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The use of dosed and herbage n-alkanes as markers for the determination of herbage intake

Published online by Cambridge University Press:  27 March 2009

R. W. Mayes ,
C. S. Lamb  and
Patricia M. Colgrove
R. W. Mayes
Affiliation:
Hill Farming Research Organisation, Bush Estate, Penicuik, Midlothian, EH2Q OPY
C. S. Lamb
Affiliation:
Hill Farming Research Organisation, Bush Estate, Penicuik, Midlothian, EH2Q OPY
Patricia M. Colgrove
Affiliation:
Hill Farming Research Organisation, Bush Estate, Penicuik, Midlothian, EH2Q OPY
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Summary

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The recovery in the faeces of the n-alkanes of herbage (odd-chain, C27–C35) and of dosed artificial alkanes (even-chain, C28 and C32) was studied in twelve 4-month-old castrated male lambs. The lambs received three levels of cut, fresh perennial ryegrass or a mixed diet of perennial ryegrass (0·70) and a barley-based concentrate (0·30) (500–900 g D.M./day). C28 and C32 n-alkanes (130 mg each), absorbed onto shredded paper, were given once daily for 17 days to test whether the recoveries of herbage and dosed alkanes were similar to enable their use as markers for determining the herbage intake of grazing sheep. Stearic and palmitic acids (130 mg each) were given with the dosed alkanes to half of the animals with the objective of facilitating emulsification of the dosed alkanes within the digestive tract.

With the exception of C27 n-alkane, the faecal recoveries of all alkanes were unaffected by diet, feeding level or emulsifying agent. Faecal recovery of odd- chain herbage n-alkanes increased with increasing C-chain length. The recovery of the dosed C28 n-alkane was slightly greater than the recoveries of both C27, and C29 n-alkanes of herbage. The recoveries of the dosed C32 n-alkane and the herbage C33-alkane were the same.

The mean herbage intake estimated using C33 and C32 n-alkanes was identical to the actual herbage intake. Other alkane pairs gave slight underestimates of herbage intake ranging from 3·5% for the C28–C29 pair to 7·6% for the C27–C28 pair. No cyclical pattern of n-alkane excretion throughout the day was observed. Examination of daily variations in faecal alkane concentrations indicated that the start of alkane dosing should precede the sampling of faeces by at least 6 days.

These results suggest that accurate estimation of herbage intake in grazing sheep is possible from the simultaneous use of dosed C32 and herbage C33 n-alkanes as markers.

The method may be particularly useful in enabling unbiased estimates of herbage intake to be made in animals receiving supplementary feed.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 107 , Issue 1 , August 1986 , pp. 161 - 170
Copyright
Copyright © Cambridge University Press 1986

References

REFERENCES

Albro, P. W. (1976). Bacterial waxes. In Chemistry and Biochemistry of Natural Waxes (ed. Kolattukudy, P. E.), pp. 419–445. Amsterdam: Elsevier.Google Scholar
Gosden, A. F. & Moselby, G. (1984). Naturally occurring forage hydrocarbons as intake markers. In Welsh Plant Breeding Station, Annual Report 1983, pp. 109–110.Google Scholar
Grace, N. D. & Body, D. R. (1981). The possible use of long chain (C19-C32) fatty acids in herbage as an indigestible faecal marker. Journal of Agricultural Science, Cambridge 97, 743–745.CrossRefGoogle Scholar
Hankin, L. & Kolattukudy, P. E. (1968). Metabolism of a plant wax paraffin (n-nonacosane) by a soil bacterium (Micrococcus cerificans). Journal of General Microbiology 51, 457–463.CrossRefGoogle ScholarPubMed
Hopkins, S. J. & Chibnaix, A. C. (1932). Growth of Aspergillus versicolor on higher paraffins. Biochemical Journal 26, 133–142.CrossRefGoogle ScholarPubMed
Jones, R. A. (1970). An Introduction to Qas-Liquid Chromatography. London and New York: Academic Press.Google Scholar
Kolattukttdy, P. E. (1965). Biosynthesis of wax in Brassica oleracea. Biochemistry 4, 1844–1855.CrossRefGoogle Scholar
Kolatttjkudy, P. E. & Hankin, L. (1966). Metabolism of a plant wax paraffin (n-nonacosane) in the rat. Journal of Nutrition 90, 167–174.CrossRefGoogle Scholar
Langlands, J. P. (1975). Techniques for estimating nutrient intake and its utilisation by the grazing ruminant. In Digestion and Metabolism in the Ruminant (ed. McDonald, I. W. and Warner, A. C. I.), pp. 320–332. Armidale: The University of New England Publishing Unit.Google Scholar
McCarthy, R. D. (1964). Mammalian metabolism of straight-chain saturated hydrocarbons. Biochimica et Biophysica Acta 84, 74–79.Google ScholarPubMed
McKenna, E. J. & Kallio, R. E. (1965). The biology of hydrocarbons. Annual Review of Microbiology 19, 183–205.CrossRefGoogle ScholarPubMed
Mayes, R. W. & Lamb, C. S. (1984). The possible use of n-alkanes in herbage as indigestible faecal markers. Proceedings of the Nutrition Society 43, 39A.Google Scholar
Oró, J., Nooner, D. W. & Wixstrom, S. A. (1965). Paraffinic hydrocarbons in pasture plants. Science 147, 870–873.CrossRefGoogle ScholarPubMed
Savary, P. & Constantin, M. J. (1967). Sur la solubilisation micellaire de l'hexadecane et son passage dans la lymphe thoracique du rat. Biochimica et Biophysica Acta 137, 264–276.CrossRefGoogle Scholar
Tulloch, A. P. (1976). Chemistry of waxes of higher plants. In Chemistry and Biochemistry of Natural Waxes (ed. Kolattukudy, P. E.), pp. 235–287. Amsterdam: Elsevier.Google Scholar
Van Soest, P. J. (1965). Use of detergents in the analysis of fibrous feeds. III. Study of effects of heating and drying on yield of fibre and lignin in forages. Journal of the Association of Official Agricultural Chemists 48, 785–790.Google Scholar