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Larvicidal activity of Bacillus thuringiensis var. israelensis Cry11Aa toxin against Haemonchus contortus

Published online by Cambridge University Press:  24 August 2016

ANA PAULA DE SOUZA STORI DE LARA ,
LUCAS BIGOLIN LORENZON ,
ANA MUÑOZ VIANNA ,
FRANCISCO DENIS SOUZA SANTOS ,
LUCIANO SILVA PINTO ,
MARIA ELISABETH AIRES BERNE  and
FÁBIO PEREIRA LEIVAS LEITE
ANA PAULA DE SOUZA STORI DE LARA
Affiliation:
Departamento de Microbiologia e Parasitologia, UFPel, Pelotas, RS, Brazil
LUCAS BIGOLIN LORENZON
Affiliation:
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil
ANA MUÑOZ VIANNA
Affiliation:
Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas (UFPel)Pelotas, RS, Brazil
FRANCISCO DENIS SOUZA SANTOS
Affiliation:
Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel)Pelotas, RS, Brazil
LUCIANO SILVA PINTO
Affiliation:
Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas (UFPel)Pelotas, RS, Brazil
MARIA ELISABETH AIRES BERNE
Affiliation:
Departamento de Microbiologia e Parasitologia, UFPel, Pelotas, RS, Brazil
FÁBIO PEREIRA LEIVAS LEITE*
Affiliation:
Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas (UFPel)Pelotas, RS, Brazil
*
*Corresponding author: Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Capão do Leão, Caixa, Postal 354, CEP: 96010900, Pelotas, RS, Brazil. E-mail: fabio_leite@ufpel.edu.br; fabio@leivasleite.com.br
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Summary

Effective control of gastrointestinal parasites is necessary in sheep production. The development of anthelmintics resistance is causing the available chemically based anthelmintics to become less effective. Biological control strategies present an alternative to this problem. In the current study, we tested the larvicidal effects of Bacillus thuringiensis var. israelensis Cry11Aa toxin against Haemonchus contortus larvae. Bacterial suspensions [2 × 108 colony-forming units (CFU) g−1 of the feces] of B. thuringiensis var. israelensis and recombinant Escherichia coli expressing Cry11Aa toxin were added to naturally H. contortus egg-contaminated feces. The larvae were quantified, and significant reductions of 62 and 81% (P < 0·001) were, respectively observed, compared with the control group. A 30 mL bacterial suspension (1 × 108 CFU mL−1) of B. thuringiensis var. israelensis and recombinant E. coli expressing Cry11Aa toxin were then orally administered to lambs naturally infected with H. contortus. Twelve hours after administration, feces were collected and submitted to coprocultures. Significant larvae reductions (P < 0·001) of 79 and 90% were observed respectively compared with the control group. The results suggest that the Cry11Aa toxin of B. thuringiensis var. israelensis is a promising new class of biological anthelmintics for treating sheep against H. contortus.

Keywords

biological controlCry11AaBacillus thuringiensis var. israelensisHaemonchus contortus
Type
Research Article
Information
Parasitology , Volume 143 , Issue 12 , October 2016 , pp. 1665 - 1671
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Copyright
Copyright © Cambridge University Press 2016 

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