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The Synthesis and Characterization of Nano-hydroxyapatite (nHAP)-g-poly(lactide-co-glycolide)-g-collagen Polymer for Tissue Engineering Scaffolds

Published online by Cambridge University Press:  25 June 2013

Didarul Bhuiyan
Affiliation:
Department of Biomedical Engineering and University of Alabama at Birmingham, Birmingham, AL 35924
Michael J. Jablonsky
Affiliation:
Department of Chemistry, University of Alabama at Birmingham, Birmingham, AL 35924
John Middleton
Affiliation:
Department of Biomedical Engineering and University of Alabama at Birmingham, Birmingham, AL 35924
Rina Tannenbaum
Affiliation:
Department of Biomedical Engineering and University of Alabama at Birmingham, Birmingham, AL 35924 UAB Comprehensive Cancer Center and the School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35924
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Abstract

Bone grafts, commonly performed to augment bone regeneration from autologous or alleogeneic sources, carry an enormous cost, estimated at upwards of 21 billion dollars per year. Hydroxyapatite (HAP) bio-ceramic has been widely used in clinic as a bone graft substitute material due to its biocompatibility and the similarity of its structure and composition to bone mineral. However, its applications are limited due to its lack of strength and toughness. Researchers have attempted to overcome these issues by combining HAP bio-ceramics into resorbable polymers to improve their mechanical properties. However, poor bonding between the HAP and the polymer caused separation at the polymer-filler interface. To overcome this, short chains of polymers were grafted directly from the hydroxyl groups on the surface of nanocrystalline HAP. Collagens, being the most abundant proteins in the body, and having suitable properties such as biodegradability, bioabsorbability with low antigenicity, high affinity to water, and the ability to interact with cells through integrin recognition, makes them a very promising candidate for the modification of the polymer surface. In this study, a novel method of synthesizing nano-hydroxyapatite (nHAP)-g-poly(lactide-co-glycolide)-g-collagen polymer was introduced. The synthesis process was carried out in several steps. First, poly (lactide-co-glycolide) (PLGA) polymer was directly grafted onto the hydroxyl group of the surface of n-HAP particles by ring-opening polymerization, and subsequently coupled with succinic anhydride. In order to activate the co-polymer for collagen attachment, the carboxyl end group obtained from succinic anhydride was reacted with N-hydroxysuccinimide (NHS) in the presence of dicyclohexylcarbodiimide (DCC) as the cross-linking agent. Finally, the activated co-polymer was attached to calf skin collagen type I, in hydrochloric acid/phosphate buffer solution and the precipitated co-polymer with attached collagen was isolated. The synthesis was monitored by 1H NMR and FTIR spectroscopies and the products after each step were characterized by thermal analysis (TGA and DSC). These composite materials will be tested as potential scaffolds for tissue engineering applications.

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Articles
Copyright
Copyright © Materials Research Society 2013 

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References

REFERENCES

Branemark, P. I.; Worthington, P.; Grondahl, K. Osseointegration and Autogenous Onlay Bone Grafts: Reconstruction of the Edentulous Atrophic Maxilla; Quintessence Pub Co.: Chicago, IL, 2001, pp 48.Google Scholar
Beauchamp, D. R.; Evers, M.B.; Mattox, K. L.; Townsend, C. M.; Sabiston, D. C. Sabiston Textbook of Surgery: The Biological Basis of Modern Surgical Practice, 19th ed. W B Saunders Co.: London, 2012, pp 783791 Google Scholar
Melton, L. J. Bone 1993, 14(1), 18 CrossRefGoogle Scholar
Flierl, M. A.; Gerhardt, D.C.; Hak, D.J.; Morgan, S. J.; Stahel, P. F. Orthopedics 2010, 33(2)CrossRefGoogle Scholar
Giannoudis, P.V.; Dinopoulos, H., Tsiridis, E. Injury 2005. 36, 2027.CrossRefGoogle Scholar
Porjazoska, A.; Yilmaz, O.K.; Baysal, K.; Cvetkovska, M.; Sirvanci, S.; Ercan, F.; Baysal, B. M. J. Biomater. Sci. Polymer Edn. 2006, 17(3), 323340 CrossRefGoogle Scholar
Liao, S.; Wang, W.; Uo, M.; Ohkawa, S.; Akasaka, T.; Tamura, K.; Cui, F.; Watari, F. Biomaterials 2005, 26, 75647571 CrossRefGoogle Scholar
Zhang, P.; Hong, Z.; Yu, T.; Chen, X.; Jing, X. Biomaterials 2009, 30, 5870 CrossRefGoogle Scholar
Furukawa, T.; Matsusue, Y.; Yasunaga, T.; Shikinami, Y.; Okuno, M.; Nakamura, T. Biomaterials 2000, 21, 889898 CrossRefGoogle Scholar
Hasegawa, S.; Ishii, S.; Tamura, J.; Furukawa, T.; Neo, M.; Matsusue, Y.; Shikinami, Y.; Okuno, M.; Nakamura, T. Biomaterials 2006, 27, 13271332.CrossRefGoogle Scholar
Shikinami, Y.; Okuno, M. Biomaterials 1999, 20, 859877 CrossRefGoogle Scholar
Shikinami, Y.; Okuno, M. Biomaterials 2001, 22, 31973211 CrossRefGoogle Scholar
Verheyen, C. C.; Wijn, J. R.; Blitterswijk, C. A.; Groot, K. J Biomed Mater Res 1992, 26, 12771296 CrossRefGoogle Scholar
Verheyen, C. C.; Klein, C. P.; Bleick-Hogerovorst, J.M.; Wolke, J.G.; Blitterswijk, C. A.; Groot, K. J Mater Sci: Mat in Med. 1993, 4(1), 5865 Google Scholar
Neffe, A.T.; Loebus, A.; Zaupa, A.; Stoetzel, C.; Mueller, F.A.; Lendlein, A. Acta. Biomater. 2011, 7, 16931701 CrossRefGoogle Scholar
Ignjatovic, N.; Tomic, S.; Dakic, M.; Miljkovic, M.; Plavsic, M.; Uskokovic, D. Biomaterials 1999, 20, 809816 CrossRefGoogle ScholarPubMed
Deng, X.; Hao, J.; Wang, C. Biomaterials 2001, 22, 28672873 CrossRefGoogle Scholar
Edgecombe, B.D.; Stein, J. A.; Frechet, J. M. Macromolecules, 1998, 31, 12921304 CrossRefGoogle Scholar