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Polyacrylate nanoparticles mediated cytotoxic process in breast cancer 4T1 cell line

Published online by Cambridge University Press:  22 January 2014

Susan S. Barros ,
Jaqueline R. Silva ,
Ricardo B. Azevedo ,
Claure N. Lunardi  and
Anderson J. Gomes
Susan S. Barros
Affiliation:
Laboratory of Nanobiotechnology, University of Brasília, Campus Ceilândia, Centro Metropolitano, Conj. A, Lote 01, Ceilândia, Brasília DF, 72220-900, Brazil.
Jaqueline R. Silva
Affiliation:
Institute of Biology, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília DF, 70910-900, Brazil.
Ricardo B. Azevedo
Affiliation:
Institute of Biology, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília DF, 70910-900, Brazil.
Claure N. Lunardi
Affiliation:
Laboratory of Nanobiotechnology, University of Brasília, Campus Ceilândia, Centro Metropolitano, Conj. A, Lote 01, Ceilândia, Brasília DF, 72220-900, Brazil.
Anderson J. Gomes
Affiliation:
Laboratory of Nanobiotechnology, University of Brasília, Campus Ceilândia, Centro Metropolitano, Conj. A, Lote 01, Ceilândia, Brasília DF, 72220-900, Brazil.
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Abstract

The main objective of this study was to develop a polymeric drug delivery system for tamoxifen (TMX), intended to be injectable Eudragit® nanoparticles (NP) for breast cancer treatment. TMX-Eudragit-NP were characterized in terms of particle size, surface morphology, drug physical state by using photon correlation spectrometry (PCS), scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR). The entrapment efficiency (%EE) and in vitro drug release studies were estimated spectrophotometrically by UV-vis technique. The cell toxicity assay was performed in 4T1 cell line using MTT test. TMX-Eudragit-NP showed a maximum entrapment efficient of 23%. The size measurements were compared with the empty nanoparticles and showed values of TMX-Eudragit-NP = 133 ± 30 nm nm, and empty- NP = 273± 50 nm. The zeta potential of particles was +65 and +38 mV for TMX-Eudragit-NP and empty-NP respectively. FTIR studies did not indicate changes in chemical structure or polymer-drug interaction. The cytotoxicity against the 4T1 cells was affected significantly by the released amount of TMX, while empty-NP exhibit no significant cytotoxicity against mouse breast cancer cells (4T1 cell line).

Keywords

polymerbiomaterialnanostructure
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1625: Symposium I – Multiscale Materials in the Study and Treatment of Cancer , 2014 , mrsf13-1625-i06-19
Copyright
Copyright © Materials Research Society 2014 

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