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Cloning, molecular characterization, and spatial and developmental expression analysis of GPR41 and GPR43 genes in New Zealand rabbits

Published online by Cambridge University Press:  28 February 2017

C. Y. Fu ,
L. Liu ,
Q. Gao ,
X. Y. Sui  and
F. C. Li
C. Y. Fu
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong 271018, China
L. Liu
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong 271018, China
Q. Gao
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong 271018, China
X. Y. Sui
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong 271018, China
F. C. Li*
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong 271018, China
*
†E-mail: chlf@sdau.edu.cn
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Abstract

Short-chain fatty acids (SCFAs) play a regulatory role in various physiological processes in mammals and act as endogenous ligands for the G protein-coupled receptors (GPR) 41 and 43. The role of GPR41 and GPR43 in mediating SCFA signaling in the rabbit remains unclear. The present study was to investigate the sequence of the GPR41 and GPR43 messenger RNA (mRNA) and their expression pattern in different tissues and developmental stages in New Zealand rabbit. Comparison of genomic sequences in GenBank using the Basic Local Alignment Search Tool program suggested that the New Zealand rabbit GPR41 mRNA has high similarities with the human (84%), bovine (84%) and Capra hircus (84%) genes. Similarly, GPR43 mRNA has high similarity with the rat (84%) and mouse (84%) genes. Real-time PCR results indicated that GPR41 and GPR43 mRNA were expressed throughout rabbit’s whole development and were expressed in several tissues. G protein-coupled receptor 41 and GPR43 mRNA were most highly expressed in pancreas (P<0.05) and s.c. adipose tissue (P<0.05), respectively. The expression levels of GPR41 mRNA was down-regulated in duodenum, cecum (P<0.05) and pancreas and up-regulated in jejunum, ileum, adipose tissue and spleen during growth. G protein-coupled receptor 43GPR43 mRNA was highly expressed in the duodenum, jejunum, ileum, colon, cecum and lung at 15th day (P<0.05), whereas the expression levels in the pancreas and spleen increased later after birth, with the highest expression at 60th day (P<0.05).

Keywords

GPR41GPR43gene sequencesNew Zealand rabbitshort-chain fatty acids
Type
Research Article
Information
animal , Volume 11 , Issue 10 , October 2017 , pp. 1798 - 1806
Copyright
© The Animal Consortium 2017 

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Footnotes

a

Co-first authors.

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