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The antifungal activity of the cuticular and internal fatty acid methyl esters and alcohols in Calliphora vomitoria

Published online by Cambridge University Press:  08 April 2013

MAREK GOŁĘBIOWSKI ,
MAGDALENA CERKOWNIAK ,
MAŁGORZATA DAWGUL ,
WOJCIECH KAMYSZ ,
MIECZYSŁAWA I. BOGUŚ  and
PIOTR STEPNOWSKI
MAREK GOŁĘBIOWSKI*
Affiliation:
Faculty of Chemistry, Institute for Environmental and Human Health Protection, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland
MAGDALENA CERKOWNIAK
Affiliation:
Faculty of Chemistry, Institute for Environmental and Human Health Protection, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland
MAŁGORZATA DAWGUL
Affiliation:
Faculty of Pharmacy, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416 Gdańsk, Poland
WOJCIECH KAMYSZ
Affiliation:
Faculty of Pharmacy, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416 Gdańsk, Poland
MIECZYSŁAWA I. BOGUŚ
Affiliation:
Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warszawa, Poland
PIOTR STEPNOWSKI
Affiliation:
Faculty of Chemistry, Institute for Environmental and Human Health Protection, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland
*
*Corresponding author: Faculty of Chemistry, Institute for Environmental and Human Health Protection, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland. Tel: +48 58 5235 398. Fax: +48 58 5235 472. E-mail: goleb@chem.univ.gda.pl
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Summary

The composition of the fatty acid methyl ester (FAME) and alcohol fractions of the cuticular and internal lipids of Calliphora vomitoria larvae, pupae and male/female adults was obtained by separating these two fractions by HPLC–LLSD and analysing them quantitatively using GC–MS. Analysis of the cuticular lipids of the worldwide, medically important ectoparasite C. vomitoria revealed 6 FAMEs with odd-numbered carbon chains from C15:0 to C19:0 in the larvae, while internal lipids contained 9 FAMEs ranging from C15:1 to C19:0. Seven FAMEs from C15:0 to C19:0 were identified in the cuticular lipids of the pupae, whereas the internal lipids of the pupae contained 10 FAMEs from C13:0 to C19:0. The cuticular lipids of males and females and also the internal lipids of males contained 5, 7 and 6 FAMEs from C15:0 to C19:0 respectively. Seven FAMEs from C13:0 to C19:0 were identified in the internal lipids of females, and 7, 6, 5 and 3 alcohols were found in the cuticular lipids of larvae, pupae, males and females respectively. Only saturated alcohols with even-numbered carbon chains were present in these lipids. Only 1 alcohol (C22:0) was detected in the internal lipids of C. vomitoria larvae, while just 4 alcohols from – C18:0 to C24:0 – were identified in the internal lipids of pupae, and males and females. We also identified glycerol and cholesterol in the larvae, pupae, males and females of C. vomitoria. The individual alcohols and FAMEs, as well as their mixtures isolated from the cuticular and internal lipids of larvae, pupae, males and females of C. vomitoria, demonstrated antimicrobial activity against entomopathogenic fungi.

Keywords

antifungal activitycuticular lipidsinternal lipidsfatty acid methyl estersalcoholsCalliphora vomitoria
Type
Research Article
Information
Parasitology , Volume 140 , Issue 8 , July 2013 , pp. 972 - 985
Copyright
Copyright © Cambridge University Press 2013 

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