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PRIME Lab Sample Handling and Data Analysis for Accelerator-Based Biomedical Radiocarbon Analysis

Published online by Cambridge University Press:  18 July 2016

Darren J Hillegonds ,
Rae Record ,
Frank A Rickey ,
Steve Badylak ,
George S Jackson ,
Abby Simmons-Byrd ,
David Elmore  and
Michael E Lipschutz
Darren J Hillegonds
Affiliation:
Purdue University, Department of Chemistry, 1393 Brown Building, West Lafayette, Indiana, 47907, USA. Email: terminus_post_quem@yahoo.com or derwood@purdue.edu.
Rae Record
Affiliation:
Purdue University, Department of Biomedical Engineering, 1296 Potter Center, West Lafayette, Indiana, 47907, USA
Frank A Rickey
Affiliation:
PRIME Lab, Purdue University, Department of Physics, 1396 Physics Building, West Lafayette, Indiana, 47907, USA
Steve Badylak
Affiliation:
Purdue University, Department of Biomedical Engineering, 1296 Potter Center, West Lafayette, Indiana, 47907, USA
George S Jackson
Affiliation:
PRIME Lab, Purdue University, Department of Physics, 1396 Physics Building, West Lafayette, Indiana, 47907, USA
Abby Simmons-Byrd
Affiliation:
Purdue University, Department of Biomedical Engineering, 1296 Potter Center, West Lafayette, Indiana, 47907, USA
David Elmore
Affiliation:
PRIME Lab, Purdue University, Department of Physics, 1396 Physics Building, West Lafayette, Indiana, 47907, USA
Michael E Lipschutz
Affiliation:
Purdue University, Department of Chemistry, 1393 Brown Building, West Lafayette, Indiana, 47907, USA. Email: terminus_post_quem@yahoo.com or derwood@purdue.edu.
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Abstract

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Processing and measurement of 200 biomedical samples has provided an opportunity to better understand the characteristics of accelerator mass spectrometry (AMS) analysis of such samples. We have utilized established procedures (Vogel 1992) and developed new methods for handling various biological samples. We have included secondary standards of known isotope ratio for all assays. A method of determining maximum precision for each unknown sample value is also reported. The presented data are an update of the ongoing radiocarbon AMS biomedical program at Purdue University.

Type
I. Becoming Better
Information
Radiocarbon , Volume 43 , Issue 2A: Proceedings of the 17th International Radiocarbon Conference (Part 1 of 3) , 2001 , pp. 305 - 311
Copyright
Copyright © The Arizona Board of Regents on behalf of the University of Arizona 

References

Jackson, GS, Elmore, D, Rickey, FA, Musameh, SA, Sharma, P, Hillegonds, D, Coury, L, Kissinger, P. 2000. The PRIME Lab biomedical program. Nuclear Instruments and Methods in Physics Research B172:899–903.Google Scholar
Jull, AJT, Donahue, DJ, Hatheway, AL, Linick, TW, Toolin, LJ. 1986. Production of graphite targets by deposition from CO/H2 for precision accelerator 14C measurements. Radiocarbon 28(2A):191–7.Google Scholar
Record, RD, Hillegonds, DJ, Simmons, C, Tullius, R, Rickey, FA, Elmore, D, Badylak, SF. 2001. In vivo degradation of 14C-labeled small intestinal submucosa (SIS) when used for urinary bladder repair. Biomaterials 22:2653–9.Google Scholar
Rickey, FA, Elmore, D, Hillegonds, D, Badylak, S, Record, R, Simmons-Byrd, A. 2000. Regeneration of tissue about an animal-based scaffold: AMS studies of the fate of the scaffold. Nuclear Instruments and Methods in Physics Research B172:904–9.Google Scholar
Turteltaub, KW, Vogel, JS. 1996. Applications of accelerator mass spectrometry in toxicology: a highly sensitive tool for low-level isotope measurements. In: Burlingame, AL, Carr, SA, editors. Mass Spectrometry in the Biological Sciences. New Jersey: Humana Press.Google Scholar
Vogel, JS, Turteltaub, KW. 1992. Biomolecular tracing through accelerator mass spectrometry. Trends in Analytical Chemistry 11(4):142–9.CrossRefGoogle Scholar
Vogel, JS, Turteltaub, KW, Felton, JS, Gledhill, BL, Nelson, DE, Southon, JR, Proctor, ID, Davis, JC. 1990. Application of AMS to the biomedical sciences. Nuclear Instruments and Methods in Physics Research B52:524–30.Google Scholar
Vogel, JS. 1992. Rapid production of graphite without contamination for biomedical AMS. 1992. Radiocarbon 34(3):344–50.Google Scholar
Wilson, AT. 1992. A simple technique for converting CO2 to AMS graphite. 1992. Radiocarbon 34(3):318–20.Google Scholar