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Preliminary Characterization of Organic Nucleator and Framework Macromolecules in Mollusc Shells

Published online by Cambridge University Press:  15 February 2011

Richard Humbert
Affiliation:
Departments of Medicine and Genetics, University of Washington, Seattle, WA
M. Sarikayat
Affiliation:
Department of Materials Science and Engineering, University of Washington, Seattle, WA
I. A. Aksay
Affiliation:
Department of Chemical Engineering, Princeton University, Princeton, NJ
J. W. Crabb
Affiliation:
W. Alton Jones Cell Science Center, Lake Placid, NY.
C. E. Furlong
Affiliation:
Departments of Medicine and Genetics, University of Washington, Seattle, WA
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Abstract

The nacreous (mother-of-pearl) sections of molluscan shells are natural laminates of aragonitic calcium carbonate platelets and an organic matrix. The multi-edged platelets are sub-micrometer thick and surrounded on all sides by 10–20 nm-thick organic matrix that contains several proteins and polysaccharides. Our goal is to identify the components and structure of the organic matrix and determine their function in shell formation and organization. We have extracted organic material from red abalone-Haliotis rufescens, and chambered nautilus-Nautilus pompilius. From the soluble portion, we have partially purified two proteins and determined their amino acid compositions.

Type
Research Article
Copyright
Copyright © Materials Research Society 1994

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References

REFERENCES

1. (i) Sarikaya, M., Gunnison, K. E., Yasrebi, M., I. A. Aksay. Mat. Res. Soc. Symp. Proc. 174, 109(1990); (ii) A. P. Jackson, J. F. Vincent, R. M. Turner, Proc. Roy. Soc. Lond. B234, 415 (1988); (iii) M. Sarikaya and Aksay, I. A., in: Results and problems in cell differentiation in biopolymers, edited by S. Case (Springer Verlag, Amsterdam, 1993) p. 1.Google Scholar
2. Watabe, N., J. Ultractruc. Res. 12, 351 (1965).CrossRefGoogle Scholar
3. Wise, S. W. Jr., Eclogae geol. Helv. 63, 775 (1970).Google Scholar
4. Bevelander, G. and Nakahara, H., Calc. Tiss. Res. 3: 84 (1969); (ii) M. Sarikaya, J. Liu, I. A. Aksay, in Biomimetics: Design and processing of materials, edited by M. Sarikaya and I. A Aksay (American Institute of Physics, New York, 1994) p. 33.Google Scholar
5. Currey, J. D. and Taylor, J. D., J. Zool., Lond. 173, 395 (1974).CrossRefGoogle Scholar
6. Liu, J., Sarikaya, M., and Aksay, I. A., Mat. Res. Soc. Symp. Proc. 255, 9 (1992).CrossRefGoogle Scholar
7. Mutvei, H., Zool. Scripta 7, 287 (1978).Google Scholar
8. Hare, P. E. and Abelson, P. H., Carnegie Inst. Wash. Year Book 64, 223 (1968).Google Scholar
9. Degens, E. T., Spencer, D. W., Parker, R.H., Comp. Biochem. Physiol. 20, 553 (1967).Google Scholar
10. Weiner, S., and Hood, L., Science 190, 987 (1976).CrossRefGoogle Scholar
11. Nakahara, H., Kakei, M., Bevelander, G., Venus 39, 167 (1980).Google Scholar
12. Cariolou, M. A., and Morse, D. E., J. Comp. Physiol. B 157, 717 (1988).CrossRefGoogle Scholar
13. Keith, J., Stockwell, S., Ball, D., Remillard, K., Kaplan, D., Thannhauser, T., Sherwood, R., Comp. Biochem. Physiol. 105B, 487 (1993).Google Scholar
14. Smith, P. K., , P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., Klenk, D. C., Anal. Biochem. 150, 76 (1985).CrossRefGoogle Scholar
15. West, K. A. and Crabb, J. W., in Techniques in Protein Chemistry III., edited by Angeletti, R. H. (Academic Press, San Diego, 1992) p. 233.CrossRefGoogle Scholar
16. Furlong, C. E., Richter, R. W., Chapline, C., Crabb, J. W., Biochemistry 30, 10133 (1991).CrossRefGoogle Scholar
17. Schagger, H. and von Jagow, G., Anal. Biochem. 166, 368 (1987).CrossRefGoogle Scholar
18. Wheeler, A. P., George, J. W., Evans, C. A., Science 212, 1397 (1981).Google Scholar
19. Wheeler, A. P., Rusenko, K. W., George, J. W., Sikes, C. S., Comp. Biochem. Physiol. 87B, 953 (1987).Google Scholar
20. Lyman, J. and Fleming, R. H., J. Mar. Res. 3, 134 (1939).Google Scholar
21. Sikes, C. S. and Wheeler, A. P., Chemtech, 18, 620 (1988).Google Scholar
22. Suess, E., Geochim. Cosmochim. Acta 34, 157 (1970).CrossRefGoogle Scholar
23. Kitano, Y., Bull. Chem. Soc. Japan 35, 1973 (1962).CrossRefGoogle Scholar
24. Berner, R. A., Geochim. Cosmochim. Acta 39, 947 (1975).Google Scholar
25. Furlong, C. E. and Humbert, R., Mat. Res. Soc. Symp. Proc. 255, 435 (1992).Google Scholar