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Gene expression, oocyte nuclear maturation and developmental competence of bovine oocytes and embryos produced after in vivo and in vitro heat shock

Published online by Cambridge University Press:  28 March 2016

Krishna C. Pavani ,
Erica Baron ,
Pedro Correia ,
Joana Lourenço ,
Bruno Filipe Bettencourt ,
Madalena Sousa  and
Fernando Moreira da Silva
Krishna C. Pavani
Affiliation:
Department of Agrarian Sciences, CITA-A (Research Centre for Agricultural and Environmental Sciences and Technology of the Azores), Animal Reproduction, University of the Azores, Angra do Heroísmo 9700–042, Portugal.
Erica Baron
Affiliation:
Centro Universitário Monte Serrat, Rua Comendador Martins, 52 – Vila Matias, Santos – SP, 11015–530, Brazil.
Pedro Correia
Affiliation:
Department of Agrarian Sciences, CITA-A (Research Centre for Agricultural and Environmental Sciences and Technology of the Azores), Animal Reproduction, University of the Azores, Angra do Heroísmo 9700–042, Portugal.
Joana Lourenço
Affiliation:
Department of Agrarian Sciences, CITA-A (Research Centre for Agricultural and Environmental Sciences and Technology of the Azores), Animal Reproduction, University of the Azores, Angra do Heroísmo 9700–042, Portugal.
Bruno Filipe Bettencourt
Affiliation:
Instituto de Investigação e Inovação em Saúde, Institute for Molecular and Cell Biology (IBMC), University of Porto, Porto, Portugal. Hospital de Santo Espírito da Ilha Terceira, SEEBMO, Angra do Heroísmo, Portugal.
Madalena Sousa
Affiliation:
Department of Agrarian Sciences, CITA-A (Research Centre for Agricultural and Environmental Sciences and Technology of the Azores), Animal Reproduction, University of the Azores, Angra do Heroísmo 9700–042, Portugal.
Fernando Moreira da Silva*
Affiliation:
Departamento de Ciências Agrárias, Secção Reprodução, Campus de Angra do Heroísmo, Universidade dos Açores, Rua Capitão João D'Avila, 9700-042 Angra do Heroísmo, Portugal.
*
All correspondence to: Fernando Moreira da Silva. Departamento de Ciências Agrárias, Secção Reprodução, Campus de Angra do Heroísmo, Universidade dos Açores, Rua Capitão João D'Avila, 9700-042 Angra do Heroísmo, Portugal. Tel: +351 295 402200. Fax: +351 295 402209. E-mail: jsilva@uac.pt
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Summary

Three assays were performed. In assay 1, oocytes harvested during the winter months were subjected to kinetic heat shock by stressing the oocytes at 39.5°C (HS1) or at 40.5°C (HS2) for either 6, 12, 18 or 24 h and then matured at control temperature (38.5°C). The nuclear maturation rates (NMR) of all oocytes were recorded after 24 h. In assay 2, oocytes collected year-round maturated, were implanted via in vitro fertilization (IVF) and developed for 9 days. Gene expression analysis was performed on target genes (Cx43, CDH1, DNMT1, HSPA14) with reference to the two housekeeping genes (GAPDH and SDHA) in embryos. Similarly, in assay 3, genetic analysis was performed on the embryos produced from heat-stressed oocytes (from HS1 and HS2). In assay 1, the duration of heat stress resulted in a significant decline in NMR (P < 0.05) with HS1 for maturated oocytes at 86.4 ± 4.3; 65.5 ± 0.7; 51.3 ± 0.9; 38.1 ± 1.9 and 36.3 ± 0.9, for control, 6 h, 12 h, 18 h and 24 h, respectively. For assays 2 and 3, results demonstrated that DNMT1, Cx43 and HSPA14 were down-regulated in the embryos produced in the warm with respect to the cold months (P < 0.05). A constant up- and down-regulation of DNMT1 and HSPA14 genes were observed in both HS-treated samples. Also, an inconsistent pattern of gene expression was observed in Cx43 and CDH1 genes (P < 0.05). Targeted gene expression was aberrant in embryo development, which can provide evidence on early embryo arrest and slowed embryo development.

Keywords

ApoptosisGene quantificationKinetic heat shockMaternal heat stressNuclear maturation
Type
Research Article
Information
Zygote , Volume 24 , Issue 5 , October 2016 , pp. 748 - 759
Copyright
Copyright © Cambridge University Press 2016 

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