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Cell Specific Targeting of Multifunctional γ-Fe2O3 Nanoparticles Through Surface Binding of dsDNA

Published online by Cambridge University Press:  26 February 2011

Wolfgang Tremel ,
Mohammed Ibrahim Shukoor ,
Filipe Natalio ,
Muhammad Nawaz Tahir ,
Werner E. G. Müller  and
Heinz Christoph Schröder
Wolfgang Tremel
Affiliation:
tremel@uni-mainz.de, Johannes Gutenberg-Universität Mainz, Institut für Anorganische Chemie und Analytische Chemie, Duesbergweg 10-14, Mainz, 55099, Germany, +49 6131 39-25135, +49 6131 39-25605
Mohammed Ibrahim Shukoor
Affiliation:
ibrahim_shukoor@yahoo.com, Johannes Gutenberg-Universität, Institut für Anorganische Chemie und Analytische Chemie, Duesbergweg 10-14, Mainz, 55099, Germany
Filipe Natalio
Affiliation:
natalio@uni-mainz.de, Johannes Gutenberg-Universität, Institut für Biochemie und Pathobiochemie, Duesbergweg 6, Mainz, 55099, Germany
Muhammad Nawaz Tahir
Affiliation:
tahir@uni-mainz.de, Johannes Gutenberg-Universität, Institut für Anorganische Chemie und Analytische Chemie, Duesbergweg 10-14, Mainz, 55099, Germany
Werner E. G. Müller
Affiliation:
wmueller@uni-mainz.de, Johannes Gutenberg-Universität, Institut für Biochemie und Pathobiochemie, Duesbergweg 6, Mainz, 55099, Germany
Heinz Christoph Schröder
Affiliation:
hschroed@uni-mainz.de, Johannes Gutenberg-Universität, Institut für Biochemie und Pathobiochemie, Duesbergweg 6, Mainz, 55099, Germany
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Abstract

The immobilization of polyinosinic-polycytidylic acid [poly(IC)] on ã-Fe2O3 maghemite nanoparticles via the phosphor-amidate route using a multifunctional polymer is reported. The dsRNA coupled nanoparticles were used to visualize the Toll-like (TLR3) receptors at the cell surface. The presence of TLR3 was demonstrated independently in the Caki-1 cell line by RT-PCR and immunostaining techniques

Keywords

biomedicalnanoscalesurface chemistry
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1032: Symposium I – Nanoscale Magnetic Materials and Applications , 2007 , 1032-I15-05
Copyright
Copyright © Materials Research Society 2008

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