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Nanoscale Forces at the Heart of Staphylococcus Infections

Published online by Cambridge University Press:  01 February 2011

Ruchirej Yongsunthon ,
Francis Paul Vellano ,
Brian H. Lower ,
Vance G. Fowler ,
Emily Alexander  and
Steven K. Lower
Ruchirej Yongsunthon
Affiliation:
yongsuntr@Corning.com, Corning Incorporated, Molecular Spectroscopy and Separations, Corning Incorporated, SP-FR-01, R1S12L/23L, Corning, NY 14831, Corning, NY, 14831, United States
Francis Paul Vellano
Affiliation:
fvellano@buffalo.edu, Ohio State University, Columbus, OH, 43210, United States
Brian H. Lower
Affiliation:
brian.lower@pnl.gov, Pacific Northwest National Laboratory, Richland, WA, 99352, United States
Vance G. Fowler
Affiliation:
fowle003@mc.duke.edu, Duke University, Durham, NC, 27710, United States
Emily Alexander
Affiliation:
emily.alexander@duke.edu, Duke University, Durham, NC, 27710, United States
Steven K. Lower
Affiliation:
lower.9@osu.edu, Ohio State University, Columbus, OH, 43210, United States
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Abstract

Staphylococcus aureus is one of the most frequently isolated bacteria from infected medical implants. S. aureus has the capacity to adhere to the surface of an implant where it forms a biofilm. We used atomic force microscopy to probe binding forces between a fibronectin-coated tip and isolates of S. aureus, which were obtained from either patients with infected prostheses or healthy humans. A unique force-signature was observed for binding events between the tip and the cells. There is a strong distinction (p=0.01) in the binding force-signature observed for S. aureus isolated from the infected vs. healthy populations. This observation suggests a fundamental correlation between nanometer scale binding forces and the clinical outcome of patients with implanted medical devices.

Keywords

biomedicalscanning probe microscopy (SPM)biological
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1025: Symposium B – Nanoscale Phenomena in Functional Materials by Scanning Probe Microscopy , 2007 , 1025-B04-10
Copyright
Copyright © Materials Research Society 2008

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References

1. Darouiche, R. O., New Eng. J. Med. 350, 1422–1429 (2004)Google Scholar
2. Chamis, A. L.; Peterson, G. E.; Cabell, C. H.; Corey, G. R.; Sorrentino, R. A.; Greenfield, R. A.; Ryan, T.; Reller, L. B.; Fowler, V. G., Circulation 104, 1029–1033 (2001)Google Scholar
3. Mylonakis, E.; Calderwood, S. B., New Eng. J. Med. 345, 1318–1330 (2001)Google Scholar
4. El-Ahdab, F.; Benjamin, D. K.; Wang, A.; Cabell, C. H.; Chu, V. H.; Stryjewski, M. E.; Corey, G. R.; Sexton, D. J.; Reller, L. B.; Fowler, V. G., Amer. J. Med. 118, 225–229 (2005)Google Scholar
5. Greene, C.; McDevitt, D.; Francois, P.; Vaudaux, P. E.; Lew, D. P.; Foster, T. J., Mol. Microbiol. 17, 1143–1152 (1995)Google Scholar
6. Foster, T., Staphylococcus. In Medical Microbiology, 4th ed.; Baron, S., Ed. The University of Texas Medical Branch at Galveston: Galveston, TX; Chapter 12 (1996)Google Scholar
7. Kuusela, , Nature 276, 718–720 (1978)Google Scholar
8. Proctor, R. A.; Mosher, D. F.; Olbrantz, P. J., J. Biol. Chem. 257, 14788–14794 (1982)Google Scholar
9. Foster, T. J.; Hook, M., Trends Microbiol. 6, 484–488 (1998)Google Scholar
10. Wann, E. R.; Gurusiddappa, S.; Hook, M., J. Biol. Chem., 275, 13863–13871 (2000)Google Scholar
11. Massey, R. C.; Kantzanou, M. N.; Fowler, T.; Day, N. P. J.; Schofield, K.; Wann, E. R.; Berendt, A. R.; Hook, M.; Peacock, S. J., Cell. Microbiol., 3, 839–851 (2001)Google Scholar
12. Schwarz-Linek, U.; Werner, J. M.; Pickford, A. R.; Gurusiddappa, S.; Kim, J. H.; Pilka, E. S.; Briggs, J. A. G.; Gough, T. S.; Hook, M.; Campbell, I. D.; Potts, J. R., Nature 423, 177–181 (2003)Google Scholar
13. Prince, J. L.; Dickinson, R. B., Langmuir 19, 154–159 (2003)Google Scholar
14. Simpson, K. H.; Bowden, G.; Hook, M.; Anvari, B., J. Bacteriol. 185, 2031–2035 (2003)Google Scholar
15. Yongsunthon, R.; Lower, S. K., Force measurements between a bacterium and another surface in situ. Adv. Appl. Microbiol. 58, 97–124 (2006)Google Scholar
16. Yongsunthon, R.; Lower, S. K., J. Elec. Spectro. Rel. Phenom. 150, 228–234 (2006)Google Scholar
17. Yongsunthon, R.; Vance, G. Fowler, J.; Lower, B. H.; Vellano, F. P.; Alexander, E.; Reller, L. B.; Corey, G. R.; Lower, S. K., Langmuir 23(5): 2289–2292 (2007)Google Scholar
18. Gross, M.; Cramton, S. E.; Gotz, F.; Peschel, A., Inf. Imm. 69, 3423–3426 (2001)Google Scholar
19. Oberdorfer, Y.; Fuchs, H.; Janshoff, A., Langmuir 16, 9955–9958 (2000)Google Scholar
20. Lo, Y. S.; Huefner, N. D.; Chan, W. S.; Dryden, P.; Hagenhoff, B.; Beebe, T. P., Langmuir 15, 6522–6526 (1999)Google Scholar
21. Craig, V. S. J.; Neto, C., Langmuir 17, 6018–6022 (2001)Google Scholar
22. Lower, B. H.; Yongsunthon, R.; Vellano, F. P.; Lower, S. K., J. Bacteriol. 187, 2127–2137 (2005)Google Scholar