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Seasonal and Morphological Variations of Brown Trout (Salmo trutta f. fario) Kidney Peroxisomes: A Stereological Study

Published online by Cambridge University Press:  21 December 2016

Albina D. Resende ,
Alexandre Lobo-da-Cunha ,
Fernanda Malhão  and
Eduardo Rocha
Albina D. Resende*
Affiliation:
CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Departamento de Ciências, Instituto Universitário de Ciências da Saúde (IUCS), Gandra 4585-116, Paredes, Portugal Histomorphology, Physiopathology and Applied Toxicology Group (PATH), Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), U. Porto, Porto 4050-123, Portugal
Alexandre Lobo-da-Cunha
Affiliation:
Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto (U. Porto), Porto 4099-003, Portugal Histomorphology, Physiopathology and Applied Toxicology Group (PATH), Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), U. Porto, Porto 4050-123, Portugal
Fernanda Malhão
Affiliation:
Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto (U. Porto), Porto 4099-003, Portugal
Eduardo Rocha
Affiliation:
Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto (U. Porto), Porto 4099-003, Portugal Histomorphology, Physiopathology and Applied Toxicology Group (PATH), Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), U. Porto, Porto 4050-123, Portugal
*
*Corresponding author. albina.resende@ipsn.cespu.pt
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Abstract

Literature about fish kidney peroxisomes is scarce. To tackle this caveat, a stereological approach on renal peroxisome morphological parameters was performed for the first time in a fish, establishing correlations with maturation stages as it was previously done in brown trout liver. Three-year-old brown trout males and females were collected at the major seasons of their reproductive cycle. Trunk kidney was fixed and processed for catalase cytochemistry. Classical stereological methods were applied to electromicrographs to quantitate morphological parameters. Different seasonal variation patterns were observed between genders, and between renal proximal tubule segments I and II. In males, peroxisomes from proximal tubule segment II had a relatively higher volume and number in May, being individually bigger in February. Females presented similar trends, though with less marked variations. Overall, males and females did not show exactly the same seasonal patterns for most peroxisomal parameters, and no correlations were found between the latter and the gonado-somatic index (GSI). Hence, and despite the variations, the morphology of renal peroxisomes is not strictly correlated with gonad maturation kinetics, therefore suggesting that kidney peroxisome morphology is not seasonally modulated by sex steroids, like estradiol, as it seems to happen in liver peroxisomes.

Keywords

teleostrenal microbodiesreproductive cyclemorphometryultrastructure
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 6 , December 2016 , pp. 1146 - 1154
Copyright
© Microscopy Society of America 2016 

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