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Analysis of Ferrous on Ten-Eleven Translocation Activity and Epigenetic Modifications of Early Mouse Embryos by Fluorescence Microscopy

Published online by Cambridge University Press:  07 February 2016

Ming-Hui Zhao ,
Shuang Liang ,
Jing Guo ,
Jeong-Woo Choi ,
Nam-Hyung Kim ,
Wen-Fa Lu  and
Xiang-Shun Cui
Ming-Hui Zhao
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea
Shuang Liang
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea
Jing Guo
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea
Jeong-Woo Choi
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea
Nam-Hyung Kim
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea
Wen-Fa Lu*
Affiliation:
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
Xiang-Shun Cui*
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea
*
*Corresponding authors. wenfa2004@163.com; xscui@cbnu.ac.kr
*Corresponding authors. wenfa2004@163.com; xscui@cbnu.ac.kr
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Abstract

Iron is an essential trace element that plays important roles in the cellular function of all organs and systems. However, the function of Fe(II) in mammalian embryo development is unknown. In this study, we investigated the role of Fe(II) during preimplantation embryo development. Depletion of Fe(II) using thiosemicarbazone-24 (TSC24), a specific Fe(II) chelator, rescued quenching of the Fe(II)-sensitive fluorophore phen green-SK. After in vitro fertilization, TSC24 significantly reduced the cleavage rate as well as blastocyst formation. The hatch rate of blastocysts was also reduced with 1 pM TSC24 treatment (20.25±1.86 versus 42.28±12.96%, p<0.05). Blastocysts were cultured in leukemia inhibitory factor-free mouse embryonic stem cell culture medium with or without TSC24, and those with depleted Fe(II) displayed delayed attachment and lost the ability to induce embryoid body formation. To further explore the mechanism of Fe(II) in embryo development, we assessed the expression of 5-hydroxymethylcytosine (5hmC) and OCT4 in the pronuclear and blastocyst stages, respectively. We observed that Fe(II) reduced 5hmC and OCT4 expression, which could be explained by low ten-eleven translocation (TET) enzyme activity induced by TSC24 treatment. These findings demonstrate that Fe(II) is required for mammalian embryo development and that it facilitates the process via regulation of TET activity.

Keywords

Fe(II)TETDNA methylationembryo
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 2 , April 2016 , pp. 342 - 348
Copyright
© Microscopy Society of America 2016 

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