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The Expression of FOXJ1 in Neurogenesis after Transient Focal Cerebral Ischemia

Published online by Cambridge University Press:  23 September 2014

Yabo Huang ,
Zheng Xu ,
Jie Cao ,
Haibo Cao  and
Shiming Zhang
Yabo Huang
Affiliation:
Department of Neurosurgery, First Affiliated Hospital of Soochow University, Suzhou, China
Zheng Xu
Affiliation:
Department of Neurosurgery, First Affiliated Hospital of Soochow University, Suzhou, China
Jie Cao
Affiliation:
Department of Neurosurgery, First Affiliated Hospital of Soochow University, Suzhou, China
Haibo Cao
Affiliation:
Department of Neurosurgery, First Affiliated Hospital of Soochow University, Suzhou, China
Shiming Zhang*
Affiliation:
Department of Neurosurgery, First Affiliated Hospital of Soochow University, Suzhou, China
*
Department of Neurosurgery, the First Affiliated Hospital of Soochow University, Suzhou, 215006, China. Email: zhang.shiming@yahoo.cn.
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Abstract

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Objective and Background:

FOXJ1 is a member of the Forkhead/winged-helix (Fox) family of transcription factors, which is required for the differentiation of the cells acting as adult neural stem cells which participate in neurogenesis and give rise to neurons, astrocytes, oligodendrocytes. The expression pattern of FOXJ1 in the brain after cerebral ischemia has so far not been described. In the current study, we investigated the expression pattern of FOXJ1 in the rat brain after cerebral ischemia by animal model.

Methods:

We performed a middle cerebral artery occlusion (MCAO) model in adult rats and investigated the expression of FOXJ1 in the brain by Western blotting and immunochemistry; double immunofluorescence staining was used to analyze FOXJ1's co-expression with Ki67.

Results:

Western blot analysis showed that the expression of FOXJ1 was lower than normal and sham-operated brain after cerebral ischemia, but the level of FOXJ1 gradually increased from Day 1 to Day 14. Immuohistochemical staining suggested that the immunostaining of FOXJ1 deposited strongly in the ipsilateral and contralateral hemisphere in the cortical penumbra (CP). There was no FOXJ1 expression in the ischemic core (IC). The positive cells in the cortical penumbra might migrat to the ischemic core. In addition, double immunofluorescence staining revealed that FOXJ1 was co-expressed with mAP-2 and gFAP, and Ki67 had the colocalization with NeuN, GFAP, and FOXJ1.

Conclusions:

All our findings suggest that FOXJ1 plays an important role on neuronal production and neurogenesis in the adult brain after cerebral ischemia.

Résumé:

Résumé:Objectif et contexte:

FOXJ1 est un membre de la famille de facteurs de transcription Forkhead/winged-helix (Fox). Il est requis pour la différenciation des cellules agissant comme cellules souches nerveuses adultes qui participent à la neurogenèse et donnent naissance à des neurones, des astrocytes et des oligodendrocytes. l'expression de FOXJ1 dans le cerveau suite à un accès ischémique focal transitoire n'a pas été décrite à ce jour. Dans cette étude, nous avons exploré l'expression de FOXJ1 dans un modèle animal, le cerveau de rat, suite à une ischémie cérébrale.

Méthode:

Nous avons effectué une occlusion de l'artère cérébrale moyenne chez des rats adultes et nous avons examiné l'expression de FOXJ1 dans le cerveau par buvardage Western et immunohistochimie. Nous avons utilisé le double marquage par immunofluorescence pour analyser la coexpression de FOXJ1 et de Ki67.

Résultats:

l'analyse par buvardage Western a montré que l'expression de FOXJ1 était plus faible dans le cerveau de rat qui avait subi une ischémie cérébrale que dans le cerveau normal ou le cerveau qui avait subi une intervention factice. Cependant, le niveau de FOXJ1 augmentait graduellement du jour l au jour 14 après l'ischémie. La coloration immunohistochimique de FOXJ1 était intense dans la zone de pénombre corticale de l'hémisphère ipsi et contralatéral. il n'y avait pas d'expression de FOXJ1 dans le centre de la zone ischémique. Il est possible que les cellules positives dans la zone de pénombre corticale puissent migrer vers le centre de la zone ischémique. De plus, une double coloration par immunofluorescence a montré une coexpression de FOXJ1 et mAP-2 et gFAP, et une colocalisation de Ki67 et de NeuN, gFAP et FOXJ1.

Conclusions:

Ces observations sont en faveur d'un rôle important de FOXJ1 dans la production neuronale et la neurogenèse dans le cerveau adulte à la suite d'une ischémie cérébrale.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 40 , Issue 3 , May 2013 , pp. 403 - 409
Copyright
Copyright © The Canadian Journal of Neurological 2013

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