Detection of antibodies against hepatitis A in blood spots dried on filter paper. Is this a reliable method for epidemiological studies?

A. GIL; A. GONZÁLEZ; R. DAL-RÉ; V. DOMINGUEZ; P. ASTASIO; L. AGUILAR

doi:10.1017/S0950268896007297

Abstract

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Diluted dried blood drops on filter paper were compared with serum samples as a specimen source for qualitative anti-HAV antibody determination by ELISA. A total of 298 serum samples and dried blood drops were collected from a population of healthy adolescents (15·3±1·2 years old). The prevalence of anti-HAV antibody obtained by testing serum samples was 7·7% (95% CI: 4·8–10·1). Compared with serum sampling the sensitivity and specificity of diluted dried blood drops were 91·3 and 99·3%. The positive and negative predictive values were 91·3 and 99·3%, respectively, and the likelihood ratios of positive and negative results were 91 and 0·09. It is proposed that this test represents a reliable procedure for anti-HAV antibody testing.

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Detection of antibodies against hepatitis A in blood spots dried on filter paper. Is this a reliable method for epidemiological studies?

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