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Berberine inhibits lipopolysaccharide-induced expression of inflammatory cytokines by suppressing TLR4-mediated NF-ĸB and MAPK signaling pathways in rumen epithelial cells of Holstein calves

Published online by Cambridge University Press:  30 May 2019

Chenxu Zhao ,
Yazhou Wang ,
Xue Yuan ,
Guoquan Sun ,
Bingyu Shen ,
Feng Xu ,
Guyue Fan ,
Meiyu Jin ,
Xinwei Li  and
Guowen Liu
Chenxu Zhao
Affiliation:
Key Laboratory of Zoonoses Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China
Yazhou Wang
Affiliation:
Key Laboratory of Zoonoses Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China
Xue Yuan
Affiliation:
College of Animal Science and Technology, Inner Mongolia National University, Tongliao, Inner Mongolia, 028000, China
Guoquan Sun
Affiliation:
College of Animal Science and Technology, Inner Mongolia National University, Tongliao, Inner Mongolia, 028000, China
Bingyu Shen
Affiliation:
Key Laboratory of Zoonoses Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China
Feng Xu
Affiliation:
Department of Renal Medicine, Second Affiliated Hospital of Jilin University, Changchun, 130041, Jilin, China
Guyue Fan
Affiliation:
Key Laboratory of Zoonoses Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China
Meiyu Jin
Affiliation:
Key Laboratory of Zoonoses Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China
Xinwei Li
Affiliation:
Key Laboratory of Zoonoses Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China
Guowen Liu*
Affiliation:
Key Laboratory of Zoonoses Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China
*
Author for correspondence: Guowen Liu, Email: liuguowen2008@163.com
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Abstract

Subacute ruminal acidosis (SARA) can increase the level of inflammation and induce rumenitis in dairy cows. Berberine (BBR) is the major active component of Rhizoma Coptidis, which is a type of Chinese anti-inflammatory drug for gastrointestinal diseases. The purpose of this study was to investigate the anti-inflammatory effects of BBR on lipopolysaccharide (LPS)-stimulated rumen epithelial cells (REC) and the underlying molecular mechanisms. REC were cultured and stimulated with LPS in the presence or absence of different concentrations of BBR. The results showed that cell viability was not affected by BBR. Moreover, BBR markedly decreased the concentrations and mRNA expression of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin-1β, and interleukin-6 in the LPS-treated REC in a dose-dependent manner. Importantly, Western blotting analysis showed that BBR significantly suppressed the protein expression of toll-like receptor 4 (TLR4) and myeloid differentiation primary response protein (MyD88) and the phosphorylation of nuclear factor-κB (NF-κB), inhibitory kappa B (IκBα), p38 mitogen-activated protein kinase (MAPK), and c-Jun N-terminal kinase (JNK) in LPS-treated REC. Furthermore, the results of immunocytofluorescence showed that BBR significantly inhibited the nuclear translocation of NF-κB p65 induced by LPS treatment. In conclusion, the protective effects of BBR on LPS-induced inflammatory responses in REC may be due to its ability to suppress the TLR4-mediated NF-κB and MAPK signaling pathways. These findings suggest that BBR can be used as an anti-inflammatory drug to treat inflammation induced by SARA.

Keywords

BerberineMAPKNF-κBrumen epithelial cell
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 2 , May 2019 , pp. 171 - 176
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

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Footnotes

*

Chenxu Zhao, and Yazhou Wang contributed equally to this work.

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