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Effects of repeated gestation and lactation on milk n-6 fatty acid composition in rats fed on a diet rich in 18:2n-6 or 18:3n-6

Published online by Cambridge University Press:  09 March 2007

Yung-Sheng Huang ,
Peter R. Redden ,
David F. Horrobin ,
Sandra Churchill ,
Barbara Parker ,
Ron P. Ward  and
David E. Mills
Yung-Sheng Huang
Affiliation:
Efamol Research Institute, Kentville, Nova Scotia B4N 4H8, Canada
Peter R. Redden
Affiliation:
Efamol Research Institute, Kentville, Nova Scotia B4N 4H8, Canada
David F. Horrobin
Affiliation:
Efamol Research Institute, Kentville, Nova Scotia B4N 4H8, Canada
Sandra Churchill
Affiliation:
Efamol Research Institute, Kentville, Nova Scotia B4N 4H8, Canada
Barbara Parker
Affiliation:
Efamol Research Institute, Kentville, Nova Scotia B4N 4H8, Canada
Ron P. Ward
Affiliation:
Department of Health Studies, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada
David E. Mills
Affiliation:
Department of Health Studies, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada
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Abstract

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The present study examined the effect of repeated gestation and lactation on the levels of long-chain n-6 polyunsaturated fatty acids in rat milk fat, and examined whether such levels might be modulated by supplementing the diet of the lactating dams with either (g/kg) 50 safflower oil (SFO; containing 800 g 18:2n-6/kg), or 50 evening primrose oil (EPO; containing 720 g 18:2n-6 and 90 g 18:3n-6/kg). The milk was collected at three different times (days 1, 8 and 15) in each given lactation period from female Sprague-Dawley rats which were successively bred for four pregnancies and lactations. Results showed that dietary fat and breeding frequency had no significant effects on milk triacylglycerol content, but they modified the pattern of milk fatty acids in both triacylglycerol and phospholipid fractions. After three or four successive breedings rats fed on EPO produced milk containing less saturated but more monounsaturated and polyunsaturated fatty acids compared with those fed on SFO. During the course of lactation the levels of n-6 metabolites, e.g. 18:3n-6, 20:3n-6 and 20:4n-6, in milk fat declined progressively. However, they were consistently higher in the EPO group than in the SFO group. These findings suggest that the levels of long-chain n-6 metabolites in the milk fat may be increased through supplementing the maternal diet with 18:3n-6.

Keywords

Maternal dietMilk triacylglycerolsn-6 Polyunsaturated fatty acids
Type
Effects of Dietary Fat on Milk Lipids
Information
British Journal of Nutrition , Volume 68 , Issue 2 , September 1992 , pp. 337 - 347
Copyright
Copyright © The Nutrition Society 1992

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