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Anodic Aluminum Oxide (AAO) Membranes for Neurite Outgrowth

Published online by Cambridge University Press:  04 February 2013

Meghan E. Casey ,
Anthony P. Ventura ,
Wojciech Z. Misiolek  and
Sabrina Jedlicka
Meghan E. Casey
Affiliation:
Bioengineering
Anthony P. Ventura
Affiliation:
Materials Science and Engineering Institute for Metal Forming
Wojciech Z. Misiolek
Affiliation:
Materials Science and Engineering Institute for Metal Forming
Sabrina Jedlicka
Affiliation:
Bioengineering Materials Science and Engineering Center for Advanced Materials and Nanotechnology Lehigh University, Bethlehem, PA 18015 USA
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Abstract

Anodic aluminum oxide (AAO) membranes were fabricated in a mild two-step anodization procedure. The voltage was varied during both anodization steps to control the pore size and morphology of the AAO membranes. Pore sizes ranged from 34 nm to 117 nm. Characterization of the pore structure was performed by scanning electron microscopy (SEM). To assess the potential of the AAO membranes as a neuronal differentiation platform, C17.2 neural stem cells (NSCs), an immortalized and multipotent cell line, were used. The NSCs were forced to differentiate via serum-withdrawal. Cellular growth was characterized by immunocytochemistry (ICC) and SEM. ImageJ software was used to obtain phenotypic cell counts and neurite outgrowth lengths. Results indicate a highly tunable correlation between AAO nanopore sizes and differentiated cell populations. By selecting AAO membranes with specific pore size ranges, control of neuronal network density and neurite outgrowth length was achieved.

Keywords

biomaterialceramicbiological
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium L – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 97 - 102
Copyright
Copyright © Materials Research Society 2012 

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