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The occurrence of Trichinella species in the cougar Puma concolor couguar from the state of Colorado and other regions of North and South America

Published online by Cambridge University Press:  06 May 2016

M.V. Reichard ,
K. Logan ,
M. Criffield ,
J.E. Thomas ,
J.M. Paritte ,
D.M. Messerly ,
M. Interisano ,
G. Marucci  and
E. Pozio
M.V. Reichard*
Affiliation:
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA
K. Logan
Affiliation:
Mammals Research Colorado Parks and Wildlife, 2300 S. Townsend Ave., Montrose, CO 81401, USA
M. Criffield
Affiliation:
Fish and Wildlife Research Institute, Florida Fish and Wildlife Conservation Commission, 298 Sabal Palm Road, Naples, FL 34114, USA
J.E. Thomas
Affiliation:
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA
J.M. Paritte
Affiliation:
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA
D.M. Messerly
Affiliation:
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA
M. Interisano
Affiliation:
Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Rome, Italy
G. Marucci
Affiliation:
Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Rome, Italy
E. Pozio
Affiliation:
Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Rome, Italy
*
*Fax: 405-744-5275 E-mail: mason.reichard@okstate.edu
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Abstract

Trichinella species are zoonotic nematodes that infect wild carnivores and omnivores throughout the world. We examined the prevalence and species of Trichinella infections in cougars (Puma concolor couguar) from Colorado, United States. Tongues from cougars were examined by pepsin–HCl artificial digestion to detect Trichinella spp. larvae. The species or genotype of individual worms was identified by multiplex polymerase chain reaction (PCR). Trichinella spp. larvae were detected in 17 of 39 cougars (43.6% (28.7–59.5%)). Five of the cougars (12.8%) were infected with T. murrelli, 3 (7.7%) were infected with T. pseudospiralis, and 1 (2.6%) had Trichinella genotype T6. Trichinella spp. larvae from eight cougars were not identified at the species level, due to degraded DNA. The high prevalence of Trichinella spp. in cougars from Colorado and reports of the parasite in other populations of Puma spp. suggest that this large predator is a key mammalian reservoir.

Type
Research Papers
Information
Journal of Helminthology , Volume 91 , Issue 3 , May 2017 , pp. 320 - 325
Copyright
Copyright © Cambridge University Press 2016 

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