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Maternal group B Streptococcus and the infant gut microbiota

Part of: The Gut Microbiome: How it is Shaped by Early Life

Published online by Cambridge University Press:  12 August 2015

A. E. Cassidy-Bushrow Open the ORCID record for A. E. Cassidy-Bushrow [Opens in a new window] ,
A. Sitarik ,
A. M. Levin ,
S. V. Lynch ,
S. Havstad ,
D. R. Ownby ,
C. C. Johnson  and
G. Wegienka
A. E. Cassidy-Bushrow*
Affiliation:
Department of Public Health Sciences, Henry Ford Hospital, One Ford Place, Detroit, MI, USA Center for Allergy, Asthma and Immunology Research, Henry Ford Hospital, Detroit, MI, USA
A. Sitarik
Affiliation:
Department of Public Health Sciences, Henry Ford Hospital, One Ford Place, Detroit, MI, USA Center for Allergy, Asthma and Immunology Research, Henry Ford Hospital, Detroit, MI, USA
A. M. Levin
Affiliation:
Department of Public Health Sciences, Henry Ford Hospital, One Ford Place, Detroit, MI, USA Center for Allergy, Asthma and Immunology Research, Henry Ford Hospital, Detroit, MI, USA
S. V. Lynch
Affiliation:
Department of Medicine, University of California, San Francisco, CA, USA
S. Havstad
Affiliation:
Department of Public Health Sciences, Henry Ford Hospital, One Ford Place, Detroit, MI, USA Center for Allergy, Asthma and Immunology Research, Henry Ford Hospital, Detroit, MI, USA
D. R. Ownby
Affiliation:
Center for Allergy, Asthma and Immunology Research, Henry Ford Hospital, Detroit, MI, USA Department of Pediatrics, Division of Allergy and Clinical Immunology, Georgia Regents University, Augusta, GA, USA
C. C. Johnson
Affiliation:
Department of Public Health Sciences, Henry Ford Hospital, One Ford Place, Detroit, MI, USA Center for Allergy, Asthma and Immunology Research, Henry Ford Hospital, Detroit, MI, USA
G. Wegienka
Affiliation:
Department of Public Health Sciences, Henry Ford Hospital, One Ford Place, Detroit, MI, USA Center for Allergy, Asthma and Immunology Research, Henry Ford Hospital, Detroit, MI, USA
*
*Address for Correspondence: A. E. Cassidy-Bushrow, PhD, MPH, Department of Public Health Sciences, Henry Ford Hospital, 1 Ford Place, 5C, Detroit, MI 48202, USA. (Email acassid1@hfhs.org)
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Abstract

Early patterns of gut colonization may predispose children to adult disease. Exposures in utero and during delivery are associated with the infant gut microbiome. Although ~35% of women carry group B strep (GBS; Streptococcus agalactiae) during pregnancy, it is unknown if GBS presence influences the infant gut microbiome. As part of a population-based, general risk birth cohort, stool specimens were collected from infant’s diapers at research visits conducted at ~1 and 6 months of age. Using the Illumina MiSeq (San Diego, CA) platform, the V4 region of the bacterial 16S rRNA gene was sequenced. Infant gut bacterial community compositional differences by maternal GBS status were evaluated using permutational multivariate analysis of variance. Individual operational taxonomic units (OTUs) were tested using a zero-inflated negative binomial model. Data on maternal GBS and infant gut microbiota from either 1 (n=112) or 6-month-old stool (n=150) specimens was available on 262 maternal-child pairs. Eighty women (30.5%) were GBS+, of who 58 (72.5%) were given intrapartum antibiotics. After adjusting for maternal race, prenatal antifungal use and intrapartum antibiotics, maternal GBS status was statistically significantly associated with gut bacterial composition in the 6 month visit specimen (Canberra R2=0.008, P=0.008; Unweighted UniFrac R2=0.010, P=0.011). Individual OTU tests revealed that infants of GBS+ mothers were significantly enriched for specific members of the Clostridiaceae, Ruminococcoceae, and Enterococcaceae in the 6 month specimens compared with infants of GBS- mothers. Whether these taxonomic differences in infant gut microbiota at 6 months lead to differential predisposition for adult disease requires additional study.

Keywords

antibioticsdevelopmental originsgroup B strepgut microbiota
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 7 , Supplement 1: Themed Issue: The Gut Microbiome and Immunity: How it is Shaped by Early Life , February 2016 , pp. 45 - 53
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Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2015 

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