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Host-location behavior of the tea green leafhopper Empoasca vitis Göthe (Hemiptera: Cicadellidae): olfactory and visual effects on their orientation

Published online by Cambridge University Press:  25 September 2017

X. Zhang ,
T. Pengsakul ,
M. Tukayo ,
L. Yu ,
W. Fang  and
D. Luo
X. Zhang
Affiliation:
School of Life Sciences, Xiamen University, Xiamen, Fujian, 361102, China
T. Pengsakul
Affiliation:
Faculty of Medical Technology, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand
M. Tukayo
Affiliation:
School of Life Sciences, Xiamen University, Xiamen, Fujian, 361102, China
L. Yu
Affiliation:
School of Life Sciences, Xiamen University, Xiamen, Fujian, 361102, China
W. Fang
Affiliation:
College of the Environment & Ecology, Xiamen University, Xiamen, Fujian, 361102, China
D. Luo*
Affiliation:
School of Life Sciences, Xiamen University, Xiamen, Fujian, 361102, China
*
*Author for correspondence: Tel/Fax: +86-592-2184075 E-mail: dmluo@xmu.edu.cn
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Abstract

The tea green leafhopper, Empoasca vitis Göthe, is one of the most serious pests in tea growing areas. This study investigated the roles played by olfaction and vision in host orientation behavior. The compound eye of E. vitis was found to be a photopic eye; few olfactory sensilla were found on the antennae, while abundant gustatory sensilla were recorded on the mouthparts. Three opsin genes (EV_LWop, EV_UVop, EV_Bop) were isolated and found to be mainly expressed in the compound eye compared with other parts of the body. Immunolocalization indicated that the opsins mainly located in the different regions of rhabdom. The transcription levels of EV_LWop, EV_Bop and EV_UVop were reduced by 77.3, 70.0 and 40.0%, respectively, by RNA interference induced by being fed a special RNA-rich diet for 6 days. The rate of tropism to host color was effectively impaired by 67.6 and 29.5% in the dsEV_LWop and dsEV_Bop treatment groups, but there was no significant change in the dsEV_UVop group. The determination of the cause of the tropism indicated that odors from the host over long distances were unable to attract E. vitis and were only detected when the insects were close to the host. The developed compound eye of E. vitis plays a leading role in host location, and the long-wavelength opsin significantly affects the tropism to host color; the lack of olfactory sensilla results in long-distance odors not being able to be detected until the insect is near to the host-plant. The understanding of these behavioral mechanisms, especially the importance of opsin genes is expected to be useful for pest management.

Keywords

leafhopperopsinssensory organsgene silencing
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 108 , Issue 4 , August 2018 , pp. 423 - 433
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Copyright
Copyright © Cambridge University Press 2017 

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