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Direct Observation of Calcium Oxalate Monohydrate Precipitation at Phospholipid Monolayers with Brewster Angle Microscopy

Published online by Cambridge University Press:  15 February 2011

Isa O. Benítez ,
Rénal Backov ,
Saeed R. Khan  and
Daniel R. Talham
Isa O. Benítez
Affiliation:
Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200 and
Rénal Backov
Affiliation:
Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200 and
Saeed R. Khan
Affiliation:
Department of Pathology, University of Florida, Gainesville, Florida 32610.
Daniel R. Talham
Affiliation:
Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200 and
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Abstract

The precipitation of calcium oxalate monohydrate (COM) at phospholipid monolayers has been observed in-situ by Brewster angle microscopy (BAM). A monolayer of 1,2-dipalmitoylsn-glycero-3-phosphocholine (DPPC) compressed to a LC state over a calcium oxalate subphase shows the growth of COM as very bright spots. The identity of COM was confirmed in a transferred film by scanning electron microscopy. BAM can also be used to determine where COM precipitates when the monolayer has two phases at equilibrium. Monolayers of DPPC and 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (DPPG) crystallize COM at phase boundaries. In addition, phase separated binary phospholipid mixtures of DPPC and 1,2-dimiristoyl-sn-glycero-3-phosphocholine (DMPC) have been prepared and monitored by BAM. The crystal growth in this case is confined to domains of DPPC due to its ability to form a liquid condensed phase.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 774: Symposium O – Materials Inspired by Biology , 2003 , O5.9
Copyright
Copyright © Materials Research Society 2003

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