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Fabrication of Microfluidic Devices in Thermoplastic Elastomeric Materials for DNA Detection on Thermal Plastic Substrate

Published online by Cambridge University Press:  31 January 2011

Kebin Li ,
Daniel Brassard ,
François Normandin ,
Caroline Miville-Godin ,
Matthias Geissler ,
Emmanuel Roy  and
Teodor Veres
Kebin Li
Affiliation:
Kebin.Li@imi.cnrc-nrc.gc.ca, National Research Council Canada, Industrial Materials Institute, Boucherville, Canada
Daniel Brassard
Affiliation:
Daniel.Brassard@cnrc-nrc.gc.ca, National Research Council Canada, Industrial Materials Institute, Boucherville, Canada
François Normandin
Affiliation:
Francois.Normandin@cnrc-nrc.gc.ca, National Research Council Canada, Industrial Materials Institute, Boucherville, Canada
Caroline Miville-Godin
Affiliation:
Caroline.Miville-Godin@cnrc-nrc.gc.ca, National Research Council Canada, Industrial Materials Institute, Boucherville, Canada
Matthias Geissler
Affiliation:
Matthias.Geissler@cnrc-nrc.gc.ca, National Research Council Canada, Industrial Materials Institute, Boucherville, Canada
Emmanuel Roy
Affiliation:
Emmanuel.Roy@cnrc-nrc.gc.ca, National Research Council Canada, Industrial Materials Institute, Boucherville, Canada
Teodor Veres
Affiliation:
Teodor.veres@cnrc-nrc.gc.ca, National Research Council Canada, Industrial Materials Institute, Boucherville, Canada
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Abstract

Thermoplastic elastomer (TPE) based microfluidic devices integrated with a microfluidic pumping manifold which consists of 4 electromagnetic valves (EMV) were fabricated. The back and forth shuttling flow and its application in the DNA hybridization process were validated on a thermal plastic Zeonor 1060R substrate. The flow rate can be as fast as 23μl/min when the channel width and the channel height are in 100μm, and 25μm, respectively. The DNA hybridization process is detected by using a fluorescence microscopy. Remarkable DNA hybridization is achieved with the continuous flow of the target DNA at a concentration of 10 nM within the first 1 min by using this device.

Keywords

fluidicshot pressingmicroelectro-mechanical (MEMS)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1222: Symposium DD – Microelectromechanical Systems — Materials and Devices III , 2009 , 1222-DD05-24
Copyright
Copyright © Materials Research Society 2010

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