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Baseline determination and detection of resistance to imidacloprid in Bemisia tabaci (Homoptera: Aleyrodidae)

Published online by Cambridge University Press:  10 July 2009

Matthew Cahill*
Affiliation:
Department of Biological and Ecological Chemistry, IACR – Rothamsted, Harpenden, UK
Kevin Gorman
Affiliation:
Department of Biological and Ecological Chemistry, IACR – Rothamsted, Harpenden, UK
Sara Day
Affiliation:
Department of Biological and Ecological Chemistry, IACR – Rothamsted, Harpenden, UK
Ian Denholm
Affiliation:
Department of Biological and Ecological Chemistry, IACR – Rothamsted, Harpenden, UK
Alfred Elbert
Affiliation:
Bayer AG, Agrochemicals Division, Research Insecticides, Institut für Tiersche Schädlinge, Leverkusen, Germany
Ralf Nauen
Affiliation:
Bayer AG, Agrochemicals Division, Research Insecticides, Institut für Tiersche Schädlinge, Leverkusen, Germany
*
Correspondence: Matthew Cahill, Department of Biological and Ecological Chemistry, IACR – Rothamsted, Harpenden, Herts, AL5 2JQ, UK.

Abstract

Adult Bemisia tabaci (Gennadius) were tested with imidacloprid in a bioassay method using systemically treated cotton leaves. The method was simple, robust and repeatable and provided baseline data for a laboratory and a number of recently collected field strains. The LC50 for imidacloprid susceptible strains was calculated to be 1.7 ppm and a concentration of 16 ppm determined as diagnostic for imidacloprid resistance. Ten strains of B. tabaci collected from the Almeria region of Spain showed significantly less mortality at the diagnostic dose than the susceptible strains. The intensive use of imidacloprid in the Almeria region is considered the reason for the occurrence of resistance in this locality. Resistance to organophosphates, pyrethroids and endosulfan in B-type and non-B type B. tabaci did not confer resistance to imidacloprid.

Type
Original Articles
Copyright
Copyright © Cambridge University Press 1996

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