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Design and Synthesis of a Multifunctional Probe for Bio-Imaging and Therapeutics

Published online by Cambridge University Press:  01 February 2011

Yu-San Liu ,
Chi Hui Liang ,
P. Thomas Vernier ,
Yinghua Sun  and
Martin A. Gundersen
Yu-San Liu
Affiliation:
yusanliu@usc.edu, University of Southern California, Mork Family Department of Chemical Engineering and Materials Science, 3737 Watt Way, PHE512, Los Angeles, California, 90089, United States
Chi Hui Liang
Affiliation:
chiliang@usc.edu, University of Southern California, Mork Family Department of Chemical Engineering and Materials Science, Los Angeles, California, 90089, United States
P. Thomas Vernier
Affiliation:
vernier@mosis.org, University of Southern California, Department of Electrical Engineering-Electrophysics, Los Angeles, California, 90089, United States
Yinghua Sun
Affiliation:
yinghuas@usc.edu, University of Southern California, Mork Family Department of Chemical Engineering and Materials Science, Los Angeles, California, 90089, United States
Martin A. Gundersen
Affiliation:
mag@usc.edu, University of Southern California, Mork Family Department of Chemical Engineering and Materials Science, Los Angeles, California, 90089, United States
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Abstract

We report the synthesis of CdSe/ZnSe/ZnS (core/shell/shell) quantum dots (QDs) as a fluorescence probe for biological cells, and their functionalization with carbon nanotube (CNT)-retrovirus complexes (labeled with the QDs) to form multi-functional tool with potential applications for cancer therapeutics. A mild method for preparing this QD-CNT-retrovirus complex, which preserves viral function, is reported. Observations and imaging under fluorescence confocal microscope show the expression of enhanced green fluorescent protein (EGFP) gene and QD uptake in viable cells, indicating that this novel probe is able to enter cells, crossing the natural barrier of the cell membranes. Such multi-functional probes have potential as medical diagnostics and for therapeutics for diseases including cancer. The multifunctional probe is of interest for potentially broad applications – with the quantum dots as the visualization aid, carbon nanotubes as an efficient opto-thermo converter for selective extermination of cells with the help of light irradiation at near-IR range, and the retrovirus as a vehicle for genetic therapy.

Keywords

core/shellbiologicalnanostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 943: Symposium Y – Nanostructured Probes for Molecular Bio-Imaging , 2006 , 0943-Y03-03
Copyright
Copyright © Materials Research Society 2006

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