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Phylogeny of Trypanosoma (Megatrypanum) theileri and related trypanosomes reveals lineages of isolates associated with artiodactyl hosts diverging on SSU and ITS ribosomal sequences

Published online by Cambridge University Press:  03 October 2005

A. C. RODRIGUES ,
F. PAIVA ,
M. CAMPANER ,
J. R. STEVENS ,
H. A. NOYES  and
M. M. G. TEIXEIRA
A. C. RODRIGUES
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-900, Brazil
F. PAIVA
Affiliation:
Department of Veterinary Pathology, University of Mato Grosso do Sul, Campo Grande, MS, Brazil
M. CAMPANER
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-900, Brazil
J. R. STEVENS
Affiliation:
School of Biological Sciences, University of Exeter, Exeter, EX4 4PS, UK
H. A. NOYES
Affiliation:
Animal Genetics Laboratory, School of Biological Sciences, University of Liverpool, Liverpool L69 7ZD, UK
M. M. G. TEIXEIRA
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-900, Brazil
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Abstract

SSU ribosomal sequences of trypanosomes from Brazilian cattle and water buffalo were used to infer phylogenetic relationships between non-pathogenic T. theileri and allied species parasitic in artiodactyls. T. theileri trypanosomes from distinct geographical regions in Brazil and from other countries were tightly clustered into the ‘clade T. theileri’ distant from the ‘T. brucei clade’ of pathogenic parasites of artiodactyls, and also distinct from trypanosomes of other mammals. The existence of this monophyletic assemblage (T. theileri clade) composed only by isolates from artiodactyl species justifies the continued recognition of the subgenus T. (Megatrypanum) with T. theileri as its type species. Phylogenies based on SSU and ITS1 ribosomal sequences produced the same branching pattern with isolates from different mammalian hosts clustered in 5 lineages: A, related to water buffalo; B, C and D, to cattle; E, to fallow deer. The pattern of host specificity allied to some congruence between host and parasite phylogenies suggested association of these trypanosomes with their respective hosts. Segregation of cattle isolates into three lineages revealed an overall geographical structure. Moreover, positioning of trypanosomes infecting tabanids in the T. theileri clade is consistent with the role of these flies as important vectors of these trypanosomes.

Keywords

Trypanosoma theileriaMegatrypanumArtiodactylacattlebuffalophylogenyevolutionribosomal genetabanid flies
Type
Research Article
Information
Parasitology , Volume 132 , Issue 2 , February 2006 , pp. 215 - 224
Copyright
2005 Cambridge University Press

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