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Genetic diversity in woad (Isatis tinctoria L.) accessions detected by ISSR markers

Published online by Cambridge University Press:  16 March 2011

Luís Rocha ,
Sandra Martins ,
Valdemar Carnide ,
Fernando Braga  and
Carlos Carvalho
Luís Rocha
Affiliation:
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801Vila Real, Portugal
Sandra Martins
Affiliation:
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801Vila Real, Portugal
Valdemar Carnide
Affiliation:
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801Vila Real, Portugal Centre of Genomics and Biotechnology/Institute for Biotechnology and Bioengineering, University of Trás-os-Montes and Alto Douro, 5001-801Vila Real, Portugal
Fernando Braga
Affiliation:
Centre of Chemistry of Vila Real, University of Trás-os-Montes and Alto Douro, 5001-801Vila Real, Portugal
Carlos Carvalho*
Affiliation:
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801Vila Real, Portugal Centre of Genomics and Biotechnology/Institute for Biotechnology and Bioengineering, University of Trás-os-Montes and Alto Douro, 5001-801Vila Real, Portugal
*
*Corresponding author. E-mail: ccarvalh@utad.pt
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Abstract

Woad (Isatis tinctoria L.) was introduced in Europe in ancient times to produce indigo, a natural blue pigment used mainly for dyestuff. This species was cultivated in Portugal until the beginning of the 20th century, especially in the inner North and South. A set of nine inter-simple sequence repeat (ISSR) markers generated 177 reproducible fragments, of which 171 were polymorphic. The mean number of fragments/accession was 111, ranging between 100 (Portugal-Coimbra) and 124 (Poland). The total polymorphism observed was 0.3272, the average polymorphism was 0.1784 and the gene differentiation between accessions was 0.4546. Polymorphism ranged between 53.8% (Austria) and 73.1% (Belgium). The genetic relationship among woad accessions was obtained with unweighted pair group method with arithmetic mean dendrogram based on a molecular marker, clearly clustering the woad accessions according to their geographic origin. The genetic diversity observed in this collection shows that there is a considerable potential for its improvement and that ISSR could be used to evaluate intra- and inter-accession similarities in I. tinctoria species.

Keywords

genetic relationshipsinter-simple sequence repeat-PCR markersIsatis tinctoria L.
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 210 - 213
Copyright
Copyright © NIAB 2011

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References

Al-Qurainy, F (2010) Application of inter simple sequence repeat (ISSR marker) to detect genotoxic effect of heavy metals on Eruca sativa (L.). African Journal of Biotechnology 9: 467–474.Google Scholar
Andersen, NS, Poulsen, G, Andersen, BA, Kiær, LP, D'Hertefeldt, T, Wilkinson, MJ and Jørgensen, RB (2009) Processes affecting genetic structure and conservation: a case study of wild and cultivatedc Brassica rapa. Genetic Resources and Crop Evolution 56: 189–200.CrossRefGoogle Scholar
Darlington, CD and Wyle, AP (1955) Chromosome Atlas of Flowering Plants. London: George Allen & Unwin Ltd, p. 38.Google Scholar
Gilbert, KG and Cooke, DT (2001) Dyes from plants: past usage, present understanding and potential. Plant Growth Regulation 34: 57–69.CrossRefGoogle Scholar
Gilbert, KG, Garton, S, Karam, MA, Arnold, GM, Karp, A, Edwards, KJ, Cooke, DT and Barker, JHA (2002) A high degree of genetic diversity is revealed in Isatis spp. (dyer's woad) by amplified fragment length polymorphism (AFLP). Theoretical and Applied Genetics 104: 1150–1156.CrossRefGoogle Scholar
Lu, X, Liu, L, Gong, Y, Zhao, L, Song, X and Zhu, X (2009) Cultivar identification and genetic diversity analysis of broccoli and its related species with RAPD and ISSR markers. Scientia Horticulturae 122: 645–648.CrossRefGoogle Scholar
Martín, JP and Sánchez-Yélamo, MD (2000) Genetic relationships among species of the genus Diplotaxis (Brassicaceae) using inter-simple sequence repeat markers. Theoretical and Applied Genetics 101: 1234–1241.CrossRefGoogle Scholar
Oberthur, C, Graf, H and Hamburger, M (2004) The content of indigo precursors in Isatis tinctoria leaves – a comparative study of selected accessions and post-harvest treatments. Phytochemistry 65: 3261–3268.CrossRefGoogle Scholar
Pasqualone, A, Caponio, F and Blanco, A (2001) Inter-simple sequence repeat DNA markers for identification of drupas from different Olea europaea L. cultivars. European Food Research and Technology 213: 240–243.CrossRefGoogle Scholar
Paterson, AH, Tanksley, SD and Sorrells, ME (1991) DNA markers in plant improvement. Advances in Agronomy 46: 39–90.CrossRefGoogle Scholar
Rakoczy-Trojanowska, M and Bolibok, H (2004) Characteristics and a comparison of three classes of microsatellite-based markers and their application in plants. Cellular and Molecular Biology Letters 9: 221–238.Google Scholar
Spataro, G and Negri, V (2008) Adaptability and variation in Isatis tinctoria L.: a new crop for Europe. Euphytica 163: 89–102.CrossRefGoogle Scholar
Spataro, G, Taviani, P and Negri, V (2007) Genetic variation and population structure in a Eurasian collection of Isatis tinctoria L. Genetic Resources and Crop Evolution 54: 573–584.CrossRefGoogle Scholar
Stoker, KG, Cooke, DT and Hill, DJ (1998) Influence of light on natural indigo production from woad (Isatis tinctoria). Plant Growth Regulation 25: 181–185.CrossRefGoogle Scholar
Zietkiewicz, E, Rafalski, A and Labuda, D (1994) Genome fingerprinting by simple sequence repeat (SSR) anchored polymerase chain reaction. Genomics 20: 176–183.CrossRefGoogle ScholarPubMed