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Amino- and Carboxy-functionalized Nano- and Microstructured Surfaces for Evaluating the Impact of Non-biological Stimuli on Adhesion, Proliferation and Differentiation of Primary Skin-Cells

Published online by Cambridge University Press:  31 January 2011

Petra J. Kluger ,
Marc Panas ,
Lena Schober ,
Guenter E. M. Tovar ,
Heike Mertsching  and
Kirsten Borchers
Petra J. Kluger
Affiliation:
petra.kluger@igb.fraunhofer.de, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Cell Systems, Stuttgart, Germany
Marc Panas
Affiliation:
marc.panas@igb.fraunhofer.de, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Cell Systems, Stuttgart, Germany
Lena Schober
Affiliation:
lena.schober@igb.fraunhofer.de, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Cell Systems, Stuttgart, Germany
Guenter E. M. Tovar
Affiliation:
guenter.tovar@igb.fraunhofer.de, Stuttgart University, Institute for Interfacial Engineering, Stuttgart, Germany
Heike Mertsching
Affiliation:
heike.mertsching@igb.fraunhofer.de, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Cell Systems, Stuttgart, Germany
Kirsten Borchers
Affiliation:
kirsten.borchers@igb.fraunhofer.de, Fraunhofer IGB, Biomimetic interfaces, Nobelstr. 12, Stuttgart, 70569, Germany, +497119704121, +497119704200
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Abstract

To gain basic insight into the impact of non-biological features on cells’ behaviour, primary skin-cells, keratinocytes and fibroblasts, were cultured on amine-functionalized or carboxy-functionalized planar, nano- or microstructured surfaces. Sintered layers of silica nano- or microparticles were used to fabricate structures in the range of naturally occurring structure-sizes. Organo-chemical functionalization was achieved using organo-functional silanes. Primary human keratinocytes and fibroblasts were isolated from human foreskin and cultivated on the modified interfaces. Both cell-types displayed specific proliferation behaviour, depending on surface topography and chemical functionalization: Keratinocytes showed significantly better proliferation on amino-functionalized surfaces than on carboxy-functionalized surfaces. On amino-functional surfaces decree-topography. Fibroblasts, in contrast, tended to proliferate stronger on carboxylated surfaces. Immunohistological staining proofed that actin and vinculin, which is involved in the formation of focal adhesions, were expressed on all modified surfaces, thus revealing intact cytoskeleton and cell-substrate contacts.

Keywords

biomaterialmicrostructuresurface chemistry
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1187: Symposium KK – Structure-Property Relationships in Biomineralized and Biomimetic Composites , 2009 , 1187-KK05-28
Copyright
Copyright © Materials Research Society 2009

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