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The bioactivities of the central segment of Zp2 polypeptide

Published online by Cambridge University Press:  19 May 2016

Zuowu Cao ,
Chuncheng Nie ,
Yan Xie  and
Dongqin Cai
Zuowu Cao*
Affiliation:
Department of Developmental and Regenerative Biology, Jinan University, Guangzhou 510632, China. Department of Developmental and Regenerative Biology, Jinan University, Guangzhou 510632, China.
Chuncheng Nie
Affiliation:
Department of Developmental and Regenerative Biology, Jinan University, Guangzhou 510632, China.
Yan Xie
Affiliation:
Department of Developmental and Regenerative Biology, Jinan University, Guangzhou 510632, China.
Dongqin Cai
Affiliation:
Department of Developmental and Regenerative Biology, Jinan University, Guangzhou 510632, China. Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, China.
*
All correspondence to: Zuowu Cao. Department of Developmental and Regenerative Biology, Jinan University, Guangzhou 510632, China. E-mail: caozuowu@ymail.com
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Summary

In order to understand the role of the protein zona pellucida 2 in fertilization, an antibody against a central segment of the zona pellucida 2 peptide, segment 190–505 (Z2eH), was prepared. The influence of the antibody on sperm–zona interaction was tested using the sperm–egg binding assay. The effect of the antibody on fertility was evaluated by passive immunization with anti-Z2eH antibody. Immunohistochemical assay showed that an antibody from rabbit reacted specifically with the natural zona pellucida on mouse ovarian sections. Immunofluorescence assay showed that the antibody bound specifically to the zonae pellucidae of the ovulated oocytes and 2-cell embryos after passive immunization. The antibody-treated oocytes bound capacitated sperm as control oocytes, passive immunization did not impede the action of sperm to fertilize the oocyte in vivo. These findings suggest that the central peptide of ZP2 (190–505) is immunogenic and contains zona pellucida-specific epitopes, however the central polypeptide might not be the crucial part from which to construct a functional domain to bind sperm.

Keywords

AntibodyImmunofluorescenceMouse zona pellucida 2 (ZP2)Passive immunizationSperm–egg binding
Type
Research Article
Information
Zygote , Volume 24 , Issue 5 , October 2016 , pp. 768 - 774
Copyright
Copyright © Cambridge University Press 2016 

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