 2865–2868.CrossRefGoogle ScholarPubMed
Kameda, K., Takaku, T., Okuda, H., Kimura, Y., Inhibitory effects of various flavonoids isolated from leaves of persimmon on angiotensin-converting enzyme activity, J. Nat. Prod. 50 (1987) 680–683.CrossRefGoogle ScholarPubMed
Park, P.J., Je, J.Y., Kim, S.K., Free radical scavenging activities of chitooligosaccharides by electron spin resonance spectrometry, J. Agric. Food Chem. 51 (2003) 4624–4627.CrossRefGoogle Scholar
Kang, D.G., Kim, Y.C., Sohn, E.J., Lee, Y.M., Lee, A.S., Yin, M.H., Lee, H.S., Hypotensive effect of butein via the inhibition of angiotensin converting enzyme, Biol. Pharm. Bull. 26 (2003) 1345–1347.CrossRefGoogle ScholarPubMed
Nyman, U., Joshi, P., Madsen, L.B., Pedersen, T.B., Pinstrup, M., Rajasekharan, S., George, V., Pushpangadan, P., Ethnomedical information and in vitro screening for angiotensin-converting enzyme inhibition of plants utilized as traditional medicines in Gujarat, Rajasthan and Kerala, J. Ethnopharmacol. 60 (1998) 247–263.CrossRefGoogle ScholarPubMed
Das, S., Das, S., De, B., In vitro inhibition of key enzymes related to diabetes by the aqueous extracts of some fruits of West Bengal, India, Curr. Nutr. Food Sci. 8 (2012) 19–24.Google Scholar
Cushman, D.W., Cheung., H.S., Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung, Biochem. Pharmacol. 20 (1971) 1637–1648.CrossRefGoogle ScholarPubMed
Sadasivam S., Manikam A., Biochemical methods, Wiley Eastern Lrd., India, 1992.
Kim, D., Jeong, S.W., Lee, C.Y., Antioxidant capacity of phenolic phytochemicals from various cultivars of plums, Food Chem. 81 (2003) 321–326.CrossRefGoogle Scholar
Balasuriya, B.W.N., Rupasinghe, H.P.V., Plant flavonoids as angiotensin converting enzyme inhibitors in regulation of hypertension, Func. Foods Health Dis. 5 (2011) 172–188.Google Scholar
Tossou, C.C., Floquet, A.B., Sinsin, B.A., Relationship between production and consumption of fruit grown on the Allada plateau in southern Benin, Fruits 67 (1) (2012) 3–12.CrossRefGoogle Scholar
Kyung, A.J., Tae, C.S., Daeseok, H., In, H.K., Young, E.K., Chang, H.L., Cardiovascular protective properties of kiwifruit extracts in vitro, Biol. Pharm. Bull. 28 (9) (2005) 1782—1785.Google Scholar
Zahra, F.H., Mostafa, J., Ahmad, T., Mohammad, F., The antihypertensive and vasodilator effects of aqueous extract from Berberis vulgaris fruit on hypertensive rats, Phytother. Res. 19 (3) (2005) 222–225.Google Scholar
Verma, S.K., Jain, V., Katewa, S.S., Blood pressure lowering, fibrinolysis enhancing and antioxidant activities of cardamom (Elettaria cardamomum), Indian J. Biochem. Biophys. 46 (6) (2009) 503–506.Google Scholar
Ayub, M.Y., Norazmir, M.N., Mamot, S., Jeeven, K., Hadijah, H., Anti-hypertensive effect of pink guava (Psidium guajava) puree on spontaneous hypertensive rats, Int. Food Res. J. 17 (2010) 89–96.Google Scholar
Basu, A., Penugonda, K., Pomegranate juice: a heart-healthy fruit juice, Nutr. Rev. 67 (1) (2009) 49–56.CrossRefGoogle ScholarPubMed
Narasimhacharya, A.V.R.L., Rupal, A.V., Prajapati, P.C., Angiotensin Converting Enzyme inhibition by certain fruits: an in vitro study, Curr. Trends Biotechnol. Pharm. 4 (3) (2010) 801–808.Google Scholar
Pinto, M.D.S., Kwon, Y.I., Apostolidis, E., Lajolo, F.M., Genovese, M.I.E., Shetty, K., Functionality of bioactive compounds in Brazilian strawberry (Fragaria × ananassa Duch.) cultivars: Evaluation of hyperglycemia and hypertension potential using in vitro models, J. Agric. Food Chem. 56 (2008) 4386–4392.CrossRefGoogle Scholar
Atato, A., Wala, K., Dourma, M., Bellefontaine, R., Woegan, Y.A, Batawila, K., Akpagana, K., Lianas of Togo bearing edible fruit, Fruits 67 (5) (2012) 353–368.CrossRefGoogle Scholar
Dadjo, C., Assogbadjo, A.E., Fandohan, B., Kakai, R.G., Chakeredza, S., Houehanou, T.D., Damme, P. van, Sinsin, B., Uses and management of black plum (Vitex doniana Sweet) in Southern Benin, Fruits 67 (4) (2012) 239–248.CrossRefGoogle Scholar