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Isolation and characterization of microsatellite markers for the red alga Porphyra umbilicalis

Published online by Cambridge University Press:  14 December 2017

Elena Varela-Álvarez Open the ORCID record for Elena Varela-Álvarez [Opens in a new window] ,
Ana C. Balau ,
Cristina Paulino ,
Estibaliz Berecibar ,
Gareth A. Pearson  and
Ester A. Serrão
Elena Varela-Álvarez*
Affiliation:
CCMAR-CIMAR, Centro de Ciências do Mar, Laboratório Associado, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
Ana C. Balau
Affiliation:
CCMAR-CIMAR, Centro de Ciências do Mar, Laboratório Associado, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
Cristina Paulino
Affiliation:
CCMAR-CIMAR, Centro de Ciências do Mar, Laboratório Associado, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
Estibaliz Berecibar
Affiliation:
Task Group for the Extension of the Continental Shelf (EMEPC). Rua Costa Pinto 165, Paço de Arcos 2770-047, Apartado 1139, Portugal
Gareth A. Pearson
Affiliation:
CCMAR-CIMAR, Centro de Ciências do Mar, Laboratório Associado, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
Ester A. Serrão
Affiliation:
CCMAR-CIMAR, Centro de Ciências do Mar, Laboratório Associado, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
*
*Corresponding author. E-mail: evarela@ualg.pt
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Abstract

The red algal genus Porphyra is sister to the genus Pyropia, the single most valuable marine crop in the Orient. We developed microsatellite loci for the red alga Porphyra umbilicalis, a widespread species in the Northern Atlantic. Enriched DNA libraries were constructed and 68 loci were screened for amplification and polymorphism. Seven polymorphic microsatellite markers were isolated using 44 individuals collected from four natural populations. The number of alleles per locus ranged from two to 12. Null alleles were detected in three loci. Among the markers reported, we tested also cross-amplification with two other Porphyra spp. These polymorphic microsatellite markers should be useful for investigating population genetic structure of P. umbilicalis in the North East Atlantic.

Keywords

Bangialesgenetic diversityNoriNorth Atlanticpopulation structurered algae
Type
Short Communication
Information
Plant Genetic Resources , Volume 16 , Issue 4 , August 2018 , pp. 390 - 393
Copyright
Copyright © NIAB 2017 

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