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Cortical granule exocytosis in Bufo arenarum oocytes matured in vitro

Published online by Cambridge University Press:  06 August 2001

J. Oterino
Affiliation:
Departamento de Biologia del Desarrollo, Chacabuco 461, 4000 San Miguel de Tucumán, Argentina
G. Sánchez Toranzo
Affiliation:
Departamento de Biologia del Desarrollo, Chacabuco 461, 4000 San Miguel de Tucumán, Argentina
L. Zelarayán
Affiliation:
Departamento de Biologia del Desarrollo, Chacabuco 461, 4000 San Miguel de Tucumán, Argentina
J.N. Valz-Gianinet
Affiliation:
Departamento de Biologia del Desarrollo, Chacabuco 461, 4000 San Miguel de Tucumán, Argentina
M.I. Bühler
Affiliation:
Departamento de Biologia del Desarrollo, Chacabuco 461, 4000 San Miguel de Tucumán, Argentina

Abstract

Denuded Bufo arenarum oocytes matured in vitro by progesterone treatment exhibited abnormal segmentation due to the penetration of more than one sperm. These oocytes were able to respond to activation stimuli and exhibited the external signs characteristic of activation. However, the prevention of polyspermy was not effective in these oocytes, which exhibited numerous sperm in their cytoplasm. The aim of this work was to analyse the cortical reaction in polyspermic Bufo arenarum oocytes matured in vitro. The result indicate that the cortical reaction of these oocytes seems to occur with a chronological sequence similar to that described for ovoposited oocytes of this species. In addition, when, 1 min after pricking, cortical granule exocytosis occurred, the oocytes became refractory to sperm entry, suggesting that they are able to establish a slow block to polyspermy.

Type
Research Article
Copyright
© 2001 Cambridge University Press

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